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The Azure Cielo Real-Time PCR system provide accuracy and sensitivity needed for research, with intuitive touch screen software.
Product Features- Flexibility: Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range.
- Intelligent workflow: The user interface is a simple touch screen assay setup. Protocols can be saved to make assay set up easy and reproducible.
- Reliability: Cielo's optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1,000 qPCR experiments, giving you confidence in the reliability of the instrument.
- Connectivity: An external PC is not required to run the instrument.
- Data Transfer: This can be performed via Ethernet, Wi-Fi or USB.
Operation - A standalone qPCR system with
10.3” touchscreen interface
Optics - 3 or 6 dye channel filters with LED based
excitation for multiplexing
Speed - Fiber optic detection system scans 16 wells simultaneously
Durability - Tested
reliability for over 1000 continuous runs
Connectivity - Easily
export data via Wi-Fi, ethernet, USB or LAN
Capacity - 96-well format for semi-skirted or un-skirted plates and
tubes
Thermal Performance - Marlow
Peltier based block with 12 temperature gradient zones, ramp rates up to
6.0°C/sec
Chemistry - Supports
reaction volumes between 10 μl–150 μl, open source chemistry, including FAST
~20 minute run-times
Sensitivity - Detect
2-fold differences in target quantities in singleplex reactions and up to 1
copy of amplified DNA, 10 logs of dynamic range
Azure
Cielo 3 AIQ030 |
Azure
Cielo 6 AIQ060 |
|
Dyes/Probes |
||
SYBR™ Green, EvaGreen™, FAM™ |
x |
x |
VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560 |
x |
x |
TAMRA™ |
|
x |
ROX™, TEX®615, CAL Fluor® Red 610 |
|
x |
Cy®5, Quasar®670, Liz®, Mustang Purple® |
x |
x |
Cy®5.5 |
|
x |
Other
dyes/probes are possible depending on the system configuration. |
MyGo Mini S
- Small format 16 x 0.1 mL QPCR System with Taqman Multiplexing at an amazing price.
- Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and analyze the data at the same time. Windows, Linux and Mac OSX
- Solid state reliability with up to 5-year warranty
Precision and Performance
The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.
For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Easy Analysis
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
Specifications
Number of Reactions |
16 |
Format |
0.1 mL tubes |
Reaction Volume |
10-100 µl |
Temperature Control Method |
Peltier |
Speed |
3°C/s heating | 1.5°C/s cooling |
Temperature Uniformity |
0.05°C (SD) |
Temperature Accuracy |
± 0.25°C |
Temperature Resolution |
0.01°C |
Connection Options |
LAN - up to 400 instruments |
Direct connection - RJ45 |
|
Stand alone - USB stick |
|
Detection |
500 to 650 nm (CMOS array) |
Multiplex Level |
Duplex |
Sensitivity |
Single copy detection |
Dynamic Range |
9-log |
Precision |
1.1 fold discrimination |
Factory Calibrated Dyes |
ATTO 490LS, CAL 540, |
CAL 560, FAM, HEX, |
|
LC Green, ResoLight, ROX, |
|
SYBR Green, SYTO 9, VIC |
|
Choice of Custom Dyes |
Yes |
Supported Assay Formats |
TaqMan |
Intercalators |
|
HRM |
|
Operating Noise |
Silent |
Power |
90W |
Warranty |
Up to 5 years |
MyGo Pro
- Small format 32 x 0.1 mL QPCR System, holds four 8 x 0.1 mL Strips.
- Runs 45 cycles in 33 minutes.
- Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and Analyze the data at the same time. Windows, Linux and Mac OSX.
- Solid state reliability with option of 5-year warranty.
Precision and Performance
The MyGo MPro demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis.
Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.
For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
Easy Analysis
The MyGo Pro system comes with easy-to-use software, and robust, high performance algorithms provide accurate results from complex data. Automated analysis of complex HRM data makes this
powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
Specifications
Dimensions ( w x d x h) | 25 x 27 x 23 cm |
Weight | 7 kg |
Operating noise | <40 db(A) |
Electrical | |
Voltage | 100 - 240V AC ± 10% |
Frequency | 50-60Hz ± 10% |
Power | 170W |
Number of reactions | 32 |
Format | 0.1 mL tubes / 8-tube strips |
Reaction volume | 10 - 100 L |
Temperature control | |
Method | Peltier |
Range | 37-99°C |
Speed | 5°C/s heating, 4°C/s cooling |
Resolution | 0.01°C |
Uniformity | 0.05°C (SD) |
Accuracy | ± 0.25°C |
Run time | < 40 minutes |
Fluorescence | |
Excitation | 500 nm (blue LED) |
Detection | 510 to 750 nm (CMOS array) |
Channels | 120 optical channels |
PCR | |
Sensitivity | Single copy detection |
Dynamic range | 9-log |
Precision | 1.1 fold discrimination |
Factory calibrated dyes | SYBR Green I, ResoLight, FAM, VIC, HEX, Yellow 555, Red 610, TexasRed, Cy5, CAL 540, CAL 560, CAL 590, CAL 610, CAL 635, JOE, Pulsar 650, Quasar 570, Quasar 670, Quasar 705, ROX, TAMRA, TET |
User Chosen Custom Dyes | Yes |
Supported Assay Formats | Intercalating dyes (e.g.) SYBR Green I), Hydrolysis Probes, Molecular Beacons, SimpleProbes, HybProbes |
Connection Options | LAN, Direct connection to computer (RJ45), PC-free (USB stick) |