NEST Transfer Cap for High Efficiency Erlenmeyer Flasks

Two-Way Liquid Transfer Cap
The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.

Multifunctional Liquid Transfer Cap
Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.

Inverted Liquid Transfer Cap
The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.

Application

It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.

Product Features

• Closed transfer reduces the risks of contamination during liquid transfer
• The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues
• A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported
• High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance
• Sterility level (SAL) is maintained at 10-6
• Endotoxin-free, and no ingredients of animal origin

ig BL21 (DE3)
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.

Specifications
Competent cell type:                Chemically competent
Derivative of:                            BL21(DE3)
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         1.0 x 109 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
BL21(DE3) Chemically Competent Cells:             50 µl
DNA (or pUC19 Control, 10 pg/µl):                         1 µl
Recovery Medium:                                                 1 ml

Contents & Storage
BL21(DE3) Competent Cells:                -80 °C
pUC19 Control DNA:                            -20 °C
Recovery Medium:                                  4 °C

Genomic Features
BL21(DE3) chemically competent cells have the following features:

• Widely used host background
• T7 Expression Strain
• Deficient in both lon (1) and ompT proteases
• Resistant to phage T1 (fhuA2)
• B Strain

Genotype
F-ompT hsdS(rB- mB-) gal dcm (DE3)

General Guidelines
Follow these guidelines when using BL21(DE3) chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Transformation Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl)
• DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on pre-warmed selection plates.
• Incubate the plates overnight at 37 °C. 

ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.

Number of Colonies per Transformation Test*

• Up to 2x higher efficiency than any competitor
• Exact same Genotype as NEB's 5-Alpha Cells

Specifications
Competent cell type:        Chemically competent
Derivative of:                    5-alpha
Species:                            E. coli
Format:                             Tubes
Transformation efficiency:      1 x 109 cfu/µg pUC19 DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 5-alpha chemically competent cells:  50 µl
DNA (or pUC19 Control, 10 pg/µl):           1 µl
Recovery medium:                                   1 ml

Contents & Storage
ig 5-alpha competent cells:   -80 °C
pUC19 control DNA:                 -20 °C
Recovery medium:                       4 °C

Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.

ig 10B
Intact Genomics ig 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.

• Most efficient chemically competent cells in the industry (up to 10X more efficient!)
• May easily be used in place of NEB 10-Beta, TOP10 from Invitrogen or other common brands
• Significantly more affordable

Specifications
Competent cell type:                Chemically competent
Derivative of:                            DH10B
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         â¥1.0 x 1010 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 10B chemically competent cells:     50 µl
DNA (or pUC19 Control, 10 pg/µl):          1 µl
Recovery medium:                                  1 ml

Contents & Storage

1. ig 10B competent cells: -80 °C
2. pUC19 control DNA: -20 °C
3. Recovery medium: 4 °C

Genomic Features
ig 10B (DH10B derivative) chemically competent cells have the following features:

• 80lacZM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
• mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine

Genotype
F â mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara, leu)7697 galU galK rpsL (StrR) nupG -

General Guidelines
Follow these guidelines when using ig 10B chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 µl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 µl to 200 µl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.

VitroINK® Bioinks

Ready-to-use bioinks for 3D bioprinting

VitroINK® are ready-to-use, xeno-free (animal origin-free) bioink system either unmodified or modified with either RGD, Collagen-memetic, IKVAV, or YIGSR peptides or with Matrix Metalloproteinases (MMP) for biodegradable bioink.

VitroINK® is a family of the ready-to-use, xeno-free tunable bioink system that requires no UV,  no temperature/pH curing, or chemical cross-linking. The system is ready-to-use at room temperature, neutral in pH, transparent, and has excellent visibility after printing and cell culture. Due to the unique shear-thinning and rapid recovery mechanical property, VitroINK® can maintain the printed structure without UV or other special curing methods. Adding cell culture medium after printing can further stabilize the printed structure and support cell growth. Cells can be pre-mix with VitroINK® using our VitroINK® Mixing Kit for mixing ratios at 3:1 or 10:1. Different versions of VitroINK® may incorporate multiple biological functional ligands to promote cell attachment, cell-matrix interactions, cell proliferation, motility/migration and differentiation for many different applications.

Specifications

• Xeno-free tunable modified bioink.
• Ready-to-use at room temperature
• No UV, temperature/pH curing, or chemical cross-linking required
• Neutral pH
• Transparent. Excellent visibility after printing and cell culture
• Pre-mix with cells using the included VitroINK® Mixing Kit
• Ships room temperature. Store at 2-8°C
• Size: 3 mL

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 10:1 Mixing Component Pack
 
Single-use components for 10:1 mixing ratio for VitroINK® Mixing Kit. DISPENSER NOT INCLUDED

The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.

This package is sterilized and disposable for one-time use.

Kit Contents and Specifications

• Disposable mixing kit components for VitroINK® kit:
• (1) 1 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 10:1 mixing ratio
• Ships room temperature. Store at room temperature.

ReadyStrain™ II Sterile Cell Straining Kits

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 3:1 Mixing Component Pack

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® Mixing Kit – Complete Pack
 
Mix VitroINK® bioink with cells at 3:1 and 10:1 ratios. Full mixing kit with Dispenser and single-use components.

Mixing cells with the bioink is a critical step for 3D bioprinting. Because of the differences in viscosity of the cell suspension and bioink, the traditional manual mixing methods create a lot of air bubbles and non-uniform mixture which leads to the unstable printed structure, difficulty for cell observation and affect cell viability.

The VitroINK® Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK® and cell suspension are placed in the mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

The syringe, connector and mixing head are disposable for one-time use. Order the sterilized replacement mixing kit components to ensure the robust results for every single mixing.

Kit Contents and Specifications

• Disposable mixing kit components for VitroINK bioink:
• (1) Dispenser
• (1) 1 mL syringe
• (1) 3 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 3:1 or 10:1 mixing ratio
• Ships room temperature. Store at room temperature.

• New! Compatible with 6 well plates
• Superior ventilation - prevents clogging/spills
• Advanced design - faster flow through
• Interlocking and stackable
• RNase, DNase, pyrogen and cytotoxin free
• Sterile (gamma radiation) and individually packed
• Designed for use with 50 ml tubes (adaptor for 15 ml tubes included)

The Harvest Premium Cell Strainer system is a fast easy-to-use solution for detaching, isolating, and straining cells from clusters and tissues. A polypropylene frame supports a nylon mesh that comes in three sizes: 40 µm (blue), 70 µm (white), and 100 µm (yellow). A tiered filtration process is made possible by the unique interlocking stackable design.
 
Redesigned with  support tabs (for multi-well plates) and 1.5mm feet (for flat dishes and plates). Strainers are supplied sterile, individually wrapped, in convenient blister packs, with one free reducing adapter. 

The advanced frame/housing design offers a unique geometry ensure aseptic handling and eliminate the contamination risks associated with fingers contacting the filtered suspension, mesh and/or interior surfaces. The enhanced performance and improved aeration, by eliminating the "air-lock" effect, also reduces processing time and prevents messy spillovers.

ReadyStrain™ is the first product of its kind, providing all the necessary parts for filtration of cell suspensions and combining them into a single, preassembled, ready to use, sterile packet. The kits eliminate the contamination risks associated with opening multiple packages, and assembling separate pieces. The individual sterile packets mean researchers are no longer forced to expose entire bags of 50mL tubes and corresponding caps to perform one filtration. Instead they can open a single ReadyStrain packet directly under the hood.
  

• Kit Includes: strainer, 50 mL tube & cap
• Opens directly under the hood reducing risk of contamination
• Pre-assembled - ready to use
• Guaranteed sterile - gamma
• Packaged in easy-open "tear-strip" bags