TPP Tissue Culture Tubes

Bullseye Pre-stained Protein Ladder, 10-180 kDa

• Ready to use - no boiling necessary
• Stable at ambient temperature
• 2 reference bands - red at ~75kDa and green at ~25kDa
• Use 5-15µl, depending on the size of the wells
• No freeze-thaw involved
• Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.

Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

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