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EU Fast Cycler PCR Plate,
PR1MA High Fidelity Hot Start Master Mix
- Leaves an A-overhang for TA cloning
- Ideal for difficult, high GC content sequences
- 10x fidelity of native Taq
- Ideal for long PCR, up to 10kb targets
PR1MA High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, an antibody mediated hot start mechanism, and a proof-reading component for trouble-free PCR reaction assembly and performance.
The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.
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