General Tissue Culture Supplies

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

NEST BioFactory™

• Temperature Range: Approx. -86° to +64°C
• Sterilized by E-Beam SAL=10-6
• Nonpyrogenic
• Double-layer sterile bag
• TC treated, good for cell attachment and growth
• Clear lot number for batch traceability
• Ultrasonic welding without extrinsic ingredients. Excellent welding line design can avoid producing particles during welding. And make the BioFactory™ stronger.
• Vent caps with 0.22um hydrophobic filters for gas exchange without contamination.
• Wide mouth is applicable to pour culture medium directly. Narrow mouth is applicable to operate with the aseptic pipeline.
• Growth kinetics are the same as cells grow in the cell culture flasks. Applicable to large-scale culture adherent cells.
• Large growing area. Just a single operate, you can cultivate a large number of cells and reduce contamination risk.
• Applicable to automatic machine. Standardize operations to reduce batch differences.

Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies, or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions.

BioFactory™ Layer choices

• 1 Layer
• 2 Layers
• 5 Layers
• 10 Layers
• 40 Layers

Material

USP Class VI grade polystyrene material

• New! Compatible with 6 well plates
• Superior ventilation - prevents clogging/spills
• Advanced design - faster flow through
• Interlocking and stackable
• RNase, DNase, pyrogen and cytotoxin free
• Sterile (gamma radiation) and individually packed
• Designed for use with 50 ml tubes (adaptor for 15 ml tubes included)

The Harvest Premium Cell Strainer system is a fast easy-to-use solution for detaching, isolating, and straining cells from clusters and tissues. A polypropylene frame supports a nylon mesh that comes in three sizes: 40 µm (blue), 70 µm (white), and 100 µm (yellow). A tiered filtration process is made possible by the unique interlocking stackable design.
 
Redesigned with  support tabs (for multi-well plates) and 1.5mm feet (for flat dishes and plates). Strainers are supplied sterile, individually wrapped, in convenient blister packs, with one free reducing adapter. 

The advanced frame/housing design offers a unique geometry ensure aseptic handling and eliminate the contamination risks associated with fingers contacting the filtered suspension, mesh and/or interior surfaces. The enhanced performance and improved aeration, by eliminating the "air-lock" effect, also reduces processing time and prevents messy spillovers.

ReadyStrain™ is the first product of its kind, providing all the necessary parts for filtration of cell suspensions and combining them into a single, preassembled, ready to use, sterile packet. The kits eliminate the contamination risks associated with opening multiple packages, and assembling separate pieces. The individual sterile packets mean researchers are no longer forced to expose entire bags of 50mL tubes and corresponding caps to perform one filtration. Instead they can open a single ReadyStrain packet directly under the hood.
  

• Kit Includes: strainer, 50 mL tube & cap
• Opens directly under the hood reducing risk of contamination
• Pre-assembled - ready to use
• Guaranteed sterile - gamma
• Packaged in easy-open "tear-strip" bags

NEST Cell Culture Plates

• Sterilized by E-beam
• Vacuum Plasma tissue culture treatment
• Clear lot number for batch traceability
• Peel-to-open, individually wrapped
• Markings of well coordinates available for 12, 24, 48 96 and 384-well plates
• Non-Pyrogenic, DNase/RNase Free
• The package box of Nest cell culture plates can be used as reservoirs

Material
High clarity, 100% virgin polystyrene

Color-coded for easy identification 

Each box is secured with a rust-resistant nickel-plated clasp and hinge pin. Available with either cork or foam lining. Each slot is numbered to correspond to the slide inventory sheet on the inside cover of the box. Boxes stack securely on top of each other, and are available in blue, green, red, yellow, white, gray, or black. Measures 8.75 x 6.75 x 1.3".

• Hold 12 slides
• Integrated dual well reservoir can hold water when humidified storage is needed
• Made from heavy-duty cast acrylic
• Skid-proof rubber feet prevent sliding and facilitate sturdy stacking of trays
• Black or clear lid versions available

The new IBI AT-12 and ATC-12 Assay Staining Trays have been designed for immunohistochemistry slide staining. These 12-slide units have an integrated dual well reservoir to hold water below the slides when humidity is needed. The AT-12 has a black lid for light sensitive assays, and the ATC-12 has clear lid to allow visualization of the slides. These Assay Staining Trays also work great for Microscopy, Cytology, and Hematology.

Perfect for storage or transport 

These heavy-duty, two-piece microscope slide boxes store up to 25 standard slides (3 x 1" or 75 x 25mm). Available with either cork or foam lining. The boxes stack securely on top of each other, and are available in blue, green, red, yellow or white. Measures 5.5 x 3.5 x 1.24".

These disposable plastic cassettes hold tissue specimens during processing and embedding, as well as in storage. Molded from a special high-density polymer, they keep specimens safely submerged in liquid and are totally resistant to the chemical action of histological solvents. The efficient flow-through slots maximize fluid exchange and ensure proper drainage.

This new model differs by the special hinge holding the base and cover together. This hinge allows cassettes to be opened and closed as often as necessary. The cover can be removed and re-inserted easily without danger of specimen loss.

Available in 11 non-cytotoxic, non-metallic colors.

Each case contains 3 dispenser boxes of 500 cassettes.

HISTOETTE2 Embedding Cassette

Similar to M492 but specially designed to hold biopsy specimens during the embedding process, as well as in a storage cabinet. Anterior writing area is at a 45° angle to make the cassette more suitable to be used with certain types of cassette labeling instruments. Each case contains 3 dispenser boxes of 500 cassettes.

Embedding rings

Embedding rings are suitable for holding and identifying tissue sample blocks and fit well in microtome chuck adapters. The etched writing surface on the ring is marked with an identification number and placed on top of the sample block. Additional paraffin is poured into the base mold to cement the ring onto the tissue block. The embedding ring securely holds the tissue sample in the microtome chuck adapter for sectioning and then identifies the sample while in storage. Rings are available in different colors. Each case contains 4 dispenser boxes of 250 rings.

Molded from a special high density polymer, these cassettes keep specimens safely submerged in liquid and are totally resistant to the chemical action of histological solvents. The efficient flow-through slots maximize fluid exchange and ensure proper drainage. The one-piece integral lid eliminates the need for separate steel lids. The snaplatch and hinge-lock design of the UNISETTE prevent early separation of base and lid and allows for one-hand operation. Lids can be opened and closed as often as necessary and they always relock securely without danger of specimen loss. Large labeling areas are located on two sides of the cassettes. Anterior writing area is at a 35° angle.

M506 Series is similar to M505 but specially designed to hold biopsy specimens during the embedding process.

Available in non-cytotoxic, non-metallic colors. Each case contains 3 dispenser boxes of 500 cassettes.