Green fluorescent, calcium (Ca²+) indicator, membrane permeable.

 

Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
 

Lower K+ affinity than IPG-2 and IPG-4.
 

ION Potassium Green – 1 (IPG-1) is the best in class yellow-green fluorescent, intracellular potassium (K+) indicator for measuring changes at most intracellular K+ concentrations. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Kd = 18 mM) or IPG-4 (Kd = 7 mM).

ION Biosciences Fura-2 K+ Salt
 

Ratiometric calcium (Ca²+) indicator, membrane impermeable.

 

Fura-2 is the most popular UV-excitable, ratiometric green indicator for calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

 

Fura-2 K+ salt is a membrane impermeable form of Fura-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION VITAL – MitoVolt
 

Mitochondrial membrane potential assay kit that uses JC-10, a membrane potential sensitive dye that is more soluble than JC-1.
 

ION Vital – MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. ION Vital – MitoVolt is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.
 

JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence.
JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.
 

ION Vital – MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, ION Vital – MitoVolt provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.

ION Biosciences Fura-2 AM
 

UV-excitable, ratiometric green calcium (Ca²+) indicator, membrane permeable.

 

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Yellow-green fluorescent, sodium (Na+) indicator, membrane impermeable. Higher Na+ affinity than ING-1.

 

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na+) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na+ indicator for high-throughput screening applications targeting Na+ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
 

ING-2 TMA+ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced

Brilliant Calcium Kits

High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.

ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.

Brilliant Calcium Flex Kit

Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.


Contents of Brilliant Calcium Flex Kit
 

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable

 

Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.

 

Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences ICR-1 K+ Salt
 
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
 

ICR-1 K+ salt is a membrane impermeable form of ICR-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ION Biosciences IPG-2 TMA+ Salt
 
Yellow-green fluorescent, potassium (K+) indicator, membrane
 

impermeable. Lower K+ affinity than IPG-4 and higher affinity than IPG-1
 

ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
 

IPG-2 TMA+ salt is a membrane impermeable form of IPG-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods

SBFI AM
 

Ratiometric sodium (Na+) indicator, membrane permeable
 

SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.