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Suspension Cell Culture Dishes

Item #: ASNTDISHES 
IBI MIDI Fast-Ion Plasmid

IBIs MIDI and MAXI plasmid kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lystate has been bound to the column. he contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

Sample size: MIDI50 mL for high copy plasmid, 100 mL for low copy plasmid
MAXI- 100 mL for high copy plasmid, 250 mL for low copy plasmid
Format Ion-Exchange Resin Column
Operation Gravity Flow
Binding Capacity MIDI:  500 ug/ MAXI-1mg
Expectant Yield MIDI-up to 200 ug of plasmid DNA/MAAX-up to 1 mg of plasmid DNA
Purity Equal to that obtained by 2X CsCL-Gradient Centrifugation
Operation Time 120 minutes or less
Applications Transfection,: Sequencing, In vitro Trascrioption

PNRNADNA

Source Cell
Bacteria
Isolation type
DNA
SKU Number Size Description Sterile Qty Price Quantity Add to Cart
229623 100 mm x 20 mm Non-Treated with Ring Sterile 300 Dishes/Case
211.00
Each
229638 35 mm x 10 mm Non-Treated with Ring Sterile 960 Dishes/Case
215.00
Each
229653 150 mm x 20 mm Non-Treated with Ring Sterile 100 Dishes/Case
218.00
Each
229656 150 mm x 15 mm Non-Treated with Ring Sterile 100 Dishes/Case
176.00
Each
229663 60 mm x 15 mm Non-Treated with Ring Sterile 500 Dishes/Case
149.00
Each
229692 100 mm x 15 mm Non-Treated with Ring Sterile 300 Dishes/Case
108.00
Each
229693 100 mm x 15 mm Non-Treated with Ring Sterile 500 Dishes/Case
167.00
Each
Cell Culture Dishes
Suspension Cell Culture Dishes
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