Molecular Biology Reagents/Chemicals

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Acrylamide/bis-Acrylamide, 19:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38% (w/v) Acrylamide and 2% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 19:1.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless liquid
• Conductivity of Solution: ≤ 10 µmhos
• DNase/ RNase: Not Detected
• Electrophoresis: Pass Test
• pH: Report

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Luria Broth 1 Gram Buffered Capsules [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1/1185-53-1
Solubility: Water
Storage Temperature: Room Temperature

High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.


RPI Media is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.


Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Growth
• Microorganism (E. coli ATCC 33694): Growth
• Microorganism (E. coli ATCC 33849): Growth
• Microorganism (E. coli ATCC 39403): Growth
• Microorganism (E. coli ATCC 47014): Growth
• Microorganism (E. coli ATCC 53868): Growth

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Homogeneous, Free Flowing Powder
• Color: Light Tan Yellow
• Amino Nitrogen: ≥ 4.0 %
• Moisture : ≤ 6.0 %
• pH: 6.0 - 7.5
• Protein: Report %
• Solution (2%): Clear Light Yellow
• Total Nitrogen: > 7.5 %
• Coliforms: < 10 cfu/g
• Standard Plate Count : < 5000 cfu/g
• Thermophile Count: Report cfu/g
• Source: Bovine

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Lactobacillus MRS Broth Powder

Solubility: Water
Storage Temperature: Room Temperature


Recommended for the selective isolation and enumeration of lactobacilli.

Ingredients:

• Peptic Digest of Animal Tissue: 10.0g
• Beef Extract: 10.0g
• Yeast Extract: 5.0g
• Ammonium Citrate: 2.0g
• Dextrose: 20.0g
• Polysorbate 80:1.0g
• Sodium acetate: 5.0g
• Magnesium Sulfate: 0.1g
• Manganese Sulfate: 0.05g
• Sodium Phosphate, Dibasic: 2.0g

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing with soft lumps, yellow beige to yellow tan
• PPM Appearance: Clear, no to light ppt, medium to dark amber
• Microorganism (Lactobacillus Casei ATCC 393 ): Good to Excellent
• Microorganism (Lactobacillus Fermentum ATCC 9338 ): Good to Excellent
• Microorganism (Lactobacillus Plantarum ATCC 8014 ): Good to Excellent
• Microorganism (Lactobacillus Rhamnosus ATCC 9595 ): Good to Excellent
• pH (5.5 % Solution @25 ^oC): 6.3 - 6.7

Kappa is now PR1MA™ - Same great product, new name
 Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.
 

PR1MA™  G-418 Disulfate

CAS Number: 108321-42-2
Molecular Weight: 692.7
Chemical Formula: C₂₀H₄₀N₄O₁₀ • 2H₂SO₄
Solubility: Water
Storage Temperature: 2-8°C
 

Aminoglycoside antibiotic used as a selective agent in transformation experiments. Alternative for Kanamycin in monocots eg: rice, lollum and graminea which are highly resistant to the latter. Optical rotation +121.2°. Cell culture tested on Bacillus subtilus.

 
Research or further manufacturing use only, not for food or drug use.

 

• Appearance: White Powder
• Absorbance @ 280nm (1mg/mL in H₂O): ≤ 0.015 nm
• Absorbance @ 570nm (100mg/mL in H₂O): ≤ 0.10 nm
• Ammonia: ≤ 1.0 %
• HPLC (Purity) : Report %
• Loss on Drying: ≤ 10.0 %
• pH: 5.0 - 6.0
• Potency (Dry basis): ≥ 700 µg/mg
• Purity (TLC-Spot): ≤ 3 Spots
• Solubility (100mg/ml in H₂O): Colorless Solution
• Specific Rotation: +104 - +121 °
• Non Animal Origin: The product does not come in contact with materials of animal origin.

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™  Potassium Phosphate, Dibasic

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K₂HPO₄
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Aerobic Plate Count:  ≤  1000 CFU/g
• Assay: ≥ 98.0 %
• Chloride (Cl): ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insoluble Matter: ≤ 0.01 %
• Iron: ≤ 0.001 %
• Loss on Drying: ≤ 1.0 %
• Nitrogen Compound: ≤ 0.001 %
• pH (5% Solution): 8.5 - 9.6
• Sodium (Na): ≤ 0.05 %
• Sulfate: ≤ 0.005 %
• Total Coliforms: Negative
• Yeast and Mold Count: ≤ 100 CFU/g

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.


pH (1% solution): 7.3.


Ingredients:

• Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: ~1.0

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• Amino Nitrogen: ≥ 4.0 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 5.0 %
• Nitrites: Negative
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Proteoses: Positive
• Salmonella: Negative/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Raw Material Species: Bovine
• Enzyme Species: Porcine
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : Report

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Sodium Citrate, Trisodium Salt, Dihydrate, [Citric Acid, Trisodium Dihydrate]

Common Name: Citric Acid, Trisodium Dihydrate
CAS Number: 6132-04-3
Molecular Weight: 294.1
Chemical Formula: C₆H₅Na₃O₇  2H₂O
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.0
• Alkalinity: Pass Test
• Chloride: ≤ 50 ppm
• Heavy Metals : ≤ 10 ppm
• Identification Tests: Passes Tests
• Loss on Drying: 10.0 - 13.0 %
• Sulfate : ≤ 150 ppm
• Tertrate: Meets USP Test
• Grade: Meets current USP Specifications

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temperature: Room Temperature

Mixtures of peptides and free amino acids from peptic digest of animal tissue.

• Amino Nitrogen (AN): 2.5-3.5%
• Loss on Drying: <6.0%

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Granular Powder
• Amino Nitrogen Content: ≥ 3.4 %
• Appearance of Solution: Clear and yellow-beige
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10.0 %

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ CHAPS [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate]

Common Name: 3-[(Cholamidopropyl) dimethylammonio]-1-propanesulfonate
CAS Number: 75621-03-3
Molecular Weight: 614.9
Chemical Formula: C₃₂H₅₈N₂SO₇
Solubility: Water
Storage Temperature: Room Temperature


Zwitterionic, non-denaturing detergent for solubilizing membrane proteins.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Identification: Pass Test
• Moisture : ≤ 2.0 %
• Purity: ≥ 99 %
• RNase/DNase/Protease: None Detected
• Solubility (20%, water): Complete / Colorless
• TLC (silica gel: methanol/NH₄OH = 95/5): One Spot

Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

PR1MA™ Chlortetracycline Hydrochloride

CAS Number: 64-72-2
Molecular Weight: 515.3
Chemical Formula: C₂₂H₂₃ClN₂O₈ • HCl
Solubility: Water
Storage Temperature: -20°C

 
Bacteriostatic antibiotic active against gram positive and gram negative bacteria.

 
Protect from light.

 
Research or further manufacturing use only, not for food or drug use.

 

• Appearance: Yellow Crystalline Powder
• Crystallinity: Pass
• Identification: Pass
• Loss on Drying: ≤ 2.0 %
• pH (1% Solution in Water)@ 25°C: 2.3 - 3.3
• Potency: ≥ 900 µg/mg
• Specific Rotation: -250 - -235°

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™  Ticarcillin Disodium Salt

CAS Number: 4697-14-7
Molecular Weight: 428.39
Chemical Formula: C₁₅H₁₄N₂Na₂O ₆S₂
Solubility: Water
Storage Temperature: 2-8°C
 
 

Inhibits bacterial cell wall synthesis. Highly active against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor. Hygroscopic.


Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: White to yellow crystalline powder
• Assay (Ticarcillin Content, dried basis): ≥ 80.0 %
• Assay (Ticarcillin, dried basis): ≥ 800 µg/mg
• Heavy Metals : ≤ 10 ppm
• Identification: Pass Test
• pH: 6.0 - 8.0
• Related Substances (Largest Impurity): ≤ 4.0 %
• Related Substances (Second Major Impurity) : ≤ 2.5 %
• Residual Solvents: Meets the requirements
• Specific Rotation: +172 - +187 °
• Sterility: Meet the requirements
• Water: ≤ 6.0 %
• Bacterial Endotoxins : ≤ 0.05 EU/mg
• Grade: Conforms to USP Specifications