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Polypropylene Plasteur Pasteur Pipets

Item #: ASPPPLASTPIP 

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

PNRNADNA

Source Cell
Gel
Isolation type
DNA
SKU Number Size Reservoir Qty Price Quantity Add to Cart
229271 5.75 in. Length No 200 - Individually Wrapped Sterile
111.00
Each
229274 5.75 in. Length No 1000 - Bulk Non-Sterile
141.00
Each
229276 5.75 in. Length No 500 - Bulk Sterile
133.00
Each
229277 5.75 in. Length No 500 - Bulk Non-Sterile
101.00
Each
229281 9 in. Length No 200 - Individually Wrapped Sterile
116.00
Each
229284 9 in. Length No 1000 - Bulk Non-Sterile
150.00
Each
229286 9 in. Length No 500 - Bulk Sterile
135.00
Each
229287 9 in. Length No 500 - Bulk Non-Sterile
102.00
Each
Polypropylene Plasteur Pi
Polypropylene Plasteur Pasteur Pipets
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