MIDSCI bring you the new MultiGene™ Thermal Cycler, which guarantees to produce consistent and reliable results while making gradient technology affordable. This unit is extremely simple to program, compact in design and built to perform.

Features:

• Faster speeds - up to 30 min
• Gradient capability
• 6-segment block temperature control
• Independent block temperature selection capabilities
• Precision thermal control
• TM calculator
• PC Viewer
• No condensation after overnight cooling at 4°C
• Simple, user-friendly programming
• 3-yr warranty

The MultiGene™ OptiMax employs a new protocol optimization process improving on older gradient features. The OptiMax utilizes Flex™ technology that effectively separates the 96 well plate into 6 - (4 x 4) temperature regions that allow you to pick the temperatures you want to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures for you, you can choose any temperatures you want within a 24°C range.

The MultiGene™ OptiMax has a heating rate of 4°C /sec and a cooling rate of 3°C/sec. We have minimized overshooting and undershooting so that your reactions only see the temperatures they are supposed to see. This also leads to faster runs time.


New is the PC Viewer Option.

By downloading our software from our website and attaching a USB cable to your Windows PC to can view the temperature profiles real time as they are occurring to monitor your system.


Intuitive User Interface

The MultiGene™ OptiMax utilizes an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what our customers previously enjoyed in our earlier models. It can be used with lab gloves even if wet. We have improved the ramping speed and eliminated overshooting and undershooting which contributes to longer run times.


Fully Adjustable Heated Lid

The OptiMax's heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates. For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.

Product Specifications:

Designed for laboratories with medium to high throughput needs, the TC9639 Thermal Cycler provides fast, reliable results. Featuring a large, touch screen interface, a next-generation program wizard and a unique multi-format block, this unit makes it easier than ever to get up and running.

A large, full color touch screen serves as the operations center where all functions are accessed. Starting at the "Home" screen, simply touch an icon to view the last run, enter the program library or use the program wizard to quickly enter a new protocol.

Designed with extensive user feedback, the program wizard quickly and easily guides users through protocol set-up, all in a single screen. Time and temperature increments can be built into programs as well as slower ramp rates. Storage capabilities are unlimited; programs can be stored in public folders, password protected folders or on a flash drive.

The fully adjustable heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Heating and cooling of the block are precisely and accurately controlled within exceptionally tight tolerances by a proprietary algorithm that simulates sample temperature.

With the multi-format block, there is no need for the expense of multiple interchangeable blocks. The TC 9639 has the flexibility to accept 96 x 0.2ml tubes, a 96 well plate or 39 x 0.5ml tubes, all in the same sample block. An in-situ adapter is available for use with slides. A 384 well version is available.

• Gradient capabilities- ideal for protocol optimization
• Intuitive touch screen control- Works even when wearing gloves!
• Upgraded software- Even easier to use than before!
• No need for interchangeable blocks- multi-format accepts:
• 0.2ml tubes or strips (96)
• 0.5ml tubes (39)
• 96 well PCR plate

PRIME G (gradient) Thermal Cycler

• Colour touchscreen for fast program setup
• Small space-saving footprint
• Temp Range 4°C to 100°C
• Fast ramp rate of up to 3.0°C/sec
• Larger block sizes; 48 x 0.2ml, 30 x 0.5ml and half a 96 well plate in horizontal format.
• Gradient can be applied across a Temp Range of 30°C to 80°C
• Maximum Gradient 30°C
• Gradient calculator function
• Data transfer via USB
• 4 year warranty

The 3 PRIME G is a small gradient thermal cycler that builds on all the features of the 3 PRIME X instrument. The 48 well block format offers eight columns for annealing temperature optimisation and six rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over 30oC between temperatures 30°C to 80°C. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions.

Technical Specification

Key benefits
Speed

• 40 cycles in 40 minutes before fast optimization.
  
• 40 cycles in 15 minutes when optimized.
  
• Genotype in 4 minutes with over 99% accuracy.
  
• Set up a 4plex reaction in only 4 mouse clicks.
  
  Confidence
  
• MIQE compliant - always consistent, always compliant.
  
• Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
  
• 400 specific qPCR reagent kits available.
  
  
  Performance
  
• Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
  
• Compile data from multiple experiments. Pool plates and results across labs and instruments.
  
• Record every well, with every filter, in every cycle. Never miss a data point.
  
• Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighboring wells or maximizing the wide dynamic range. Trust results are correct. 
  
• All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.
  
  Value
  
• No other qPCR system offers so much performance for such a low price.
  
• Available as a complete system - Techne instrument, plates, seals and reagents.
  
• Alternatively, is chemically open platform - use your preferred supplier reagents.
  
• Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  
• Everything as standard - no add-ons, HRM as standard, unlimited software license. 
  
• Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving.
  
  Sensitivity
  
• ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  
• HRM - Identify Class IV SNP >99.9% of the time
  
• Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.

   

  The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.
  
  High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimized 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a
  unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.
  
  The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.
  
  Technical Specification

  High Resolution Melt	Yes
  Volume per well	Validated for 5 to 20 ul
  Detection sensitivity	1 copy
  Temp uniformity	+- 0.1 °C within 1 sec
  Temp stability	+- 0.1 °C
  Temperature range	30 to 100 °C
  Average ramp rate	5.5 °C sec
  Thermal system	Hollow silver block peltier-based with conductive fluid
  Block format	48-well block
  Consumables	48-well custom plates and optical adhesive seals
  Optical system	Dual LED excitation (452 to 486 nm and 542 to 582 nm)
  Camera	CCD camera
  Filters	4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
  Calibrated dyes	SYBR FAM HEX ROX Cy5
  Additional dyes	All dyes within range compatible with no additional calibration
  Passive reference dyes	Use of ROX is supported but optional
  Data collection	Data always collected in all four filters for all wells
  Plate setup	Plate setup for data analysis can be altered after run completes
  Melt curve	Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
  PCR cycle time	40 cycles in less than 40 minutes
  PCR cycle time (FAST optimized)	40 cycles in less than 20 minutes
  Dynamic range	Greater than 9 logs
  Calibration	Not required
  Installation	Plug and play design
  Precision	Discriminates 5000 and 10000 template copies with 99 percent confidence
  Warranty	1 year (parts and labor included)
  Voltage	120 to 240 V
  Frequency	50 and 60 Hz
  Nominal current draw	8A
  Peak power	500W (typical power is 180W)
  Software	Multiple license software included at no additional cost
  Chemistry	All chemistries supported
  Applications	Absolute Quantification and Relative Quantification
  Applications	Allelic Discrimination and High Resolution Melt
  Dimensions closed (WxDxH)	34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
  Dimensions open (WxDxH)	34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
  Weight	13.6 Kg (30 lb)

   

   

  Ordering information

  Part number	Description
  PRIMEPRO48	Prime Pro 48 Real Time PCR System, includes Accessories and Software (Pro and ProControl). PC not supplied
  PROPLATE48	Pack of 50 Real Time PCR Plates, 48-wells
  PROSEAL48	Pack of 50 Real Time PCR Plate seals

PRIME Base Thermal Cycler

• Colour touchscreen for fast program setup.
• Temp Range 4°C to 100°C
• Fast ramp rate of up to 3.4°C/sec
• Can be upgraded for gradient cycling
• Data transfer via USB
• 4 year warranty

This full sized thermal cycler delivers both high performance and high throughput to provide maximum flexibility when processing a large number of samples in parallel. 


User friendly programming is achieved via a colour touchscreen and intuitive software that is standardised across the entire Techne thermal cycler range. A USB port enables the transfer of programmes between instruments and temperature logs to your PC. A real time graphical display provides an instant visualisation of the program status. 

Gradient cycling can be achieved directly using the PRIME G instrument or by upgrading the PRIME BASE unit. This enables the laboratory to manage changing cycling requirements in a cost effective manner.

The Techne PRIME cycler range is one of the most affordable full sized thermal cyclers on the market and is backed up by a 4 year warranty.

Technical Specification

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5 Prime G gradient thermal cycler

PRIME G (gradient) Thermal Cycler

• Colour touchscreen for fast program setup.
• Versatile block format
• Temperature range 4°C to 100°C
• Fast ramp rate of up to 3.4°C/sec
• Gradient can be applied across a temperature range 30°C to 80°C
• Maximum Gradient 30°C
• Gradient calculator function
• Data transfer via USB
• 4 year warranty

The PRIME G is a gradient enabled thermal cycler with all the features of the PRIME BASE unit. The wide linear gradient with a range of 30°C allows annealing temperatures to be optimised in one experiment. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions.

Technical Specification

• Fast ramping - up to 5°C/second
• Intuitive graphical programming
• Lightweight and compact
• Interchangeable blocks - 24 x 0.2 ml and 18 x 0.5 ml
• Economical - half the price of a full size unit
• 18 month warranty

• "Mini" sized, 9 x 7 inch footprint
• Capacity for 32 x 0.2 mL or 4 PCR Strips
• Intuitive touch screen control

"Mini sized" doesn't have to mean a sacrifice on features and performance. PR1MA's™ LD032 Mini Thermal Cycler offers advanced programming features, easy to use software and fast, reliable cycling but with a smaller block and lower price point.

Designed for lower throughput needs, the LD032 Mini Thermal Cycler has a capacity for 32 x 0.2 mL tubes. The wells are arranged in a 4 x 8 format, allowing the block to accept strips of 8 as well as individual tubes. The block heats and cools very quickly - at rates as fast as 5°C/second. A proprietary algorithm controls block temperature, bringing samples quickly to the target temperature without any over- or under-shoot. Excellent block uniformity ensures consistency sample to sample.

Operation of the thermal cycler is controlled from the large, full color, touch screen. Icon driven menus allow users to get running quickly. Programming is extremely simple and includes options for time and temperature increment/decrements, adjustable ramp rates, and an auto pause and restart functions. More than 100 typical programs can be stored in memory and easily recalled. After a run, samples can be held at 4°C. An on-screen report is also available at the completion of a run.

The heated lid is self-adjusting - the spring loaded platen in the lid comes firmly into contact with the tops of the tubes to prevent sample evaporation and condensation. There are no knobs to turn or levers to adjust. The temperature of the heated lid can be changed, or the heating function of the lid may be turned off.

With a footprint less than that of the average laboratory notebook, the LD032 Mini Thermal Cycler will fit almost anywhere. Its reduced capacity block and economical price make the TC-32 Mini Thermal Cycler the perfect choice for lower throughput laboratories and educational facilities.

Specifications