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Pall® Acrodisc® 13 mm Syringe Filters

Item #: ASACRODISC13 
NEST Cell Culture Inserts

NEST Cell Culture Inserts

  • Innovative hanging design facilitates pipetting.
  • Supplied in multiple well plates: 6-well, 12-well, 24well.
  • PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
  • Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
  • Transparent PET membrane is easy to observe.
  • The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
  • Compatible with most solvents used for fixing and dyeing.


Attention

  • Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
  • During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
  • Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.


Guidelines

  • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
  • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
  • Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
  • Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
  • Check the volume of the culture medium regularly and add fresh medium as needed.
  • The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
  • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
  • Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.


Material

Multiple well plate or dish is made from PS.


Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
SKU Number Filter Media Filter Size Outlet Type Pore size Qty Price Quantity Add to Cart
4427 Nylon 13 mm male slip luer 0.2 µm 100/pkg, 300/cs
817.56
Each
4540 Nylon 13 mm male slip luer 0.2 µm 1000/pkg
1543.32
Each
4426 Nylon 13 mm male slip luer 0.45 µm 100/pkg, 300/cs
817.56
Each
4541 Nylon 13 mm male slip luer 0.45 µm 1000/pkg
1543.32
Each
4550 Nylon 13 mm minispike 0.2 µm 100/pkg, 300/cs
817.56
Each
4561 Nylon 13 mm minispike 0.2 µm 1000/pkg
1445.04
Each
4551 Nylon 13 mm minispike 0.45 µm 100/pkg, 300/cs
817.56
Each
4546 Nylon 13 mm minispike 0.45 µm 1000/pkg
1543.32
Each
4455 PVDF 13 mm male slip luer 0.2 µm 100/pkg, 300/cs
792.72
Each
4457 PVDF 13 mm male slip luer 0.45 µm 100/pkg, 300/cs
794.88
Each
4450 PVDF 13 mm minispike 0.2 µm 100/pkg, 300/cs
817.56
Each
4544 PVDF 13 mm minispike 0.2 µm 1000/pkg
1543.32
Each
4452 PVDF 13 mm minispike 0.45 µm 100/pkg, 300/cs
817.56
Each
4545 PVDF 13 mm minispike 0.45 µm 1000/pkg
1543.32
Each
PL-4423 PTFE 13 mm male slip luer 0.2 µm 100/pkg, 300/cs
817.56
Each
4542 PTFE 13 mm male slip luer 0.2 µm 1000/pkg
1543.32
Each
4422 PTFE 13 mm male slip luer 0.45 µm 100/pkg, 300/cs
817.56
Each
4543 PTFE 13 mm male slip luer 0.45 µm 1000/pkg
1543.32
Each
4552 PTFE 13 mm minispike 0.2 µm 100/pkg, 300/cs
839.16
Each
4553 PTFE 13 mm minispike 0.45 µm 100/pkg, 300/cs
839.16
Each
4555 PTFE 13 mm minispike 0.45 µm 1000/pkg
1543.32
Each
2400 wwPTFE 13 mm minispike 0.2 µm 100/pkg, 300/cs
854.28
Each
2401-25 wwPTFE 13 mm minispike 0.2 µm 1000/pkg
1597.32
Each
2402 wwPTFE 13 mm minispike 0.45 µm 100/pkg, 300/cs
854.28
Each
2403 wwPTFE 13 mm minispike 0.45 µm 1000/pkg
1597.32
Each
Acrodisc Syringe Filters
Pall® Acrodisc® 13 mm Syringe Filters
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