Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

 

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

 

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs

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PR1MA™ Agarose SS, Super High Strength, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature

For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules. DNAse/RNase free.


Gel Strength, 1%: >1800 g/cm2
Gel Strength, 1.5%: >3200 g/cm2
Gelling Temperature: 35-37°C
Melting Temperature: 87-89°C
Sulfate: <0.12%
EEO: <0.13%


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Fine white homogeneous powder
• DNase/ RNase: Not Detected
• Gelling Temperature: ≥ 10 ^oC
• Melting Point: 80 - 100 ^oC

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PR1MA™ Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.

Gel Strength, 4%: ≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• EEO: ≤ 0.13 -Mr
• Gelling Temperature: 32.5 - 38.0 ^oC
• Gel Strength : ≥ 1400 g/cm²
• Melting Temperature: ≤ 90.0 ^oC
• Moisture Content: ≤ 10.0 %
• Sulfate: ≤ 0.15 %

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PR1MA™  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C₄H₁₁NO₃ • HCl
Solubility: Water
Storage Temperature: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline
• Assay: 99.0 - 101.0 %
• pH (1% aqueous solution): 4.2 - 5.0
• Solubility (1M Solution in Water ) : Clear, colorless solution
• UV Absorbance @260nm, 1.0 M in H₂O: ≤ 0.06
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.05
• Identification: Conforms
• Heavy Metals : ≤ 5 ppm
• Grade: USP

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PR1MA™  Nystatin

CAS Number: 1400-61-9
Molecular Weight: 926.1
Chemical Formula: C₄₇H₇₅NO₁₇
Solubility: DMSO
Storage Temperature: 2-8°C


Polyene antifungal antibiotic produced by Streptomyces noursei.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Yellow to light tan powder
• Crystallinity: Pass Test
• Identification: Pass Test
• Loss on Drying: ≤ 5.0 %
• pH @25 °C: 6.0 - 8.0
• Potency (Assay): ≥ 4400 U/mg
• Suspendability: Pass Test
• Residual Solvent (n-butanol): ≤ 1000 ppm
• Residual Solvent (Methanol): ≤ 3000 ppm
• Residual Solvent (Acetone): ≤ 5000 ppm
• Residual Solvents: The following Class 1,2, or 3 Solvents may be present in this product. All comply with the limits prescribed in the current USP.

For a Limited Time,
Buy One Order of PR1MA Agarose (500g), Receive Another Order for Free!

Valid through December 31, 2022
This promo cannot be combined with other promotions or discounts. Institutional pricing not valid. All terms apply.

Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling. Manufactured using innovative organic solvent free manufacturing process that is more environment friendly.

Application: Ideal for routine DNA and RNA gel electrophoresis and blotting

Features:

• High purity and Multi-purpose;
  
• Low background and high resolution;
  
• Optimized gel strength for ease of gel preparation

   

  Specifications:

  Appearance	White to off-white powder
  Water	7% max.
  Solubility	Clear colorless solution at 1G plus 100 mL water
  Gel strength(1% Gel)	1200 g/cm2min.
  EEO(Electroendosmosis(-Mr)	0.13 max.
  Sulfate	0.15 max.
  Ash	0.5 max.
  Gelling point(1.5% Gel)	36°C±1.5°C
  Grade	Biotechnology grade
  CAS	NO.9012-36-6

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PR1MA™  MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid]

Common Name: 2-(N-Morpholino)ethanesulfonic acid
CAS Number: 145224-94-8
Molecular Weight: 213.24
Chemical Formula: C₆H₁₃NO₄S • H ₂O
Solubility: Water
Storage Temperature: Room Temperature

Buffer for use in plant tissue culture.

• pKa (20°C): 6.15
• pH range: 5.5 - 6.7

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White crystalline powder
• Assay (Titration): ≥ 99.0 %
• Heavy Metals: ≤ 0.0010 %
• Loss on Drying: 7.0 - 10.0 %
• pH: 2.5 - 4.0
• pKa @20 ^oC: 6.02 - 6.25
• Solubility (10% solution in H₂O): Clear
• Sulfate: ≤ 0.01 %
• UV Absorbance 250nm in 10% H₂O: ≤ 0.05
• Country of Origin : Report

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PR1MA™ bis-Acrylamide, 2% Solution

CAS Number: 110-26-9
Storage Temperature: 2-8°C

A 2% (w/v) bis-Acrylamide solution.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless liquid
• Conductivity of Solution: Report µmhos
• Electrophoresis: Pass Test
• pH: Report

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PR1MA™ Boric Acid

CAS Number: 10043-35-3
Molecular Weight: 61.83
Chemical Formula: H₃BO₃
Solubility: Water
Storage Temperature: Room Temperature

For use in preparing electrophoresis buffers.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Calcium (Ca): ≤ 0.005 %
• Chloride: ≤ 0.001 %
• Heavy Metals (as Pb): ≤ 0.001 %
• Insolubles (Methanol): ≤ 0.005 %
• Iron: ≤ 0.001 %
• Nonovolatiles (Methanol): ≤ 0.05 %
• Phosphate: ≤ 0.001 %
• Purity: ≥ 99.5 %
• Sulfate: ≤ 0.01 %

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PR1MA™  Penicillin G Sodium Salt [Benzyl Penicillin Sodium Salt]

Common Name: Benzyl Penicillin Sodium Salt
CAS Number: 69-57-8
Molecular Weight: 356.4
Chemical Formula: C₁₆H₁₇N₂NaO₄S
Solubility: Water
Storage Temperature: 2-8°C

Inhibitor of bacterial cell wall synthesis. Active against gram positive and some gram negative bacteria. β-Lactamase sensitive.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Potency : 1500 - 1750 U/mg
• Assay: 96.0 - 102.0 %
• Identification: Pass Test
• Loss on Drying: ≤ 1.0 %
• pH: 5.5 - 7.5
• Specific Optical Rotation: +285 - +310 °
• Absorbance 264 nm: 0.80 - 0.88
• Absorbance 280nm: ≤ 0.10
• Absorbance 325nm: ≤ 0.10
• Related Substances: Conforms to standard
• Bacterial Endotoxins : Conforms to standard
• Sterility: Conforms to standard
• Country of Origin : Report

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PR1MA™ Potassium Acetate

CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C₂H₃O₂K
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insolubles: ≤ 0.005 %
• Iron: ≤ 5 ppm
• Magnesium (Mg): ≤ 0.002 %
• pH (5% in water): 6.5 - 9.0
• Phosphate: ≤ 0.001 %
• Purity: ≥ 99.0 %
• Sodium (Na): ≤ 0.03 %
• Sulfate: ≤ 0.002 %
• Country of Origin: Report

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PR1MA™  Blasticidin S Hydrochloride 10 mg/mL Solution

CAS Number: 3513-03-9
Molecular Weight: 458.9
Chemical Formula: C₁₇H₂₆N₈O₅ • HCl
Solubility: Water
Storage Temperature: -20°C

 
A peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates.


Supplied as a 10mg/ml sterile solution in HEPES buffer.
Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Clear Solution
• Concentration: 10mg/ml sterile solution
• Description: Colorless solution in HEPES buffer
• pH: 7.2 - 7.5
• Purity: ≥ 98.0 %