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REPLACEMENT GD COLUMNS & COLLECTION TUBES - 100 PACK
- For use with leftover reagents from IBI Genomic DNA Kits (Tissue, Blood & Cultured Cells) or competitive products
- Sample Size: 300 mL fresh whole blood/107 animal cultured cells
- Elution Volume: 50 to 200 µL
- Binding Capacity: Up to 10 µg
The Replacement Genomic DNA Columns & Collection Tubes are for use with leftover reagents from IBI Genomic DNA Kits including the following item numbers: IB47201, IB47202, IB47211, IB47221, IB47222, IB47231, IB47241, IB47242, IB47281, IB47282, IB47291, and IB47292.
The columns and collection tubes can also be used with comparable, competitive Genomic DNA Kit reagents that utilize a lysis, bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
Isolation of micro RNA (miRNA) from Tissue or Cultured Cells
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Sample Types: |
100mg tissue or 1 x 10 cultured cells |
Format: |
Spin column |
Operation Time: |
30 minutes |
Isolation of micro RNA (miRNA) from Tissue or Cultured Cells
The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA.
In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.
Sample Types: |
100mg tissue or 1 x 10 cultured cells |
Format: |
Spin column |
Operation Time: |
30 minutes |
REPLACEMENT PD COLUMNS AND COLLECTION TUBES -100 PACK
- For use with leftover reagents from IBI Plasmid Kits or competitive products
- Sample Size: 1 -4 mL bacterial culture
- Elution Volume: 50 to 100 µL
- Binding Capacity: Up to 30 µg
The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.
The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
SPECIFICATIONS
Sample Size: 1 - 4 mL bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 -30 µg for high copy and 3 -10 µg for low copy.
Elution volume: 50 µL to 100 µL
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.
REPLACEMENT DF COLUMNS AND COLLECTION TUBES - 100 PACK
- For use with leftover reagents from IBI PCR/Gel Extraction Kits or competitive products
- Sample Size: 300 mg agarose gel/100 µL PCR product
- Elution Volume: 20 to 50 µL
- Binding Capacity: Up to 10 µg
The Replacement DF Columns and Collection Tubes are for use with leftover reagents from IBI PCR/Gel Extraction Kits including the following item numbers: IB47020 and IB47030.
The replacement spin columns and collection tubes can also be used with comparable, competitive PCR/Gel Extraction Kit reagents that utilize a bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
SPECIFICATIONS
Sample Size: 300 mg of agarose gel or 100 µL of PCR product
Format: Spin column or vacuum manifold
Expected yield: 90% for Gel extraction and 95% for PCR Clean-up
Elution volume: 20 µL to 50 µL
Operation time: 20 minutes
Purified DNA is ready for use in Fluorescent or Radioactive sequencing, ligation, PCR, restriction enzyme digestion, and DNA labeling.
High Throughput PCR Clean-Up
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Sample Size: |
Up to 50µl of PCR product |
Format: |
96-Well Plates |
Operation: |
Centrifuge/Vacuum manifold |
Binding Capacity: |
10ug per well |
DNA Size: |
50bp-10kb |
Recovery: |
80-90% for Gel Extraction |
Operation Time: |
30 min for PCR Clean-Up |
High Throughput PCR Clean-Up
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Sample Size: |
Up to 50µl of PCR product |
Format: |
96-Well Plates |
Operation: |
Centrifuge/Vacuum manifold |
Binding Capacity: |
10ug per well |
DNA Size: |
50bp-10kb |
Recovery: |
80-90% for Gel Extraction |
Operation Time: |
30 min for PCR Clean-Up |
fficient Gel Extraction and PCR Clean-up
The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Sample: |
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction |
Format: |
Spin columns |
Operation: |
Centrifuge/Vacuum Manifold |
Binding Capacity: |
10µg DNA |
Expectant Yield: |
80-90% for gel extraction
90-95% for PCR clean-up |
| Operational Time: | 15min. for PCR clean-up/20min. for gel extraction |
Applications: |
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations |
fficient Gel Extraction and PCR Clean-up
The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Sample: |
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction |
Format: |
Spin columns |
Operation: |
Centrifuge/Vacuum Manifold |
Binding Capacity: |
10µg DNA |
Expectant Yield: |
80-90% for gel extraction
90-95% for PCR clean-up |
| Operational Time: | 15min. for PCR clean-up/20min. for gel extraction |
Applications: |
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations |
- Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
- Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
- Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
- Elution Volume: 50 200µl (genomic DNA)/25 50µl (total RNA)
- Format: genomic DNA spin column and total RNA spin column
IBI DNA/RNA/Protein Extraction Kits
The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.
DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.
The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.
Specifications
Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50 200ul for Genomic DNA / 25 50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.
- Sample: Cultured cells
- Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
- Format: Scalable DNA precipitation method
- Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods
The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 μg with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.
gPURE DNA Isolation Kit |
IB47430 |
IB47431 |
IB47432 |
Number of Cells Processed Per Kit |
2 x 10 |
6 x 10 |
6 x 10 |
Cell Lysis Buffer |
3 mL |
100 mL |
1000 mL |
Protein Removal Buffer |
1 mL |
40 mL |
400 mL |
DNA Hydration Buffer |
1 mL |
50 mL |
500 mL |
RNase A (10 mg/mL) |
25 µL |
550 µL |
5 mL |
Cell Number |
0.5-1 x 10 |
3-5 x 10 |
3.5 x 10 |
Tube Size |
1.5 mL |
1.5 mL |
15 mL |
Cell Lysis Buffer |
150 µL |
600 µL |
6 mL |
RNase A (10 mg/mL) |
1 µL |
3 µL |
30 µL |
Protein Removal Buffer |
50 µL |
200 µL |
2 mL |
Isopropanol |
150 µL |
600 µL |
6 mL |
70% Ethanol |
150 µL |
600 µL |
6 mL |
DNA Hydration Buffer |
50 µL |
100 µL |
200 µL |
- Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
- Yield: 5-30 µg
- Format: Spin column
- Operation time: Within 20 minutes
Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.
Quality Control
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
Kit |
IB47300 |
IB47301 |
IB47302 |
IB47303 |
RB Buffer |
2 mL |
30 mL |
60 mL |
130 mL |
W1 Buffer |
2 mL |
30 mL |
50 mL |
130 mL |
Wash Buffer* |
1 mL |
12.5 mL |
25 mL |
50 mL x 2 |
RNase-free H2O |
1 mL |
6 mL |
15 mL |
30 mL |
RB Column |
4 pcs |
50 pcs |
100 pcs |
300 pcs |
Filter Column |
4 pcs |
50 pcs |
100 pcs |
300 pcs |
2 mL Collection Tubes |
8 pcs |
100 pcs |
200 pcs |
600 pcs |
Micropestle |
4 pcs |
50 pcs |
100 pcs |
300 pcs |
*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.
Caution
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.
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