Molecular Biology Reagents/Chemicals

MIDSCI gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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No current replacements available at this time.  Please find other currently available buffers here.


PR1MA  Tricine, Ultra Pure [N-[Tris (Hydroxymethyl) Methyl] Glycine]

Common Name: N-[Tris (Hydroxymethyl) Methyl] Glycine
CAS Number: 5704-04-1
Molecular Weight: 179.17
Chemical Formula: C6H13NO5
Solubility: Water
Storage Temperature: Room Temperature
 

  • pKa @ 25°C: 8.1-8.2
  • Melting Range: 182-188°C

Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis):  99.0 %
  • Heavy Metals :  5 ppm
  • pH (1% Solution): 4.6 - 5.3
  • Solubility (1M Solution in H2O): Clear
  • UV Absorbance 260nm (1 mol/L, 1cm ):  0.04
  • UV Absorbance 280nm (1 mol/L, 1cm ):  0.02
  • Water Content :  0.1 %

PR1MA Formamide, Super Pure

CAS Number: 75-12-7
Molecular Weight: 45.04
Chemical Formula: CH3NO
Storage Temperature: 2-8°C

For nucleic acid hybridization and sequencing

Shipping Weight: 0.1 lbs
Shipping Dimensions: 3.38 x 4.63 x 3.38


Specifications
Appearance: Clorless Liquid
Assay: ≥ 99.5 %
Freezing Point: 1.0 - 3.0 °C
Copper: ≤ 0.0001 %
Iron: ≤ 0.0005 %
Lead (Pb): ≤ 0.0001 %
Zinc: ≤ 0.0001 %

Research or further manufacturing use only, not for food or drug use.

Item#:
ASFORMAMIDE

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PR1MA Sucrose

CAS Number: 57-50-1
Molecular Weight: 342.3
Chemical Formula: C12H22O11
Storage Temperature: Room Temperature

Ultra-high purtiy, double crystallized to remove any remaining inorganic and organic contamination. Produces a clear colorless solution. Used for cultivating protoplasts and "in vitro" multiplication of plant tissue.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Fine White Crystals
  • Absorbance 260nm:  0.07 nm
  • Absorbance 280nm:  0.05
  • DNase, RNase, protease: Not Detected
  • Heavy Metals:  0.0005 %
  • Identification (IR): Pass
  • Purity:  99.0 %
  • Solubility (10% solution in H2O @ 25oC): Pass
  • Sulfate:  0.005 %

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PR1MA Tryptone, Granulated

CAS Number: 91079-40-2
Solubility: Water
Storage Temperature: Room Temperature


Pancreatic digest of casein. Material is specially granulated to reduce dust and increase solubility rate.


Ingredients:

  • Total Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: 1.0
  • pH (1% solution): ~7.3%


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Light Beige
  • Amino Nitrogen Content:  3.5 %
  • Ash:  15.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Total Nitrogen Content:  10 %
  • Enzyme Species: Porcine
  • Raw Material Species: Bovine

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PR1MA  bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane]
 

Common Name: bis(2-Hydroxyethyl)aminotris (hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Weight: 209.2
Chemical Formula: C8H19NO5
Solubility: Water
Storage Temperature: Room Temperature

 
Research or further manufacturing use only, not for food or drug use.
 
  • Appearance: White crystalline powder
  • Assay:  99.0 %
  • Identification (IR): Matches reference
  • Melting Point: Report oC
  • pH, 1.0M Aqueous solution @25ºC: 9.5 - 11.0
  • pKa @ 20°C: 6.0 - 7.0
  • Solubility: Complete and clear
  • UV Absorbance @280nm, 1.0 M in H2O:  0.40

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PR1MA Citric Acid Anhydrous

CAS Number: 77-92-9
Molecular Weight: 192.1
Chemical Formula: C6H8O7
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Fine white crystalline powder
  • Assay (Titration):  99.5 %
  • Carbonizable Substances (H2SO4): Pass Test
  • Clarity and Color: Pass Test
  • Identification: Pass Test
  • Lead :  0.5 mg/kg
  • Limit of Oxalic Acid:  0.036 %
  • Oxalate Test: Pass Test
  • Residual Solvents: Meet requirements
  • Residue on Ignition:  0.05 %
  • Sulfate:  0.015 %
  • Water:  0.5 %
Premixed Powder 37.5:1 Ratio

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PR1MA Acrylamide/bis-Acrylamide, Premixed Powder 37.5:1 Ratio

CAS Number: 79-06-1, 110-26-9
Storage Temperature: Room Temperature


Pre-measured powder blend of acrylamide and bis-acrylamide for simple stock solution preparation.


WARNING!
This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White powder
  • Conductivity of Solution:  10 µmhos
  • Electrophoresis: Pass Test
  • Solubility: Pass Test

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PR1MA LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature


Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.


Dissolve 35 grams in 1 Liter distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes


Ingredients:

  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Agar: 15 g/L



Research or further manufacturing use only, not for food or drug use.


  • Appearance: Powder
  • pH: 6.8 - 7.2
  • Solubility: Clear Solution

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PR1MA Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temperature: Room Temperature


For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivaiton of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.


Research or further manufacturing use only, not for food or drug use.


  • DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
  • PPM Appearance: Pale to light yellow (10X Solution)
  • pH (0.67% Solution @25 oC): 5.2 - 5.6
  • Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
  • Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
  • Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

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PR1MA Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature


Purified high gel strength agar with excellent properties for tissue culture and microbiological work.


Molecular Biology Grade


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to Cream Powder
  • Ash: ≤ 4.5 %
  • Gel Point (1.5% Solution): 33 - 38 °C
  • Gel Strength (1.5% Gel):  800 g/cm2
  • Loss on Drying:  12.0 %
  • Melting Point (1.5 %): 88.0 - 96.0
  • pH (1.5% solution): 6.0 - 8.0

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PR1MA
Lysozyme from Chicken Egg White, Salt Free

CAS Number: 12650-88-3

Solubility: Water

Storage Temperature: 2-8°C
 

Purified from chicken egg white, 2x crystallized, dialyzed and supplied as a salt free lyophilized powder.

Dry powder stable for 3 - 5 years when stored dry at 2°C to 8°C.

Stock solution is stable up to 3 days at 4°C and up to 1 year at -20°C.

Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: 2X Crystallized; dialyzed, lyophilized powder
  • A 280@ mg/ml: Report
  • Protein: Report %
  • U/mg dw (Unit Per mg Dry Weight):  8000
  • Raw Material Species: Chicken Egg Whites
  • Country of Origin: Report

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PR1MA  Colistin Sulfate

CAS Number: 1264-72-8
Molecular Weight: 2801.27
Chemical Formula: (C52H98N16O13 )2  5(H2SO4)
Solubility: Water
Storage Temperature: Short Term: 2-8°C; Long Term: -20°C


Active against gram negative bacteria. Potency approximately 20,000 units per milligram.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White or almost white powder
  • Arsenic (As):  2 ppm
  • Heavy Metals : ≤ 20 ppm
  • Identification: Conforms
  • Loss on Drying:  3.5 %
  • pH (1% in H2O): 4.0 - 6.0
  • Potency (Dried basis):  19000 µg/mg
  • Solubility (50mg/ml in H2O): Clear and light yellow solution
  • Specific Rotation: -73 - -63 °
  • Sulfate: 16.0 - 18.0 %
  • Sulfated Ash:  1.0 %
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