ION Biosciences PBFI K+ Salt
 
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K+) measurements. Ex/Em: 340/505 nm can be used to measure K+-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K+-free PBFI. It is 1.5X more selective for K+ over Naand works well in cytosolic conditions where [K+]/[Na] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K+ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

PBFI K+ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.

Yellow-green fluorescent, potassium (K+) indicator, membrane
 

impermeable. Lower K+ affinity than IPG-2 and IPG-4.
 

ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).
 

IPG-1 TMA+ salt is a membrane impermeable form of IPG-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.

 

Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Brilliant Sodium Assays
 

ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 

ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).

Yellow-green fluorescent, sodium (Na+) indicator, membrane permeable. Higher Na+ affinity than ING-1.

 

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na+) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na+ indicator for high-throughput screening applications targeting Na+ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.

ION Biosciences Fura-2 LR AM
 

Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane permeable.

 

UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Green fluorescent, calcium (Ca²+) indicator, membrane permeable.

 

Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.

Calcein AM
 

Green fluorescent, live cell indicator.
 

Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.

MDR Activity Kit
 
ION’s Multidrug Resistance (MDR) Activity kit is an effective solution for detecting MDR1 and MRP1 activity and compounds susceptible to MDR–mediated efflux. ION’s MDR Activity kit is compatible with fluorescence microscopy, flow cytometry, and fluorescence plate readers using FITC/fluorescein settings.

Calcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.

When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.

ION Biosciences RATIOMETRIC CALCIUM ESSENTIALS
 

Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
 

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca2+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2.
 

Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.