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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Barcoded versions available upon special order.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free
- PR-PCR2196F has a frosted surface for optimal fluorescence detection. A frosted surface reduces background noise that can interfere with quantitative real-time PCR (qPCR).
PR1MA™ MyVolt Touch Power Supply
- Touch screen control
- Bright color display
- Dual orientation, auto rotating display
The PR1MA™ MyVolt™ Touch is a work horse electrophoresis
power supply, ideal for efficient separation of nucleic acids and proteins as
well as basic western blotting applications. Four pairs of output jacks are
wired in parallel for connecting multiple electrophoresis tanks. A color touch
screen allows quick setting and monitoring of all run conditions, including run
time, voltage, and current. Users can
select “Constant Current” or “Constant Voltage” during an electrophoresis run,
keeping the chosen parameter fixed as resistance changes. The dual orientation
design with rotating display allows the MyVolt™ Touch to be installed in the
most convenient location, on a shelf or bench, saving valuable space.
The PR1MA™ MyVolt™ Touch features a logical user interface. Setting up an electrophoresis run couldn’t be easier; simply touch the parameter to select it, press the “up” and “down” arrows to adjust the value, and then touch “Start”. All parameters are always displayed, with no need to toggle between Time, Voltage or Amperage. In addition, the run status is always displayed.
Output Settings | Constant voltage or constant current |
Voltage Output | 10-300 V (1 V increments) |
Current Output | 1-400 mA (1 mA increments) |
Max Wattage | 100 W |
Timer | 1 min. to 20 hours or continuous |
Output Terminals | 4 pairs (wired in parallel) |
Safety | No load detection, overload detection with power limitying function |
Display | 2 x 2 in. color touchscreen |
Dimensions | 8.5 x 6.3 x 4.0 inches |
Weight | 2 lbs |
Electrical* | 100 V to 230 V, 50-60 Hz (Universal Input) |
Warranty | 2 years |
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Barcoded versions available upon special order.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
- PR1MA™ Individual PCR tubes are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
Mystaire® PCR Prep Stations
They are compact laminar flow enclosures that reduce the risk of sample contamination while performing polymerase chain reaction experiments.
Airborne contaminants are prevented from entering into the work zone through constant positive pressure HEPA filtered air. Each PCR prep station is equipped with a built-in 254 nanometer shortwave lamp to effectively decontaminate the work area between amplifications. Safety interlocks are included on all PCR prep stations to prevent operator exposure to potentially harmful UV radiation.
MY-PCR prep stations are bench-top personal clean zones allowing the operator to amplify RNA and DNA while minimizing the potential for background from airborne contaminants.
Note: MY-PCR and FE-PCR are not biological safety cabinets. They are designed to provide process protection only from potential airborne contaminants found in the laboratory space.
MY-PCR WORKSTATIONS FEATURES
- Class 100 vertical laminar flow air
- 15-Minute UV light timer
- Clear polycarbonate construction with chemically impervious polypropylene work surface
- UV shelf with integrated pipette holder
- Electrical cord pass-through port
Sash position switch and overlap to prevent UV light exposure
Specs |
MY-PCR24 |
MY-PCR32 |
MY-PCR48 |
Width |
24" |
32" |
48" |
Depth |
24" |
||
Height |
30" |
||
Noise Level |
<45 DBA |
<49 DBA |
<56 DBA |
Voltage |
110 V or 220 V |
||
Power |
200 Watts |
400 Watts |
|
UV Light |
15 W/254 mm |
15 W/254 mm (2) |
|
Blower |
Sparkless Brushless |
||
UV Light Timer |
0-15 min. |
||
Safety Interlock |
Yes |
||
Air Velocity |
60 LFM |
HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.
- PR1MA™ Sealing Mats are made of silicone and the dimensions are 4.67 in. (L) x 2.90 in. (W).
- Pierceable
- Designed for an evaporation-resistant seal to minimize sample loss.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, DNA, and PCR inhibitors.
- Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR
- Improved template affinity and solubility for higher enzyme activity and greater yields
- Proprietary buffer optimized for a variety of assay conditions
- Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer
PR1MA Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.
The polymerase is supplied with a 5X buffer containing MgCl2 and proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.
PR1MA™ Microplate Microcentrifuge
- Quickly spins down droplets in PCR plates (less than 20 seconds)
- Accepts skirted, non-skirted, standard, and most microtiter PCR plates
- 50% smaller than traditional centrifuges
- Unique rotor design prevents sample spillage
The PR1MA™ Microplate Microcentrifuge is the first mini sized centrifuge with a swing out rotor for microplates. Centrifuging plates before PCR ensures all reactants are in the bottom of the wells for proper concentrations and improved yields.
The easily accessible rotor chamber includes two plate carriers that rest at a 75° angle. This allows plates to be inserted confidently without sealing tapes or caps. Upon closing the centrifuge lid, the rotor accelerates and the centrifugal force "swings" the plates into a horizontal position, concentrating any droplets on the plate walls to the well bottoms.
Specifications
Capacity |
2 PCR Plates (4 if stackable) |
Rotor Speed |
2,100 rpm |
G Force |
400 x g |
Rotor |
Swing Out |
Dimensions (W x D x H) |
9.2 x 10.2 x 7.75 in. | 23 x 26 x 19.7 cm |
Weight |
9 lbs. | 4.1 kg |
Electrical |
120V or 230V, 50-60 Hz |
Accessories
C2000-DWMP - This optional rotor for the PCRCENT Plate Centrifuge has carriers for deep well plates up to 35 mm tall.