• Higher sensitivity
• Use in the same way as EtBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Safest DNA stain by far
• Low cost
• 10,000X
• Excitation wavelengths at 290nm and 490nm

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.

DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

Samples stained with DNA SafeStain Plus are compatible with downstream molecular biology applications; such as, gel extraction, and cloning.

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290nm & 490 nm
• Emission at 530nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

For the proper disposal of this product, follow University or Company Guidelines.

• The CleanSpot is designed to fulfill a very important laboratory need...control of PCR contamination.
• Just 25 - 30 minutes of UV irradiation in the CleanSpot can prevent unwanted PCR contamination.
• The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.
• The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.
• The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permits safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.
• The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.
• Dimensions: 18"L x 24"W x 28"H
• 1 year warranty

The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.

Includes: Rotocycler 72 and 500 assorted color coding inserts

Accessories for the Rotocycler 72