• Perfect box for every blot
• Reduce antibody usage
• Hinged Lid minimizes evaporation
• For halves, wholes, strips, or minis

No matter what size your Western blot is, there's a box that fits it perfectly. With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. Additionally, all corners are formed at 90° angles and the bottom surface is uniformly flat, further reducing consumption of costly reagents and antibodies (for example, popular mini-gels require only about 4mL).

At a price that makes them truly disposable, all sizes are engineered from crystal clear polystyrene. The exception is our opaque black boxes (except WBB7BK and WBB15BK), which protect light sensitive samples. All sizes are also equipped with an easy-to-open hinged lid that reduces evaporation.

Ordering Information

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

Western Blot 2.0 with PURITY™

The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.

Benefits:

• 1 simple protocol which makes almost all optimization unnecessary.
• Increased sensitivity and reproducibility of blots.
• Dramatically reduces the number of steps and time required for Western blots.
• Drastically decreases background phenomena.
• Highly reliable.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.