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PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Dissolve 32 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 12 g/L

• DCM Appearance: Homogeneous Free Flowing ,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/l
• Yeast Extract: 5 g/L
• Agar: 12 g/l
• Casein Peptone: 10 g/L

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PR1MA™ LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light beige color, average particle size between 1 to 2mm
• DCM Appearance: Homogeneous, free flowing light beige
• PPM Appearance: Clear, no to light ppt, light yellow
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Malt Extract Agar

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Malt Extract Agar is used for the isolation, cultivation and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.


Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Maltose: 12.75 g/L
• Dextrin: 2.75 g/L
• Glycerol: 2.35 g/L
• Peptone: 0.78 g/L
• Agar: 15.0 g/L
• Final pH (25°C): 4.6 ± 0.2

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
• PPM Appearance: Trace to moderate haze, off white to pale yellow
• pH(3.36% Solution @ 25 ^oC: 4.5 - 4.9
• Microorganism (Aspergillus niger ATCC 16404): Growth
• Microorganism (Candida albicans ATCC 10231): Growth
• Microorganism (Penicillium Roquefortii ATCC 10110): Growth
• Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
• Microorganisms (Microsporum Audouinii ATCC 42558): Growth
• Microorganisms (Microsporum Canis ATCC 36299): Growth
• Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

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PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temperature: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.


Final Solution contains:

• Casein Enzymic Hydrolysate: 20 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 0.5 g/L
• Magnesium Sulfate: 2.4 g/L
• Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH @25 °C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Tryptone, Granulated

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature

Pancreatic digest of casein. Material is specially granulated to reduce dust and increase solubility rate.


Ingredients:

• Total Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: 1.0
• pH (1% solution): ~7.3%

• Appearance: Light Beige
• Amino Nitrogen Content: ≥ 3.5 %
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10 %
• Enzyme Species: Porcine
• Raw Material Species: Bovine

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PR1MA™ LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.


Dissolve 35 grams in 1 Liter distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes


Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 15 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• pH: 6.8 - 7.2
• Solubility: Clear Solution

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PR1MA™ Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature

Purified high gel strength agar with excellent properties for tissue culture and microbiological work.


Molecular Biology Grade


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Cream Powder
• Ash: ≤ 4.5 %
• Gel Point (1.5% Solution): 33 - 38 °C
• Gel Strength (1.5% Gel): ≥ 800 g/cm²
• Loss on Drying: ≤ 12.0 %
• Melting Point (1.5 %): 88.0 - 96.0
• pH (1.5% solution): 6.0 - 8.0

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PR1MA™ Agar Noble

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Ultra-high purity Agar recommended for use in applications where maximum clarity and purity are essential. Thorough bleaching and washing procedures are utilized to remove trace amounts of ash and mineral contaminants.


Concentrations of 3-8 g/L obtains a semi-solid gel.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to pale yellow crystalline powder
• ASH (Residue Ignition): ≤ 1.6 %
• Gelling Point: 32 - 39 °C
• Melting Point: 80 - 95 ^oC
• Moisture : ≤ 12.0 %
• pH (1.5% solution): 5.5 - 7.2

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PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli.


Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10g/l
• Sodium Chloride: 10g/l
• Yeast Extract: 5g/l
• Agar: 12g/l

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Yeast Extract, Powder

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Due to its low NaCl content this quality is well suited for tissue culture.


Ingredients:

• Total Nitrogen (TN): ~9.8%
• Amino Nitrogen (AN): ~5.1%
• AN/TN Ratio: ~0.52
• Sodium Chloride: ~0.3%
• pH (1% Solution): ~7.0

• Appearance: Light Beige Powder
• Amino Nitrogen: 4.5 - 5.8 %
• Ash Content: 11.50 - 16.0 %
• Dry Matter: ≥ 95.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen: 10.0 - 11.8 %
• Clostridium Perfringens Spores : Report cfu/g
• E.Coli: Report cfu/g
• Molds and Yeasts: Report cfu/g
• Pathenogenic Staphylococcus: Report cfu/g
• Salmonella : Report cfu/25g
• Standard Plate Count: Report cfu/g

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.


2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.


Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.


Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride:5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsule (Cream to beige free flowing powder)
• DCM Appearance: Capsule (Homogeneous, free flowing, beige)
• PPM Appearance: Capsule (Clear, no to light ppt, yellow)
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 16.0 g/L
• Yeast Extract: 10.0 g/L
• Sodium Chloride: 5.0 g/l
• Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121^oC for 15 minutes.