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PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C₄H₁₀O₂S₂
Solubility: Water
Storage Temperature: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.

• Appearance: A white free flowing powder, hygroscopic
• Solubility: Freely soluble in methanol, methylene chloride
• Clarity in water: 5% (w/v) solution in water is clear and colorless
• Identification by TLC: Rf value of the sample is comparable with that of the standard
• Melting Range: 37.0 - 44.0 °C
• Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
• Assay (By Iodimetry): ≥ 98.0 %
• Country of Origin : Report

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PR1MA™ Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.


Ingredients:

• Total Nitrogen (TN): ~9.8%
• Amino Nitrogen (AN): ~5.1%
• AN/TN Ratio: ~0.52
• Sodium Chloride: ~0.3%
• pH (1% Solution): ~7.0

• Appearance: Light Beige Powder
• Amino Nitrogen: 4.5 - 5.8 %
• Ash Content: 11.50 - 16.0 %
• Dry Matter: ≥ 95.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen: 10.0 - 11.8 %
• Clostridium Perfringens Spores : Report cfu/g
• E.Coli: Report cfu/g
• Molds and Yeasts: Report cfu/g
• Pathenogenic Staphylococcus: Report cfu/g
• Salmonella : Report cfu/25g
• Standard Plate Count: Report cfu/g

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PR1MA™  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C₈H₁₈N₂O₄S
Solubility: Water
Storage Temperature: Room Temperature

Zwitterionic N-substituted aminosulfonic acid buffer.

• pka (25°C): 7.5
• pH Range (Working): 6.8 - 8.2
• Heavy Metals: <0.001% Max

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• DNase, RNase, protease: Not Detected
• Endotoxin (0.2% Solution): ≤ 0.25 EU/ml
• Heavy Metals (as Pb): ≤ 0.001 %
• Identification (IR): Pass
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Solubility (1M Solution in Water ) : Clear and Colorless Solution
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.


Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.


Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride:5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsule (Cream to beige free flowing powder)
• DCM Appearance: Capsule (Homogeneous, free flowing, beige)
• PPM Appearance: Capsule (Clear, no to light ppt, yellow)
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 16.0 g/L
• Yeast Extract: 10.0 g/L
• Sodium Chloride: 5.0 g/l
• Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121^oC for 15 minutes.

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PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 19:1 Ratio

Storage Temperature: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• Solubility: Clear and complete
• Conductivity: ≤ 10 µmhos

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PR1MA™  Water, Molecular Biology Grade, DNase and RNase Free

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H₂O
Storage Temperature: Room Temperature

Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.
 

• Endotoxin Free
• RNase Free
• DNase Free

*Tested to < 0.001 EU/ML


Meets or exceeds CAP/CLSI specifications for Clinical Laboratory Reagent Water (CLRW) and USP/NF Purified Water.


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Clear and Colorless Solution
• Bioburden : No Growth
• DNase: No Visible Degradation
• Protease: No Visible Degradation
• RNase: No Visible Degradation
• Country of Origin: USA

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PR1MA™  Puromycin Dihydrochloride

CAS Number: 58-58-2
Molecular Weight: 544.43
Chemical Formula: C₂₂H₂₉N₇O₅ • 2HCl
Solubility: Water
Storage Temperature: -20°C

Aminonucleoside antibiotic allows selection of cells expressing the pac gene. Inhibits growth of gram positive bacteria and various animal and insect cells.

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to Off White Powder
• Identification (TLC): Conforms
• Melting Point: 168 - 182 ^oC
• Purity (HPLC): ≥ 98.0 %
• Solubility: Clear, colorless or light yellow solution
• Water (KF): ≤ 12.0 %

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PR1MA™  Peptone, Soy
 
CAS Number: 91079-46-8
Solubility: Water
Storage Temperature: Room Temperature

From papaic hydrolysis of soybean meal. Low NaCl content.
 

Research or further manufacturing use only, not for food or drug use.
 

• Amino Nitrogen: ≥ 2.2 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 6.0 %
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 7.0 %
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Salmonella: Negative /25g
• Standard Plate Count : ≤ 5000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Country of Origin of Raw Material : Report
• Raw Material Species: Plant
• Country of Origin of Enzymes : Report
• Enzyme Species: Porcine
• Country Of Manufacture: Report

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PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.


Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

Pall® Ampoule Media for Microbiological Analysis

• Sterile Ampoule Media Pre-Measured for Maximum Efficiency and Convenience
• Meets Membrane Filter (MF) Technique Standard Method requirements with 2 mL ampoules*
• Maximizes efficiency with premixed and presterilized media
• Choose between plastic or glass ampoules for your applications
• Simplify pouring of the media with wide-mouth glass ampoules
• All plastic ampoule media is economically packaged with 50 ampoules per box

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PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

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PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.


pH (1% solution): 7.3.


Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report