General Tissue Culture Supplies

PR1MA Plates-Permeable Cell Culture Inserts

• Permeable Cell Culture Inserts mimic in vivo conditions
• Inserts center in wells, preventing membrane from touching sides and wicking between insert and side wall
• Membrane remains suspended above well bottom, allowing co-culture: grow one cell line inside insert, and another below in the well
• Ideal for drug transfer assays, transport studies, migration assays, and cell-cell interaction studies
• Transfer cultures to fresh media by moving the insert to a new plate
• 0.4 µm pore size is ideal for co-culture and drug transport studies
• 3.0 µm and 8.0 µm pore size are ideal for angiogenesis and chemotaxis experiments
• For more help with membrane selection, please contact CELLTREAT Scientific Products
• Non-Treated Plates sold separately; carrier plate is Tissue Culture Treated which encourages cell adhesion

Glass Bottom Plates

• Convenient live cell viewing without needing to transfer
• Optically clear borosilicate glass for exceptional viewing
• Applications include: cell culture and imaging, live cell observation and specialty microscopy such as confocal high resolution and contrast
• Constructed with noncytotoxic medical adhesive
• Round cover glass also available for use with glass bottom plates

Glass Bottom Dish

• Recessed, consistent borosilicate glass bottom is optically clear, and provides micro well ideal for cell culture and microscopy or imaging
• Non-cytotoxic medical adhesive does not inhibit cell growth
• 30mm Dish lid has molded spacers to allow gas exchange and a stacking ring for increased stability

Cover Glass

• High-quality, optically clear borosilicate glass cover slips are ideal for specialty microscopy and imaging
• Ideal for use with dishes and multiple well plates and anywhere a square cover glass is unsuitable
• Consistent flatness and scratch-free
• Resistant to organic solvents
• Packaged in a hinged plastic storage case
• 15mm Round Cover Glass can be used with a 24 well glass bottom plate (Part No. 229125)
• 20mm Round Cover Glass can be used with a 12 well plate or 30mm glass bottom dish (Part No. 229632)
• 25mm Round Cover Glass can be used with a 6 well glass bottom plate (Part No. 229107)
• Cover Glass is #1.5 thickness

Chambered Cell Culture Slides

• Cells grow directly on the slide without any need for future transfer
• Ideal for growing and viewing cells under a microscope on a standard 25mm x 75mm slide
• Uniform hydrophylic surface is ideal for consistent cell attachment
• Slides are labeled with numeric markings
• Packing trays can be used as incubation racks in CO2 incubators
• Adhesive free

PR1MA Centrifuge Tube Filter Systems

• Eliminate a sample transfer step by filtering directly into a 50mL tube
• Polyethersulfone (PES) membrane for high flow and low binding
• One Centrifuge Tube Stand included with each case of 50mL Centrifuge Tube Filter Systems
• Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable
• Centrifuge Tube Stand will hold one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL)

PR1MA Cell Strainers
 
PR1MA cell strainers from MIDSCI can be used for many applications including isolating cells and removing clumps or debris. They are compatible with standard 50 mL centrifuge tubes. The exclusive Pestle design improves manipulation of material in the strainer during operation.

• Polypropylene housing with nylon mesh filter eliminates the need to fabricate filters from gauze, paper, nylon mesh, or other materials
• Elongated lip on housing extends beyond edge of centrifuge tube enabling aseptic handling with forceps
• Extended gripping tab on housing for easy handling
• Available in three porosities:  40, 70 & 100 µm
• Color coded by mesh porosity for easy identification
• Packaged sterile (gamma irradiated) in individually wrapped peel-to-open paper/plastic wrapper or bulk packed in bags

• Expanded surface roller bottle increases surface area for cell culture
• Large knurled cap for easy grip
• Vented (HRBV) or non-vented cap (HRBNV) for open or closed systems
• Manufactured from medical grade virgin polystyrene
• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment
• Easy-to-read black printed graduations
• Packaged sterile in individual bags
• Vent caps (RBCAP) feature 0.22µm hydrophobic membrane

PR1MA Non-Treated Roller Bottles

• Available in two sizes: 1000 mL Vol with 490 cm² of surface area and 2000 mL Vol with 850 cm² of surface area, treated or non-treated versions
• Large knurled cap for easy grip
• Vented or non-vented cap for open or closed systems
• Manufactured from medical grade virgin polystyrene
• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment
• Easy-to-read black printed graduations
• Packaged sterile (gamma irradiated) in individual bags

Color-coded for easy identification 

Each box is secured with a rust-resistant nickel-plated clasp and hinge pin. Available with either cork or foam lining. Each slot is numbered to correspond to the slide inventory sheet on the inside cover of the box. Boxes stack securely on top of each other, and are available in blue, green, red, yellow, white, gray, or black. Measures 8.75 x 6.75 x 1.3".

NEST High Efficiency Erlenmeyer Flasks

• The body is made of polycarbonate (PC), which meets the USP Class 6 Standard, with high transparency, has strong impact resistance, oxidation resistance and can withstand a high temperature up to 121°C. Theres a scale made by injection molding on the flask · body to facilitate observing the filled liquid volume.
• The wide-mouth neck design is convenient for sampling and liquid filling.
• No DNA or RNA enzymes, no pyrogens
• Sterilized by E-Beam
• Packaged in sterile bags

Newly added 3 L wide-mouth high-efficiency Erlenmeyer flask

The bottom diameter of the 3-L wide-mouth high-efficiency Erlenmeyer flask is consistent with that of the 3 L high-efficiency Erlenmeyer flask, and its mouth diameter adopts the neck size of the 5 L high-efficiency Erlenmeyer flask, which not only saves space usage, but also increase oxygen flux, so as to increase the efficiency of cell culture.

Material

PC Bottle & HDPE Cap & Nylon Handle, Vent caps with 0.2um hydrophobic filters for gas exchange without contamination.

Application

Ideal for shaker culture applications.

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

NEST Transfer Cap for High Efficiency Erlenmeyer Flasks

Two-Way Liquid Transfer Cap
The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.

Multifunctional Liquid Transfer Cap
Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.

Inverted Liquid Transfer Cap
The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.

Application

It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.

Product Features

• Closed transfer reduces the risks of contamination during liquid transfer
• The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues
• A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported
• High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance
• Sterility level (SAL) is maintained at 10-6
• Endotoxin-free, and no ingredients of animal origin