• Quickly spins down droplets in PCR plates (less than 20 seconds)
• Accepts skirted, non-skirted, standard, and most microtiter PCR plates
• 50% smaller than traditional centrifuges
• Unique rotor design prevents sample spillage

The PR1MA™ Microplate Microcentrifuge is the first mini sized centrifuge with a swing out rotor for microplates. Centrifuging plates before PCR ensures all reactants are in the bottom of the wells for proper concentrations and improved yields.

The easily accessible rotor chamber includes two plate carriers that rest at a 75° angle. This allows plates to be inserted confidently without sealing tapes or caps. Upon closing the centrifuge lid, the rotor accelerates and the centrifugal force "swings" the plates into a horizontal position, concentrating any droplets on the plate walls to the well bottoms.

C2000-DWMP - This optional rotor for the PCRCENT Plate Centrifuge has carriers for deep well plates up to 35 mm tall.

MIDSCI bring you the new MultiGene™ Thermal Cycler, which guarantees to produce consistent and reliable results while making gradient technology affordable. This unit is extremely simple to program, compact in design and built to perform.

Features:

• Faster speeds - up to 30 min
• Gradient capability
• 6-segment block temperature control
• Independent block temperature selection capabilities
• Precision thermal control
• TM calculator
• PC Viewer
• No condensation after overnight cooling at 4°C
• Simple, user-friendly programming
• 3-yr warranty

The MultiGene™ OptiMax employs a new protocol optimization process improving on older gradient features. The OptiMax utilizes Flex™ technology that effectively separates the 96 well plate into 6 - (4 x 4) temperature regions that allow you to pick the temperatures you want to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures for you, you can choose any temperatures you want within a 24°C range.

The MultiGene™ OptiMax has a heating rate of 4°C /sec and a cooling rate of 3°C/sec. We have minimized overshooting and undershooting so that your reactions only see the temperatures they are supposed to see. This also leads to faster runs time.


New is the PC Viewer Option.

By downloading our software from our website and attaching a USB cable to your Windows PC to can view the temperature profiles real time as they are occurring to monitor your system.


Intuitive User Interface

The MultiGene™ OptiMax utilizes an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what our customers previously enjoyed in our earlier models. It can be used with lab gloves even if wet. We have improved the ramping speed and eliminated overshooting and undershooting which contributes to longer run times.


Fully Adjustable Heated Lid

The OptiMax's heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates. For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.

Product Specifications:

PR1MA™ Corbett Type PCR Tubes

• PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
• Tube Size is 0.1 mL
• 4 Tube Strip and 4 Cap Strip
• The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
• Reclosable bags minimize contamination risk and are convenient for opening and closing.
• For use in medical, pharmaceutical, food industry, and molecular biology.
• Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.

• 96-well, 8 x 12 format
• Holds 0.2ml PCR tubes, strip tubes, and plates (does not fit full skirted plates)
• Supplied with clear lift-off lids

• Available in 6-, 12-, 24-, 48-, and 96-well format
• Polystyrene, NOT Tissue Culture Treated (suspension culture)
• Sterile (gamma irradiated)

PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 µL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 µL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator   

IsoFreeze PCR and IsoFreeze PCR SBS are innovative, high performance, personal chiller racks. Using proven IsoFreeze temperature maintenance technology, the filled racks maintain a cool(refrigerator) environment on the bench or on the workstation platform. IsoFreeze gives a chilled and stable environment for PCR reagents, cells, enzymes or other temperature sensitive reagents.

Industry Standard format tubes, strips and 96 well plates, in both standard and low profile well shape, are accommodated and are efficiently cooled in PCR, QPCR and many other applications.

IsoFreeze PCR Racks are manufactured using polypropylene containing novel, temperature dependent color-change dye giving simple, at-a-glance, temperature indication for each well. There is a dramatic change to a contrasting color at 7C. IsoFreeze gel technology means the wells will stay cool for up to 4 hours (see charts below), depending on ambient temperature and other factors.

IsoFreeze PCR can keep your sample cool for over 4 hours!

IsoFreeze PCR SBS high performance is ideal for your automated systems!

IsoFreeze PCR and IsoFreeze PCR SBS available in 2 color change formats!

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy

Supplied as a ready-to-use 2X master mix, PR1MA qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. PR1MA Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.


*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

Deep Well Plates and Combs for KingFisher Systems 

FEATURING:

Ultra-flat surfaces
Alphanumeric grid reference
Raised Well Design - improved heat sealing and reduced cross contamination
Storage down to -80°C
Automation compliant footprint (SBS/ ANSI/SLAS specification)
Made from Virgin Polypropylene; no mold release agents or regrind
RNase, DNase, DNA, and Pyrogen Free Certification

MID-KF05US-9-N

Our 250 ul 96-well elution plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         350 ul
Working Volume:            250 ul
Volume with Mat:          250 ul
Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT

MID-KF10US-24-N

Our 5.0 mL 24-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification (of larger sample sizes, 5 mL) performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         10.0 mL
Working Volume:            5.0 mL
Volume with Mat:          5.0 mL
Matching silicone cap mats: MID-MATS-24-N

MID-KF22US-9-N

Our 2.0 mL 96-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems.  The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads.  Wells also have a conical V-shaped bottom to help concentrate precipitates.

Maximum Volume:         2.0mL
Working Volume:            1.0mL
Volume with Mat:          1.0mL

PR1MA Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low copy and long PCR
• Enzyme of choice for TA cloning

PR1MA Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number and long PCR.

The 3' -5' exonuclease activity of the proofreading enzyme produces fragments suitable for TA cloning. PR1MA Taq Plus is provided with a 5X buffer with dNTPs or in a convenient one tube 2X Master Mix.

PR1MA Taq Start Taq DNA Polymerase

PR1MA Taq Plus Master Mix, 2X Concentration

The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.

• Flexibility:  Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.
• Intelligent workflow: The user interface is a simple touch screen assay setup.  Protocols can be saved to make assay set up easy and reproducible.
• Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.
• Connectivity: An external PC is not required to run the instrument.
• Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes PR1MA Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.
*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.