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2x Master Mix Kit (1.5 mM MgCl2) 

Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.

Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)

Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.

Composition of 2x Taq Master Mix

150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2% Tween 20Ò

4 mM dNTPs

2 units/µL AS ONE Taq polymerase

Inert Red Dye & Stabilizer (BE180303 only)

Item#:
ASPCRREAG3
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R-Taq DNA Polymerase 5 units/µL

Cat. No.

Units

10X Ammonium Buffer (MgCl2 15mM)

MgCl2

25 mM

BE200303

500

1.5 mL

1.5 mL

BE200304

1,000

2x 1.5 mL

2x 1.5 mL

BE200306

2,500

4x 1.5 mL

4x 1.5 mL

Store at -20°C. For in-vitro laboratory use only

 

General Description

Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.

Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.

  • High performance thermostable DNA polymerase
  • Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
  • Leaves an A-overhang

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.

Component

Vol./reaction

Final Conc.

10X Ammonium Buffer

5 µL

1X

dNTP mix (12.5 mM each)

0.8 µL

0.2 mM each dNTP

Primer A

Variable

0.1-0.5 µM

Primer B

Variable

0.1-0.5 µM

R-Taq DNA Pol

1 mL

5 units/reaction

Distilled Water

Variable

- - - -

Template DNA

Variable

0.1-0.5 µg/reaction

TOTAL volume

50 µL

- - - -

Table 2. MgCl2 concentration

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions.

For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

6. Place the tubes in the thermal cycler and start the reaction.

7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.


Tween 20 is a registered trademark of ICI Americas, Inc.


Final MgCl2 conc.

in reaction (mM)

1.5

2.0

2.5

3.0

3.5

4.0

4.5

Additional volume

of 25 mM MgCl2

per reaction (µL):

0

1

2

3

4

5

6

10X Ammonium Reaction Buffer

Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.

Suggested Protocol using R-Taq Polymerase

This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix & template DNA)

Item#:
ASPCRREAG4
Strips, 1000/cs for

These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.

Item#:
T319-4N
Your Price:
465.98
Each
Thick Adhesive, Leaves No Residue Behind


Click to shop new and improved eXTReme™ 2 Seal Extra Strong Sealing Film


eXTReme™ Seal Sealing Film with Extra Strong Adhesive Minimizing Evaporation Without Residue

eXTReme™ Seal features an extra-strong thick adhesive to minimize evaporation, yet removes easily without leaving residue on the plate.

Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.

  • Certified DNase- & RNase- free
  • Ideal for use with raised-rim and non-skirted PCR plates
  • Available in a larger cut for flat-top semi-skirted and full-skirted PCR plates: eXTReme™ Seal, Large

Application

  • PCR
  • Robotics
Temperature Range
  • -20°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended


Item#:
ASPCRSEALFILM
 
eXTReme Seal Features
Specs eXTReme Seal
Sealing Film Selection Guide by Application
Sealing Film Selection Guide by Attributes
Optically Transparent, Highly Conformable Adhesive

For a limited time, purchase any TS-RT2 or TSS-RTQ (100qty) and get a 20% discount!
Offer valid July 1st to October 1st 2022

This promo cannot be combined with other promotions or discounts.  All terms apply. 

ThermalSeal® RT2™ Optically Transparent, Highly Conformable Adhesive, Polyester Sealing Film

Designed for use with standard 96-well plates where rows must be selectively protected or accessed. Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® RT2RR 

  • Certified DNase- & RNase- free
  • Ultra-high optical clarity
  • Minimizes evaporation and cross-contamination
  • Ideal for use with flat-top and hard-shell dual component (e.g. polycarbonate / polypropylene) PCR plates
  • Not recommended for removal after thermal cycling

Application

  • qPCR
Temperature Range
  • -40°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended

Item#:
ASQPCRFILM
 
ThermalSeal RT2 Features
Specs ThermalSeal RT2
Sealing film selection guide by application
Sealing film selection guide by attributes
System w/ Recirculation

Model A5 Buffer Puffer 
Large Gel Electrophoresis System with Built-in Recirculation
Gel Size: 20cmW x 25cmL
Footprint: 28cmW x 38cmL x 15cmH
Running Buffer Volume: 2.0L
Ideal for High Resolution Genomic Applications

The Owl Buffer Puffer, Model A5 recirculation system offers convenience and versatility. The Buffer Puffer recirculation system prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Buffer Puffer is ideal for long runs, multiple sample sets or RNA gels. The Buffer Puffer delivers clear results for samples run over long time periods. It also eliminates uneven migration, band distortion or disassociation of pH dependent glyoxylated RNA molecules that can result when ionic depletion occurs. Because the recirculation system is built right into the buffer chamber, no external pumps, tubing or stir bars are required.

Complete System Includes:

  • Buffer Chamber with Three Point Leveling Base
  • SuperSafe Lid with Attached Power Supply Leads
  • U.V. Transmissible (UVT) Gel Tray with Gasketed End Gates
  • 3 Combs: 16, 24, and 36 Well 1.5mm Thick
  • 3 year warranty
Item#:
A5
Your Price:
1180.00
Each
This item is discontinued
On Target Promo!

On Target Promo!
*Receive a free VM1-B-F (BV101-B), MF12-F (C1012) or E5001-SD-F (E5001-SD)
 *Choose 1
 

-- Up to $400 Value --
With the purchase of E3100

See details HERE 
 Valid through October 31st, 2022. Institutional pricing not valid. All terms apply  


myView™ Compact UV Transilluminator

  • Uniform Illumination | Four 302 nm Bulbs
  • Adjustable UV Safety Cover | Easy Access to Gels
  • Large Scratch Resistant Viewing | 16.5 x 13.5 cm

The E3100 Compact UV Transilluminator has a surprisingly small footprint (just over 12 x 8 inches) yet provides industry leading performance. It will accommodate one or two mini gels on the 16.5 x 13.5 cm scratch resistant viewing surface.

The integrated UV blocking cover is made from a polymer that will not fluoresce in UV light. This allows clear viewing of DNA bands through the cover yet offers full UV blocking protection. Two friction hinges on the front edge of the cover allow for full adjustment. Position the cover horizontally for general viewing, tilt it at an angle for gel access and band cutting, or open fully to use the SmartDoc™ Imaging Enclosure (sold separately) for gel imaging with a smartphone.

The transilluminator’s four 6W bulbs evenly illuminate the viewing surface, and the 302 nm wavelength is ideal for Ethidium Bromide, SmartGlow™, SYBR Green®, Gel Green™ and many other common gel stains.  Spacing of the UV bulbs, coupled with a reflector system and special diffusing filter glass provides exceptional uniformity across the viewing surface. This allows for quantification of DNA and higher quality imaging when used with a camera system.



Specifications

Light source 4 x 6W UV Light Bulbs

4 x 6W UV Light Bulbs

Safety cover UV blocking polycarbonate

UV blocking polycarbonate

Exterior dimensions

10 x 8 x 2.75 in
25.4 x 20.32 x 6.99 cm

Electrical

12VDC (AC power adapter included)

Output wavelength

Peak at 302 nm

Viewing surface

6.5 x 5.3 in. | 16.5 x 13.5 cm

Weight

0.9 kg | 2 lbs

Warranty

2 years

Item#:
ASUVBENCHTRANS
System w/ Recirculation

The Owl Buffer Puffer Horizontal Recirculating System combines all of the convenience and versatility of the EasyCast system with a unique buffer recirculation design that prevents the formation of pH and ionic gradients. The Buffer Puffer System is ideal for long runs, multiple sample sets or RNA gels. The Buffer Puffer System delivers clear results for samples run over long time periods. It eliminates uneven migration, band distortions or disassociation of pH dependent glyoxylated RNA molecules that can result when ionic depletion occurs. Because the recirculation system is built right into the buffer chamber, no external pumps, tubing or stir bars are required.

Comb Options
Catalog
Number		 Comb
Type		 Number
of Teeth		 Thickness
of Teeth		 Width
of Teeth		 Recommended Loading Volumes
0.25cm 0.5cm 0.75cm 1.0cm
B2-12 Double Sided 12 1.0
1.5mm		 7.2
7.2mm		 5
8µl		 18
28µl		 32
49µl		 46
69µl
B2-16 Double Sided 16 1.0
1.5mm		 5.4
5.4mm		 4
6µl		 14
21µl		 24
36µl		 34
52µl
B2-20 Double Sided 20 1.0
1.5mm		 3.9
3.9mm		 3
4µl		 10
15µl		 18
26µl		 25
37µl
B2-24 Double Sided 24 1.0
1.5mm		 3
3.0mm		 4
5µl		 13
19µl		 21
32µl		 30
45µl
B2-8C Standard 8 1.0mm 12.5mm 9µl 33µl 56µl 80µl
B2-8D Standard 8 1.5mm 12.5mm 14µl 49µl 84µl 120µl
B2-PREP Prep 2 1.5mm 106/5mm 120/5.5µl 415/20µl 710/34µl 1000/48µl
B2-RL-25C Rapid Load 25(2X) 1.0mm 2.5mm 2 µl 7µl 12µl 17µl
B2-RL-25D Rapid Load 25(2X) 1.5mm 2.5mm 3µl 11µl 18µl 26µl
B2-RL-9C Rapid Load 9(1X) 1.0mm 7.2mm 5µl 19µl 32µl 46µl
B2-RL-9D Rapid Load 9(1X) 1.5mm 7.2mm 8µl 28µl 49µl 69µl
B2-WALL Wall 1 1.5mm 120mm  

 

Complete System Includes:

  • Buffer Chamber
  • SuperSafe Lid with attached power supply leads
  • EasyCast gasketed U.V. Transmissable (UVT) Gel Tray
  • 2 Combs: 12 and 20 well, double-sided, 1.5/1.0mm thick
  • 3 year warranty
Item#:
B3
Your Price:
840.00
Each
White Light, 21x26cm, Single

Uniform white light diffusion for viewing negative and film positives. X-ray film, autoradiographs, microtiter plates, and stained gels can be viewed on the white light transmitting glass surface. This surface, permanently double-safety sealed into the unit for durability, provides a scratch and chemical resistant work area which can be cleaned easily. Durable transilluminator housing is scratch resistant, epoxy powder paint finish for easy cleaning. 2 year warranty

ModelOrder NoDescriptionFilter SizeDimensions
TW-26 95-0208-01White Light Transilluminator, 115V 21 x 26cm9.5"D x 13.25"W x 4.25"H
TW-4395-0214-01White Light Transilluminator, 115V 36 x 43cm19.13"D x 16"W x 4.25"H

Safety Certifications: ETL, ETL/c on all 115V models.
Item#:
95-0208-01
Your Price:
767.14
Each
On Target Promo!

On Target Promo!
Receive a free E4000-VG1 Viewing Glasses Order ($48 Value)
With the purchase of SB4500
See details HERE 
 Valid through October 31st, 2022. Institutional pricing not valid. All terms apply  

The SmartBlue™ Blue Light Transilluminator 

 
The SmartBlue™ Blue Light Transilluminator is the smart alternative to UV transillumination. The uniform 465 nm bottom lighting illumination is perfect for excitation of most green fluorophores without damaging the DNA, thus resulting in a much higher downstream cloning efficiency. Furthermore, the two position filter cover position allows for easy access to the gel for band excision. Without the need for the use of DNA damaging UV light, you don't have to worry about any damage to the skin and eyes.


The LEDS are long lasting (with a 30,000 hour service life) providing a durable and efficient illumination option. Gels can be excised directly on the unit with the scratch resistant surface. The unit contains an automatic 5 minute timed shut off for energy saving as well. The cover can be placed flush with the unit, at a 60 degree angle, or completely removed for use with the SmartDoc Imager (E5000-SD).

Pair with DNA Safe Stain (C138) for the ultimate viewing effectiveness!

A 230V is available upon request (SB4500-E).

Specifications

Light Source

High Intensity LEDs

Filter Cover

Amber, for filtering of blue light

Exterior Dimensions

12.8 x 8.5 x 1.9 in | 30.5 x 21.5 x 5 cm

Electrical

100-240 V, 50/60 Hz

Output Wavelength

Peak at 465 nm

Viewing Surface

6.6 x 4.75 in | 17 x 12 cm

Weight

1.2 kg | 2.6 lb

Warranty

2 years



SmartBlue™ Mini Blue Light Transilluminator

Safer for samples, safer for you: Accuris SmartBlue™ Mini Blue Light Transilluminator is the perfect partner for most common safe DNA stains. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  The SmartBlue™ Mini also works for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images. Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*


Two position filter cover:  The E4100 transilluminator comes with an emission filter cover. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers are designed to fit flat for general gel viewing or to be placed at an angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely. UI technology, bright and clear images:  The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Mini Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.


Reliable, durable and long lasting:  An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Mini Transilluminator. Unlike those in a UV transilluminator, the filters in the SmartBlue™ Min system will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Mini Transilluminators are covered by a 2 year warranty.


* The SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 
 
 
Specifications 

Light Source

High Intensity LEDs

Filter Cover

Amber, for filtering of blue light

Exterior Dimensions

6.7 x 4.7 x 1.9 in | 17 x 12 x 5 cm

Electrical

100-240 V, 50/60 Hz

Output Wavelength

Peak at 465 nm

Viewing Surface

3.9 x 4.1 in | 10 x 10.5 cm

Weight

0.2 kg | 0.5 lb

Warranty

2 years


Optional SmartBlue™ Plus Viewing Glasses


Accuris SmartBlue™ Plus Viewing Glasses offer exceptional protection for researchers working with bright blue LED and UV light illuminators. The polycarbonate filter lenses are shatter proof, scratch resistant and block light in the UV (280 nm) to blue (490 nm) wavelengths. In addition, they are compliant with ANSI Z87.1.

 

These glasses are ideal for general protection when working with hazardous wavelengths and for precision DNA band cutting on a UV or blue light transilluminator. While blocking hazardous UV and blue light, the glasses allow clear visualization of DNA bands stained with Ethidium Bromide, SmartGlow™, SYBR Green and other fluorescent dyes. 
 
*Always wear appropriate UV protection for eyes and exposed skin when working with UV light sources 

 

Item#:
ASSMARTBLUELGT

Accuris qMAX One-Step RT-qPCR kits 
  • RNA to cDNA to qPCR, in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for preparation at room-temperature
  • Blue dye facilitates pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation available for multiple target amplification
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Bulk Packaging: 
To accommodate the requirements of high throughput users, Accuris is now offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs. 

 
Item#:
ASACSYBRGRONE
 
PR2120 qMax Green-One-Step RT qPCR Product Insert
PR2121 One-Step Probe qPCR Product Insert
  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • 2 year warranty

Visi-Blue Transilluminators are designed for use with GFP stains. As compared to UV, the blue light will transmit through almost any transparent (plastic, glass, etc.) laboratory equipment. This is essential when monitoring and detecting DNA migration and protein expression in real time.

  • Two models are available with surface areas as follows: 21x26cm or 20x40cm
  • Select from models with 8-watts or 25-watts
  • Amber cover (included) blocks blue light transmission and allows visualization of media above 500nm
Item#:
ASPCRUVEQ10
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