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Buy 5 Cases of TPP Cryo Tubes
and Get a PR1MA™ Cooler Cryo Rack Free! (#Rack-Cryo-F)
Valid through December 31, 2022 - Institutional pricing not valid. All terms apply.
TPP Polar Cryogenic Tubes
Perfect for long term storage of Cell lines or Harvested Tissues in the freezer or cryogenic dewars (LN2 vapor phase)
- Pronounced external thread for gas and aerosol tight seal
- Enables a fitted seal to prevent leaking during storage, transportation or centrifugation
- Protects sample integrity by reducing contamination
- The external threading results in a smooth internal surface that eliminates undue loss of specimen material during removal.
- Uniquely designed vial geometry voids the use of silicone gaskets
- Self-standing tube with star bottom permits one handed opening/closing
- Manufactured with the highest quality of virgin raw materials
- Minimizes sample interaction with the centrifuge tube material and prevents leaching that decreases sample integrity
- No additives, slip agents, biocides or plasticizers are utilized in the manufacturing process
- White writing surface for easy labeling
- On side of tube permits detailed description labeling
- Ideal pairing with labeling TPP cap inserts
- Highly visible white & accurate graduations
- Supplied in convenient, puncture and tear resistant re-closable bags with zipper and laser perforation
- Permits ergonomic open/close under sterile conditions
- Application based options available
- Cell line freezing & long term storage
- Sample storage and secure transport
- Long term storage of harvested tissues
Quality Assurance:
- Sterile (gamma irradiated)
- DNAse/RNAse, pyrogen, and heavy metal-free
Recommendations:
- Always use appropriate safety equipment and practices when handling frozen polar tubes
- TPP Polar Tubes are to be used for storage in the gas/vapor phase
- Avoid filling up the Polar Tubes to the maximal filling volume to avoid spillage of liquid sample due to expansion of liquids upon freezing
Material |
Temperature Range |
Max. RCF |
Polypropylene (PP) Tubes |
-196°C to 121°C |
9,500 x g |
- Available in 6-, 12-, 24-, 48-, and 96-well format
- Polystyrene, NOT Tissue Culture Treated (suspension culture)
- Sterile (gamma irradiated)
Need an order expedited? Contact your sales rep or email Tech@midsci.com
Looking for mats? Click here! Looking for sealing film? Click here!
Deep Well Plates and Combs for KingFisher Systems
FEATURING:
Ultra-flat surfaces
Alphanumeric grid reference
Raised Well Design - improved heat sealing and reduced cross contamination
Storage down to -80°C
Automation compliant footprint (SBS/ ANSI/SLAS specification)
Made from Virgin Polypropylene; no mold release agents or regrind
RNase, DNase, DNA, and Pyrogen Free Certification
MID-KF05US-9-N
Our 250 ul 96-well elution plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems. The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads. Wells also have a conical V-shaped bottom to help concentrate precipitates.
Maximum Volume: 350 ul
Working Volume: 250 ul
Volume with Mat: 250 ul
Matching silicone cap mats: MID-MATS-9-PRT, MID-MATS-9-BULK, MID-SQ-9-PRT
MID-KF10US-24-N
Our 5.0 mL 24-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification (of larger sample sizes, 5 mL) performed on automated separation systems. The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads. Wells also have a conical V-shaped bottom to help concentrate precipitates.
Maximum Volume: 10.0 mL
Working Volume: 5.0 mL
Volume with Mat: 5.0 mL
Matching silicone cap mats: MID-MATS-24-N
MID-KF22US-9-N
Our 2.0 mL 96-well plate is ideal for magnetic bead-based nucleic acid and protein affinity purification performed on automated separation systems. The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads. Wells also have a conical V-shaped bottom to help concentrate precipitates.
Maximum Volume: 2.0mL
Working Volume: 1.0mL
Volume with Mat: 1.0mL
Bullseye Pre-stained Protein Ladder, 10-180 kDa
- Ready to use - no boiling necessary
- Stable at ambient temperature
- 2 reference bands - red at ~75kDa and green at ~25kDa
- Use 5-15µl, depending on the size of the wells
- No freeze-thaw involved
- Broad range ladder
Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.
The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.
Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.
Usage:
For each application, 5-15µl is needed, depending on the size of the wells.
Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.
Storage: -20 °C.
Note for image:
Autoradiography Cassette Aluminum 5x7
Classic Push-Button Cassettes
- Constructed From Lightweight Aluminum & Nylon
- Special Curved Door Gives Exceptional Screen-Film Contact
RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!
- RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
- RunBlue gels incorporate a stacking gel that has been dyed (red for SDS) for the simplest ever sample loading. In addition, there is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
- Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus. Â 10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
- The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
- RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.
Benefits of RunBlue Precast Gels
- 2 year shelf life - never throw away another gel
- Up to 10x stronger - no more torn, ruined gels
- Load up to 20µl - see everything in your sample
- Dyed stacking gel - makes gel loading child's play (SDS = Red)
- No comb to remove - no more broken or bent teeth
- Full length resolving gel - improved resolution and better blotting
- 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
- Fast delivery with immediate and expert technical support for total peace of mind
- Best value gels available - save money!
MAXI Flex Tube Kit
MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Supporting tray (for electro elution protocol) 1ea. (select kits)
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
- Tube volume: 3ml
- Dialysis volume: 0.1-3ml
- Min. sample size for extraction: 20µg
- Max. gel slice: 2cm x 1cm
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:
kilo Daltons |
base pairs |
1K |
3 bp |
3.5K |
11bp |
6-8K |
18-24bp |
12-14K |
36-42bp |
25K |
76bp |
50K |
152bp |
APPLICATIONS
- Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification
MIDI Flex Tube Kit
MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Supporting tray (for electro elution protocol) 1ea. (select kits)
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
- Tube volume: 800µl
- Dialysis volume: 50-800µl
- Min. sample size for extraction: 0.5µg
- Max. gel slice: 1cm x 0.5cm
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:
kilo Daltons |
base pairs |
1K |
3 bp |
3.5K |
11bp |
6-8K |
18-24bp |
12-14K |
36-42bp |
25K |
76bp |
50K |
152bp |
APPLICATIONS
- Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification.
MEGA Flex Tube Kit
MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
- Tube volume: 10ml, 15ml or 20ml
- Dialysis volume: 3-20ml
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins
APPLICATIONS
- Dialysis or buffer exchange with volumes between 3-20ml
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification
MINI Flex Tube Kit
MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.
KIT CONTENTS
- Flex Tubes 2/10/30/50/ 100 pieces
- Supporting tray (for electro elution protocol) 1ea. (select kits)
- Floating rack (for dialysis protocol) 1ea. (select kits)
- Information and Protocol Manual 1ea.
SPECIFICATIONS
- Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
- Tube volume: 250µl
- Dialysis volume: 10-250µl
- Min. sample size for extraction: 0.5µg
- Max. gel slice: 0.4cm x 1.1cm
- Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
- Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:
kilo Daltons |
base pairs |
1K |
3 bp |
3.5K |
11bp |
6-8K |
18-24bp |
12-14K |
36-42bp |
25K |
76bp |
50K |
152bp |
APPLICATIONS
- Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis, such as antibodies and recombinant protein purification
- Removal of contaminating micro-molecules
- Tissue culture extraction purification
- Removal of salts, surfactants, solvents, and detergents
- Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
- pH and buffer adjustment of sample solutions, protein extraction or cell extraction
- High throughput dialysis
- Peptide dialysis, as small as 10 amino acids
- Virus-particles purification
- Molded, durable construction
- Rubber gates for easy, tapeless gel casting
- Adjustable height combs
- Loading guides aid in well visualization
Enduro Horizontal Gel Boxes have been designed for ease of use, safety and durability. A variety of sizes are available, from a mini gel system to a large maxi gel system that can accommodate as many as 450 samples in a single run. A wide range of accessories are available to customize your system, including multichannel compatible gel combs.
QUALITY CONSTRUCTION - The Enduro gel tanks are molded for leakproof performance and a long service life. The corrosion resistant platinum electrodes are located in cassettes to protect them during cleaning and provide for easy replacement. The lids fit on the gel boxes in only one direction. A drip ring on the lid recycles condensed buffer and prevents it from dripping directly onto the gel.
EASE OF USE - Casting gels for the Enduro system is very easy. Simply snap the rubber gates onto the ends if the tray, place the comb in position and pour the gel - no taping is necessary. Slots on the sides of the gel trays hold the combs in place. The two piece combs are designed so that the teeth can be raised or lowered to vary the depth of the well within the gel. The supplied combs are 1.5 mm thick. Combs of other thicknesses are available separately. After the gel has hardened, the combs and gates are removed and the gel and tray placed in the gel box. Loading strips may be placed on the bottom of the gel box to aid in well visualization. Gel trays are UV transparent.
DESIGNED FOR SAFETY - The power leads for the Enduro system are attached to the gel box lid. The connection with the electrodes is made only when the lid is properly in place. Removal of the lid removes power from the system. Side tabs and pressure pads make lid removal easy and prevent sloshing of buffer.
Product Specifications | ||
Enduro 7.7 | ||
Unit Dimensions | 9 x 21 x 9cm | |
Gel Size | 7 x 7cm | |
Maximum Samples | 32 | |
Buffer Volume | 225mL | |
Enduro 7.10 | ||
Unit Dimensions | 9 x 21 x 9cm | |
Gel Size | 7 x 10cm | |
Maximum Samples | 64 | |
Buffer Volume | 225mL | |
Enduro 10.10 | ||
Unit Dimensions | 12.5 x 22 x 9cm | |
Gel Size | 10 x 10cm | |
Maximum Samples | 100 | |
Buffer Volume | 300mL | |
Enduro 15.10 | ||
Unit Dimensions | 17.5 x 26.5 x 9cm | |
Gel Size | 15 x 10cm | |
Maximum Samples | 140 | |
Buffer Volume | 500mL | |
Enduro 15.15 | ||
Unit Dimensions | 17.5 x 26.5 x 9cm | |
Gel Size | 15 x 15cm | |
Maximum Samples | 210 | |
Buffer Volume | 500mL | |
Enduro 20.20 | ||
Unit Dimensions | 23 x 39.5 x 9cm | |
Gel Size | 20 x 20cm | |
Maximum Samples | 450 | |
Buffer Volume | 1200mL | |
Construction | Molded Acrylic | |
Safety | Unidirectional safety lid | |
UV Transparency | Gel tank, trays and lid | |
Gel Casting | Rubber gates supplies | |
Combs | Height-adjustable | |
Comb Thicknesses | 0.75, 1, 1.5 and 2mm |
Combs for E1015-10 & E1015-15
The Enduro 96 is an ideal complement to the Labnet Enduro Horizontal Gel Box line. The 96-well format unit is compatible with 8-channel pipettes and matches up with the standard 96 well plate configuration. The average run time is 15-30 minutes. The gel is in a 10 X 12 cm format with options for either 1 or 2 marker lanes. The layout of wells is designed to allow a run length of 1.8 cm per sample providing ideal separation. For those who already own an Enduro E1010, the Enduro 96 comb sets are available and compatible with these existing units.
Features include:
- 96 well format
- MCP compatible
- For high throughput analysis of PCR products
Specifications:
Gel Dimensions |
10 X 12 cm |
Unit dimensions |
22 X 12.5 X 9 cm |
Buffer Volume |
300 mL |
Max sample capacity |
96 samples plus 1 or 2 markers |
Combs/number of samples |
8 + 1X marker; 8 X 2X marker |
Thickness |
1; 1.5 mm |