PR1MA High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

PR1MA qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

PR1MA qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and reverse transcriptase. Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  PR1MA Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95°C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

qMAX™ Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

**New Name, Same Great Product!**

PR1MA SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details

Protocol:  Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration:  0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage:  SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production:  SmartCheck™ ladders are produced from proprietary plasmids, digested to completion.  A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.
 
 

Item	Description	Volume
PR4005-100	PR1MA SmartCheck™ 50bp DNA Ladder	500 µL / 100 Lanes
PR4005-500	PR1MA SmartCheck™ 50bp DNA Ladder	5 x 500 µL / 500 Lanes
PR4010-100	PR1MA SmartCheck™ 100bp DNA Ladder	500 µL / 100 Lanes
PR4010-500	PR1MA SmartCheck™ 100bp DNA Ladder	5 x 500µL / 500 Lanes
PR4100-100	PR1MA SmartCheck™ 1kb DNA Ladder	500 µL / 100 Lanes
PR4100-500	PR1MA SmartCheck™ 1kb DNA Ladder	5 x 500 µL / 500 Lanes

PR1MA Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

Item	Description	Volume
PR1300-MG-S	PR1MA 1 Hour Mammalian Genotyping Kit	8 Reactions (Sample)
PR1300-MG-80	PR1MA 1 Hour Mammalian Genotyping Kit	80 Reactions
PR1300-MG-400	PR1MA 1 Hour Mammalian Genotyping Kit	400 Reactions
PR1300-MG-800	PR1MA 1 Hour Mammalian Genotyping Kit	800 Reactions

 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

Item	Description	Volume
PR1301-HSR-400	PR1MA Genotyping Hot Start Master Mix, 2X Conc., Red Dye	400 Reactions

 
 

IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

IB42300	PCR Grade Water,nuclease free,1.8 mL vial
IB42301	PCR Grade Water,nuclease free,(20)x 1.8 mL vial
IB42302	PCR Grade Water,nuclease free,(50) x 1.8 mL vial
IB42303	PCR Grade Water,nuclease free,(100) x 1.8 mL vial

Pfu 2x Master Mix 
Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

1. Pfu 2x master mix
2. 5x Magic Enhancer

Store all contents at -20 °C.

1x Master Mix Composition 
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.

Protocol
1. Prepare a reaction mix according to the following table:
  

PCR reaction set up:
Template DNA	1-50 ng
Forward primer (5 µM)	1.0µl
Reverse primer (5 µM)	1.0µl
Pfu 2x master mix	10.0µl
5x Magic Enhancer (optional)	4.0µl
HO up to	20.0µl

2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer's instructions.
4. A typical PCR cycling program is outlined in the following table.

 

PCR cycling conditions:
Steps	Temp.	Time	Cycles
Initial Denaturation	95 °C	3 min	1
Denaturation	95 °C	30 sec	25-40
Annealing	50-66 °C	30 sec
Extension	72 °C	1 min/kb
Final Extension	72 °C	5 min	1
Hold	4-12 °C	

5. Place the PCR tubes in the thermal cycler and start the cycling program.

6. Analyze 5 µl of PCR products by agarose gel electrophoresis. 

Pfu DNA Polymerase 
Pfu DNA polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). This product is supplied with the unique Intact Genomics 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM, and 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%. The physical purity of this enzyme is 98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

1. Pfu DNA Polymerase
2. 10x PCR Buffer with Mg²+
3. 5x Magic Enhancer
4. 10 mM dNTP (Cat. # 3312d, 3314d only)

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C.

10X PCR Buffer with Mg2+ 
100 mM Tris-HCl pH 9.0, 15 mM MgCl, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630

Unit Definition 
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 °C.

Protocol
1. Thaw 10x PCR Buffer, dNTP mix, primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use. 
2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.

 

PCR reaction set up:
Template DNA	xµl (0.01-0.5µMg)
10x PCR Buffer	10.0µl
dNTP (10 mM)	2.0µl
Forward Primer	xµl (0.1- 0.5µMM)
Reverse Primer	xµl (0.1- 0.5µMM)
5x Magic Enhancer (optional)	20µl
Pfu DNA Polymerase (5 U/µl)	0.5µl
H2O up to	100.0µl

3. Mix the reaction mixture thoroughly.
4. Add template DNA to the individual PCR tubes containing the reaction mixture. 
5. Program the thermal cycler according to the manufacturerâs instructions. A typical PCR cycling program is outlined in the following table.

PCR cycling conditions:
Steps	Temp.	Time	Cycles
Initial denaturation	95 °C	3-5 min	1
Denaturation	94 °C	30-60 sec	25-35
Annealing	52-66 °C	30-60 sec
Extension	72-74 °C	1-2 min
Final extension	72-74 °C	10 min	1
Hold	4-12 °C	â

6. Place the PCR tubes in the thermal cycler and start the cycling program.

Cas9 Nuclease 
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence

Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is â¥98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Source 
E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).

Contents & Storage

1. Cas9 Nuclease
2. 10x Cas9 Nuclease Reaction Buffer

Store Cas9 Nuclease and Buffer at -20 °C

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

1x Cas9 Reaction Buffer 
20 mM HEPES, 100 mM NaCl, 5 mM MgCl, 0.1 mM EDTA, pH 6.5 @ 25 °C

Functional Testing
Cas9 Nuclease functional testing was done by in vitro DNA cleavage assay with the following protocol which gives more than 95% digestion of the substrate DNA as determined by agarose gel electrophoresis.
1) Set up 30 µl reaction in a microcentrifuge tube on ice with the following combinations.

Target DNA	x µl (100ng)
sgRNA	x µl (4000ng)
10x Cas9 Reaction Buffer	3.0 µl
Cas9 Nuclease	1.0 µl (160ng)
Add H2O up to	30.0 µl

  

2) Gently mix the reaction mixture and centrifuge briefly.
3) Incubate at 37 °C for 60 min. 
4) Add 1 µl RNase (4 mg/ml) 
5) Incubate at 37 °C for 20 min.
6) Run 0.7 to1% agarose TBE gel.

HS Taq Polymerase Master Mix BLUE is a ready to use 2 X master mix. Simply add primers, template and water to successfully carry out primer extensions. The master mix is available in 100, 500, 1000, and 2500 reaction sizes and is available with two different buffer options (HS Buffer 1 and HS Buffer 2)

HS Taq Polymerase is a modified form of Bullseye Taq, activated by heat treatment. This results in higher specificity and greater yields when compared to standard Taq. The added benefit of the HS Master Mix Blue is that it can be loaded directly onto the gel as there is no need to use separate loading dyes for subsequent electrophoresis and visualization. This cuts down on the chance of contaminating the component stocks and leads to better reproducibility.

Composition of 2 X HS Master Mix Blue with Buffer 1
Tris-HCl, pH 8.5, (NH4)2SO4,3 mM, MgCl2, .2 % Tween 20, .4 mM dNTPs, .2 units/uL HS Taq Polymerase, Inert Blue Dye, Stabilizer

Composition of 2 X HS Master Mix Blue with Buffer 2
Tris-Hcl, pH 8.5, Balanced KCl/(NH4)2SO4,3 mM, MgCl2, .2 % Tween 20, .4 mM dNTPD, .2 units/uL HS Taq Polymerase, Inert Blue Dye, Stabilizer

New and Improved! Ideal for General PCR, Genomic analysis and TA cloning!

Bulleye Taq DNA Polymerase is the most popular thermostable enzyme used in DNA amplification experiments. This high performance Taq DNA polymerase is specifically purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the best-value DNA polymerases available.

• Robust performance
• Leaves 3' A overhang
• Stable at all storage temperatures
• Ideal for general PCR, genomic analysis and TA cloning
• This product is not recommended for work with neo-primers

Order#	Description	Quantity	Rxn
BETAQ-1000	Taq DNA Polymerase	1x200µl	1000U
BETAQ-5000	Taq DNA Polymerase	5x200µl	5000U
BETAQ-10000	Taq DNA Polymerase	10x200µl	10,000U

Storage: -20°C

Quality Control:
Taq DNA Polymerase is highly purified, free of contaminating endonucleases, exonucleases and nicking activity. For endonuclease assay, 1µg of Lamda/Hind III DNA is incubated with 20 units of enzyme in assay buffer at 75oC for 16 hours with no visible contaminating activity observed. Also, every lot is tested for its performance consistency.

Unit Definition:
One unit incorporates 10nmoles of 4 radioactive labeled dNTPs into acid-insoluble material in 30 minutes at 74°C.

Storage Buffer:
5 units/µl in 50mM Tris-HCl (pH8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% TritonX-100, and 0.5% NP-40.
10x Reaction Buffer 100mM KCl, 100mM Tris HCl (pH9.0), 80mM (NH4)2SO4, and 1.0% Triton X-100. (Mg++free): is optimized for use with 200µM dNTPs.

Magnesium Chloride:
25mM MgCl2: In general, 1.5mM MgCl2 is recommended; this may vary with different conditions and primer sets.

Please give us a call for a sample.

For laboratory research only. Not for clinical applications.

 

• Pre-optimized ready-to-use PCR reagents
• Less pipetting to avoid contamination
• Quick and easy to set up
• Direct loading for electrophoresis
• Reproducible results

Users only need to add templates and primers, and water if needed. Extra 25mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.

Storage: 4°C for up to one month, or -20°C for long term storage.

Magnesium Chloride: In general, 1.5mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:

Final MgCl2 concentration	Additional 25mM MgCl2 per 50µl reaction
1.5mM	0 µl
2.0 mM	1.0 µl
2.5mM	2.0 µl

Directions for use: For a 50µl reaction, use 25µl of the Taq Master Mix, add template, primers, and water to a final volume of 50µl. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minutes/kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.

1x Composition: 10mM KCl, 20mM Tris HCl (pH9.0), 16mM (NH4)2SO4, 0.1% Triton X-100, 1.5mM MgCl2, 200mM dNTPs, 2.5units/25ul of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.