Molecular Biology Reagents/Chemicals

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PR1MA™ Distilled Water, Laboratory Reagent Grade

CAS Number: 7732-18-5
Molecular Weight: 18.02
Chemical Formula: H₂O

Laboratory reagent grade. Purified by steam distillation for use as a research laboratory reagent. Not intended or sold for human consumption. 10 Liter size supplied in a Cubitainer with pouring spout.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear Liquid
• Alkalinity (Total as CaCO₃): ≤ 20 ppm
• Aluminum (Al): ≤ 0.2 ppm
• Antimony (Sb): ≤ 0.006 ppm
• Arsenic (As): ≤ 0.01 ppm
• Barium (Ba): ≤ 2 ppm
• Beryllium (Be): ≤ 0.004 ppm
• Boron (B): ≤ 1 ppm
• Bromate: ≤ 0.010 ppm
• Bromide: ≤ 0.005 ppm
• Cadmium (Cd): ≤ 0.005 ppm
• Calcium (Ca): ≤ 2 ppm
• Chloride (ASTM D5442): ≤ 50 ppb
• Chromium (Cr): ≤ 0.10 ppm
• Cobalt (Co): ≤ 0.15 ppm
• Conductivity (ASTM D1125): ≤ 5.0 µS/cm
• Copper (Cu): ≤ 1 ppm
• E.Coli: ≤ 1 MPN/100ml
• Fluoride : ≤ 4 ppm
• Foaming Agents: ≤ 0.5 mg/l
• Hardness (as CaCO3): ≤ 10 mg/l
• Heterotrophic Bacteria (F1094): ≤ 100 cfu/10ml
• Iron (Fe): ≤ 0.3 ppm
• lead (Pb): ≤ 0.015 ppm
• Magnesium (Mg): ≤ 0.10 ppm
• Manganese (Mn): ≤ 0.05 ppm
• Mercury (Hg): ≤ 0.002 ppm
• Molybdenum (Mo): ≤ 0.15 ppm
• Nickel (Ni) : ≤ 0.005 ppm
• Nitrate as N: ≤ 10 ppm
• pH (ASTM D1293): 5.0 - 7.0
• Phosphorous (P): ≤ 1 ppm
• Potassium (K) : ≤ 1 ppm
• Resistivity (ASTM D1125): ≥ 0.2 megaohm
• Scandium (SC): ≤ 0.15 ppm
• Selenium (Se): ≤ 0.05 ppm
• Silica (ASTM D4517) : No Limit ppb
• Silver (Ag): ≤ 0.10 ppm
• Sodium (ASTM 6071): ≤ 50 ppb
• Standard Plate Count: ≤ 500 cfu/ml
• Strontium (Sr): ≤ 0.15 ppm
• sulfate: ≤ 250 ppm
• Thallium (Tl): ≤ 0.002 ppm
• Tin (sn): ≤ 0.15 ppm
• Titanium (Ti): ≤ 0.15 ppm
• TOC (ASTM 5997): No Limit ppm
• Total Coliform: ≤ 1 MPN/100ml
• Total Radium: 0.5835 - 1.491 pCi/l
• Uranium (U): ≤ 0.030 ppm
• Vanadium (V): ≤ 0.15 ppm
• Zinc (Zn) : ≤ 5 ppm

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PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Dissolve 32 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 12 g/L

• DCM Appearance: Homogeneous Free Flowing ,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/l
• Yeast Extract: 5 g/L
• Agar: 12 g/l
• Casein Peptone: 10 g/L

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PR1MA™ PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder

CAS Number: 7778-77-0; 7447-40-7; 7558-79-4; 7647-14-5
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Uniform Powder (No Granular)
• Chloride: 0.136 - 0.144 moles/liter
• Conductivity @ 25 ^oC: 14000 - 17800 µomhos/cm
• DNase, RNase, protease: Not Detected
• pH (10X Solution): 6.7 - 6.9
• pH (1 X Solution): 7.3 - 7.5
• Solubility: Pass Test (9.89%)
• Seal Integrity Testing: Pass Test

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PR1MA™  Sodium Phosphate Dibasic, Anhydrous, ACS Grade

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na₂HPO₄
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystals
• Assay: ≥ 99.0 %
• Chloride: ≤ 0.002 %
• Heavy Metals : ≤ 10 ppm
• Insoluble Matter: ≤ 0.01 %
• Iron: ≤ 0.002 %
• Loss on Drying: ≤ 0.2 %
• pH (5% Solution): 8.7 - 9.3
• Sulfate: ≤ 0.005 %
• Yeast and Mold Count: ≤ 100 CFU/g
• Aerobic Plate Count: ≤ 1000 CFU/g

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PR1MA™ LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light beige color, average particle size between 1 to 2mm
• DCM Appearance: Homogeneous, free flowing light beige
• PPM Appearance: Clear, no to light ppt, light yellow
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

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PR1MA™  Paromomycin Sulfate

CAS Number: 1263-89-4
Molecular Weight: 615.6
Chemical Formula: C₂₃H₄₅N₅O₁₄ • H₂SO₄
Solubility: Water
Storage Temperature: Room Temperature

Aminoglycoside antibiotic with mode of action similar to Kanamycin and Neomycin. Used as a selective agent for the incorporation of the NPT II (APH3') gene in plant tissue. Plant culture tested.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Powder
• Assay: ≥ 675 U/mg
• Identification: Conforms
• Loss on Drying: ≤ 5.0 %
• pH 3.0% Solution @25^oC: 5.0 -7.5
• Specific Optical Rotation: +50.0 - +55.0 °

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PR1MA™ Acrylamide/bis-Acrylamide, Premixed Powder 29:1 Ratio

Storage Temperature: Room Temperature

Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Conductivity of Solution: ≤ 10.0 µmhos
• Electrophoresis: Pass Test
• Solubility: Pass Test

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PR1MA™ Malt Extract Agar

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Malt Extract Agar is used for the isolation, cultivation and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.


Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Maltose: 12.75 g/L
• Dextrin: 2.75 g/L
• Glycerol: 2.35 g/L
• Peptone: 0.78 g/L
• Agar: 15.0 g/L
• Final pH (25°C): 4.6 ± 0.2

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
• PPM Appearance: Trace to moderate haze, off white to pale yellow
• pH(3.36% Solution @ 25 ^oC: 4.5 - 4.9
• Microorganism (Aspergillus niger ATCC 16404): Growth
• Microorganism (Candida albicans ATCC 10231): Growth
• Microorganism (Penicillium Roquefortii ATCC 10110): Growth
• Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
• Microorganisms (Microsporum Audouinii ATCC 42558): Growth
• Microorganisms (Microsporum Canis ATCC 36299): Growth
• Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

• RNase & DNase Free Water A new process of purifying large quantity of DNase and RNase free water enables us to offer Molecular Grade Water at a price that any laboratory can afford. 
  
  
• No toxic agents such as DEPC are used in making the Molecular Grade Water; therefore, there is no chance of DEPC interfering with enzymatic reactions. 
  
  
• The Molecular Grade Water is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase and RNase contamination. 
  
  
• Molecular Grade Water can be used for making reagents and rinsing glass/plastic wares for DNA and RNA research. 
  
  
• Each lot is quality tested for the absence of RNase and DNase contamination. Test reports are attached with each container as an added assurance.

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PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temperature: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.


Final Solution contains:

• Casein Enzymic Hydrolysate: 20 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 0.5 g/L
• Magnesium Sulfate: 2.4 g/L
• Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH @25 °C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™  Nourseothricin Sulfate [NTC] [clonNAT] [Streptothricin Sulfate]

Common Name: [NTC] [clonNAT] [Streptothricin Sulfate]
CAS Number: 96736-11-7
Solubility: Water
Storage Temperature: 2-8°C

Dihydrogen sulfate form of the weekly basic antibiotic Nourseothricin consisting of components F, E and D. Effective against gram negative and gram positive bacteria. Also effective against mycobacteria, mycoplasms, protozoa, yeasts, viruses and plants. Commonly used as a selection agent for recombinant yeast strains.

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White Powder
• Carbon (C): Report %
• Hydrogen (H): Report %
• Nitrogen (N): Report %
• Purity (HPLC): ≥ 85 %
• Sulfur (S): Report %

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PR1MA™  Bacitracin

CAS Number: 1405-87-4
Molecular Weight: 1422.69
Chemical Formula: C₆₆H₁₀₃N₁₇O₁₆ S
Solubility: Water, Ethanol, and Methanol
Storage Temperature: 2-8°C

Interferes with bacterial cell wall synthesis. Active against gram positive bacteria. Toxic to plant cells.


Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: White to pale buff powder
• Identification: Pass test
• pH: 5.5 - 7.5
• Loss on Drying: ≤ 5.0 %
• Residue on Ignition: ≤ 0.5 %
• Bacitracin A: ≥ 40.0 %
• SUM of bacitracin A, B1, B2 and B3: ≥ 70.0 %
• Limit of early eluting peptides: ≤ 20.0 %
• Limit of Bacitracin F: ≤ 6.0 %
• Microbial Assay (Dried Basis): ≥ 65.0 IU/mg
• Heavy Metals : ≤ 30 ppm
• Total Viable Aerobic Count: ≤ 100 CFU/g
• Particle Size (< 20µm by volume): ≥ 99.0 %
• Crystallinity : Pass
• Appearance of Solution: Clear, colorless to slight hazy yellow
• Source: Bacillus Licheniformis
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin : Report