Molecular Biology Reagents/Chemicals

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PR1MA™ LB Broth 1 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. For propagation and maintenance of E.Coli. Low Salt Formula. Molecular Biology Grade.


Add 20 capsules per liter of water. Heat and stir until capsule is completely dissolved. Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.15% solution @25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™  D-Cycloserine

CAS Number: 68-41-7
Molecular Weight: 102.1
Chemical Formula: C₃H₆N₂O₂
Solubility: Water
Storage Temperature: 2-8°C

Interferes with bacterial cell wall synthesis by competing with D-Alanine for incorporation into the cell wall. Active against most mycobacteria with some activity against gram negative bacteria.


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to pale yellowish crystalline powder
• Identification: A blue color gradually develops
• Specific Rotation: +108 - +114 °
• Polymer (285nm): ≤ 0.80
• Crystallinity: Should Comply
• pH: 5.5 - 6.5
• Loss on Drying: ≤ 1.0 %
• Residue on Ignition: ≤ 0.50 %
• Methanol: ≤ 0.30 %
• Ethyl Acetate: ≤ 0.50 %
• Ethanol: ≤ 0.5 %
• Acetone: ≤ 0.50 %
• Acetic Acid: ≤ 0.50 %
• Assay: ≥ 90.0 %
• Grade: USP
• Country of Origin : Report

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PR1MA™ Chloramphenicol

CAS Number: 56-75-7
Molecular Weight: 323.1
Chemical Formula: C₁₁H₁₂Cl₂N₂O ₅
Solubility: Ethanol, Methanol and Slightly Soluble in Water
Storage Temperature: Room Temperature

Bacteriostatic antibiotic with activity against gram positive and gram negative bacteria.


Plant cell culture tested.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White, greyish-white or yellowish-white, fine, crystalline powder or fine crystals, needles or elongated plates
• Identification: Pass
• Melting Point : 149 - 153 ^oC
• pH: 4.5 - 7.5
• Specific Optical Rotation: +17.0 - +20.0 ^o
• Chloride: ≤ 0.01 %
• Related Substances: ≤ 0.50 %
• Loss on Drying: ≤ 0.50 %
• Sulfated Ash: ≤ 0.10 %
• Assay (Dried Basis): 98.0 - 102.0 %
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin : Report

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PR1MA™ NZCYM Broth

Solubility: Water
Storage Temperature: Room Temperature

Enriched medium for cultivating recombinant strains of E.Coli and propagating theta bacteriophage.


Dissolve 22 grams in 1 liter distilled water. Sterilize by autoclaving for 15 minutes.


Casein Digest Protein: 10 g/L
Yeast Extract: 5 g/L
Casamino Acids: 1 g/L
Magnesium Sulfate: 0.98 g/L
Sodium Chloride: 5 g/L


Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2.2 % Solution): 6.8 - 7.2
• Escherichia coli ATCC 23724: Good to Excellent
• Escherichia coli ATCC 33694: Good to Excellent
• Escherichia coli ATCC 33849: Good to Excellent
• Escherichia coli ATCC 39403: Good to Excellent
• Escherichia coli ATCC 47014: Good to Excellent
• Escherichia coli ATCC 53868: Good to Excellent
• Country of Origin : Report

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PR1MA™ Nutrient Broth

Solubility: Water
Storage Temperature: Room Temperature

For cultivating a variety of non-fastidious organisms.


Dissolve 8 grams in one liter distilled water. Heat solution to boiling with continuous stirring for one minute. Sterilize by autoclaving at 121°C for 15 minutes. Store at room temperature.

Beef Extract: 3 g/L
Pancreatic Digest of Gelatin: 5 g/L


Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous Free Flowing,Beige Powder
• pH (0.8 % Solution @25 ^oC): 6.6 - 7.0
• Microorganism (Bacillus Subtlis ATCC 9372): Fair to Good
• Microorganism (Escherichia Coli ATCC 25922): Good to Excellent
• Microorganism (Salmonella Typhimurium ATCC 14028 ): Fair to Excellent
• Microorganism (Staphylcoccus Aureus ATCC 25923): Good to Excellent
• Pancreatic digest of gelatin: 5.0 g/L
• Beef Extract: 3.0 g/L

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PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder, 50:1 Ratio

Storage Temperature: Room Temperature
 

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Powder
• Solubility: Clear Solution
• Conductivity: ≤ 10 µmhos
• Electrophoresis: Pass

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PR1MA™  Bicine Ultra-Pure

CAS Number: 150-25-4
Molecular Weight: 163.2
Chemical Formula: C₆H₁₃NO₄
Solubility: Water
Storage Temperature: Room Temperature

Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.


pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C


Research or further manufacturing use only, not for food or drug use.
  

• Appearance: White Powder
• FTIR: Conforms to reference
• Optical ABS @ 260 NM: ≤ 0.1
• Optical ABS @ 280 NM: ≤ 0.1

PR1MA™ Ethanol, Reagent Grade

Components:

• Ethyl Alcohol      90%
• 2-Propanol          5%
• Methanol            5%

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PR1MA™ Ampicillin, Sodium Salt

CAS Number: 69-52-3
Molecular Weight: 371.4
Chemical Formula: C₁₆H₁₈N₃O₄SNa
Solubility: Water
Storage Temperature: 2-8°C
 
 

Inhibitor of bacterial cell wall synthesis resulting in lysis or deformation of the bacterium. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. B-Lactamase sensitive. Plant cell culture tested. Widely used as a selective agent for transformation of plasmid encoding for b-Lactamase production such as pBR322 or pUC (Ampr).
 
 

• Appearance: White Crystalline Powder
• Assay (Anhydrous Substance) : 91.0 - 102 %
• Bacterial Endotoxins : ≤ 0.15 IU/mg
• Dimethylaniline: ≤ 0.002 %
• Identification: Conform
• Methylene Chloride : ≤ 0.2 %
• pH (1% Solution): 8.0 - 10.0
• Solubility (50mg/ml in Water): Soluble
• Specific Optical Rotation: +258 - +287 °
• Sterility: Conform
• Water: ≤ 2.0 %

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PR1MA™ Agar, Phyto

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.


Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pale Yellow Powder
• Gelling Point: 34 - 36 °C
• Gel Strength : 950 - 1050 g/cm²
• Identification: Conforms
• Loss on Drying: ≤ 18 %
• Melting Point: 85 - 95 ^oC
• Nephelos: ≤ 40 NTU
• pH: 6.0 - 7.5
• Total Ash: ≤ 3.5 %
• Gelatin: Absent
• Starch: Absent
• Total Plate Count: ≤ 1000 cfu/g

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PR1MA™  Phosphate Buffered Saline, 10X Solution

Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing. Ready to use sterile 10X solution.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Solution
• Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
• Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
• pH (1X Solution): 7.2 - 7.6
• pH (10X Solution): 6.8 - 7.2

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PR1MA D-(+)-Biotin [Vitamin H]

Common Name: Vitamin H

CAS Number: 58-85-5

Molecular Weight: 244.3

Chemical Formula: C₁₀H₁₆N₂O₃S

Solubility: Water

Storage Temperature: 2-8°C


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White or off white powder
• Assay: 97.5 - 102.0 %
• Related Substance (HPLC-Individual Impurity): ≤ 1.0 %
• Related Substance (HPLC-Total Impurities): ≤ 2.0 %
• Residual Solvents: To meet requirments
• Specific Rotation: +89 - +93 °
• BSE/TSE Free: BSE/TSE Free
• Grade: USP