For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® STEM
 
ready-to-use, xeno-free (animal origin-free) hydrogel system for hPSCs 3D static suspension culture and scale-up

• Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
• High-quality 3D stem cells for differentiation and organoid formation
• Easy scale-up (from laboratory scale to industrial scale)
• Efficient cell harvesting or subculturing

VitroGel® STEM is a xeno-free (animal origin-free) hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.

This hydrogel system is ready to use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making cell harvesting simple and effective. The 3D stem cell spheroids that are developed using this system can be used for further sub-cultures, patterned differentiations, organoid development, or re-establishing 2D culture morphologies.

“Just add cells” 5 min protocol. No matrix coating required.

VitroGel® STEM is ready-to-use. Just mix with your hPSCs. There is no laborious matrix coating required to maintain and expand your stem cells.

Specifications

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Cell Recovery Solution (100 mL)
 
Enzyme-free cell harvesting solution to recover cells from the hydrogel in 20 min.

VitroGel® Cell Recovery Solution is an enzyme-free, ready-to-use solution to harvest 2D, 3D, and organoids cultured cells efficiently and safely. The solution can recover cells from VitroGel® hydrogels in 20 minutes.

VitroGel® Cell Recovery Solution is room temperature stable, has a neutral pH, and works at 37°C operating temperature. The solution can maintain high cell viability during the recovery process. Harvested cells can be sub-culture in both 2D and 3D cultures.

The VitroGel® Cell Recovery Solution can be used before or after the fixation and stained preparation of hydrogel specimens to ensure high-quality downstream data analysis.

Specifications 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® IKVAV High Concentration
 
Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity 
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® IKVAV High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (IKVAV). IKVAV is the bioactive sequence located on the C-terminal end of the long arm of the α-1 chain of laminin, which is actively involved in different biological activities such as neuronal progenitor cell differentiation, promoting cell adhesion, neurite outgrowth, angiogenesis, and tumor growth.  VitroGel® IKVAV High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® YIGSR High Concentration
 
Laminin-derived functional peptide (YIGSR) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® YIGSR High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration. VitroGel® YIGSR High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® COL High Concentration
 
collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)

• Dilution Solution TYPE 1 contains sucrose for best osmolarity
• Dilution Solution TYPE 2 is a sucrose-free formulation for cells sensitive to the sugar level in the medium

VitroGel® COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo, which shows great potential for tissue engineering and regeneration medicine application. VitroGel® COL High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel® makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Specifications 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Dilution Solution (50 mL)
 
For hydrogel concentration adjustment with the high concentration hydrogels.

• Type 1 - Contains sucrose to maintain the best osmolarity.
• Type 2 - Sucrose-free formulation for cells sensitive to the sugar level in the medium.

VitroGel® Dilution Solution is a ready-to-use solution to be used with the VitroGel® High Concentration hydrogels to adjust the hydrogel concentration for different hydrogel strength.

The solution is room temperature stable with a neutral pH, which maintains a good physiological condition for hydrogel formation and can accelerate the hydrogel formation in combination with the cell medium. The Dilution Solution can be used to prepare a thin gel coating plate or injectable hydrogel with the high concentrations hydrogels.

Specifications

• Ready-to-use to adjust the concentration of VitroGel® High Concentration hydrogels
• Neutral pH
• Room temperature stable
• Transparent
• Compatible with VitroGel® system for 2D & 3D cell culture and hydrogel injection
• Ships and store at room temperature
• Size: 50 mL

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® HEK293
 
Ready-to-use, xeno-free (animal origin-free) hydrogel system for 3D culture of HEK293 cells

VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells.

• Simple 5-minute protocol
• High-quality 3D spheroids
• Easily scale up with flasks and bioreactors
• Easy cell harvesting protocol (Enzyme-free, No trypsin)

VitroGel® HEK293 is a unique system to easily support the rapid 3D growth of HEK293 cells.  Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The high-quality 3D spheroids enhance the cell performance for protein expression and downstream applications. HEK293 cells cultured in VitroGel® HEK293 can easily be scale-up for large-scale bioreactors or spin flasks. By using the VitroGel® Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.

Specifications 

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroGel® Hydrogel Matrix
 
ready-to-use, xeno-free (animal origin-free) functional hydrogel system

• Xeno-free, biological functional hydrogel
• Ready-to-use at room temperature (20 min protocol)
• Supports a wide range of cell types and applications
• Easy, efficient, and enzyme-free cell harvesting in 20 min

VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel® Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications.“Just Add Cells” – The hydrogel matrix is ready to mix with cell suspension directly.

There is no additional adjustment needed. VitroGel® Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.

3D cell culture process in 20 min – “Just add cells”

VitroGel® Hydrogel Matrix is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Specifications

NEST High Efficiency Erlenmeyer Flasks

• The body is made of polycarbonate (PC), which meets the USP Class 6 Standard, with high transparency, has strong impact resistance, oxidation resistance and can withstand a high temperature up to 121°C. Theres a scale made by injection molding on the flask · body to facilitate observing the filled liquid volume.
• The wide-mouth neck design is convenient for sampling and liquid filling.
• No DNA or RNA enzymes, no pyrogens
• Sterilized by E-Beam
• Packaged in sterile bags

Newly added 3 L wide-mouth high-efficiency Erlenmeyer flask

The bottom diameter of the 3-L wide-mouth high-efficiency Erlenmeyer flask is consistent with that of the 3 L high-efficiency Erlenmeyer flask, and its mouth diameter adopts the neck size of the 5 L high-efficiency Erlenmeyer flask, which not only saves space usage, but also increase oxygen flux, so as to increase the efficiency of cell culture.

Material

PC Bottle & HDPE Cap & Nylon Handle, Vent caps with 0.2um hydrophobic filters for gas exchange without contamination.

Application

Ideal for shaker culture applications.

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.