Molecular Biology Reagents/Chemicals

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PR1MA™  Polymixin B Sulfate

CAS Number: 1405-20-5
Molecular Weight: 1385.61
Chemical Formula: C₅₅H₉₆N₁₆O₁₃ • 2H₂SO₄
Solubility: Water
Storage Temperature: 2-8°C

Mixture of sulfates of polypeptides from certain strains of Bacillus polymixa. Active against gram negative bacteria.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Light Yellow Powder
• Identification: Pass Test
• pH (0.5% Solution): 5.0 - 7.5
• Loss on Drying: ≤ 7.0 %
• Single Impurity: ≤ 3.0 %
• Total impurities: ≤ 17.0 %
• Bacteria Colonies: ≤ 10/g
• Ecoli Colonies: Should not be found
• Fungi and Yeast colonies: ≤ 10/g
• Assay, HPLC (on dried basis) (Sum of polymxin B1,B2, B3 and B1-1): ≥ 80.0 %
• Assay, HPLC (on dried basis) (Polymyxin B3): ≤ 6.0 %
• Assay, HPLC (on dried basis) (Polymyxin B1-1): ≤ 15.0 %
• Assay (Dried Basis): ≥ 6000 U/mg
• Assay (Wet Basis): Report U/mg
• Non Animal Origin: Non Animal Origin
• Grade: USP
• Country of Origin: Report

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PR1MA™ Agar Noble

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Ultra-high purity Agar recommended for use in applications where maximum clarity and purity are essential. Thorough bleaching and washing procedures are utilized to remove trace amounts of ash and mineral contaminants.


Concentrations of 3-8 g/L obtains a semi-solid gel.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to pale yellow crystalline powder
• ASH (Residue Ignition): ≤ 1.6 %
• Gelling Point: 32 - 39 °C
• Melting Point: 80 - 95 ^oC
• Moisture : ≤ 12.0 %
• pH (1.5% solution): 5.5 - 7.2

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PR1MA™ Water, HPLC Grade

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H₂O
Storage Temperature: Room Temperature

Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.

• Endotoxin Free
• RNase Free
• DNase Free

*Tested to < 0.001 EU/ML


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Solution
• TOC (ppb): ≤ 3 ppb
• UV Absorbance (1 cm cell Vs Water) @ 190nm: ≤ 0.01
• UV Absorbance (1 cm cell Vs Water) @ 200 nm: ≤ 0.010
• UV Absorbance (1 cm cell Vs Water) @ 210 nm: ≤ 0.010
• UV Absorbance (1 cm cell Vs Water) @ 250 nm: ≤ 0.005
• UV Absorbance (1 cm cell Vs Water) @ 254 nm: ≤ 0.005
• UV Absorbance (1 cm cell Vs Water) @ 280 nm: ≤ 0.005
• Filtered through Ultrafiltration (0.01 Micron): Conforms
• Country of Origin: USA

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PR1MA™ Acrylamide/bis-Acrylamide, 37.5:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38.96% (w/v) Acrylamide and 1.04% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 37.5:1.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, colorless solution
• Conductivity of Solution: ≤ 10 µmhos
• Electrophoresis: Pass Test
• pH: Report

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PR1MA™ Sodium Acetate, Anhydrous

CAS Number: 127-09-3
Molecular Weight: 82.0
Chemical Formula: C₂H₃O₂Na
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 99.0 %
• pH (5% Solution): 7.0 - 9.2
• Solubility: Soluble in Water
• Loss on Drying (120 ^oC): ≤ 1.0 %
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.002 %
• Heavy Metals (as Pb): ≤ 0.001 %
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Magnesium (Mg): ≤ 0.002 %
• Phosphate: ≤ 0.001 %
• Sulfate (SO₄): ≤ 0.003 %
• Grade: Meet USP/ACS Specifications

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PR1MA™  Spectinomycin Dihydrochloride Pentahydrate
 

CAS Number: 22189-32-8
Molecular Weight: 495.3
Chemical Formula: C₁₄H₂₄N₂O₇ • 2HCl • 5H₂O
Solubility: Water
Storage Temperature: 2-8°C

Aminocyclitol antibiotic that inhibits protein synthesis. Cell culture tested.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White or almost white, Slightly hygroscopic powder
• Identification: Pass Test
• pH @25 °C: 3.8 - 5.6
• Potency : ≥ 603 IU/mg
• Residue after Ignition: ≤ 1.0 %
• Solubility: Clear and colorless to light yellow
• Specific Rotation: +15 - +21 °
• Water (KF): 16.0 - 20.0 %

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PR1MA™  Phleomycin 20mg/ml Solution

CAS Number: 11006-33-0
Molecular Weight: 1526.5 g/mol
Chemical Formula: C₅₅H₈₅O₂₁N₂₀S ₂Cu • HCl
Solubility: Water
Storage Temperature: -20°C


Supplied at a concentration of 20 mg/ml.

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Clear Blue Solution
• Concentration: = 20 mg/ml
• Odor: None
• Solubility (20 mg/ml in Water): Freely soluble
• Sterile Filtered: Pass test
• UV Absorbance 244nm (200 ug/ml): 0.75 - 1.05

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PR1MA™ Sodium Chloride

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli.


Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10g/l
• Sodium Chloride: 10g/l
• Yeast Extract: 5g/l
• Agar: 12g/l

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™  Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C₁₅H₁₈N₄O₅
Solubility: Ethanol
Storage Temperature: 2-8°C

 
Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cylce non specific agent, most active in the late G1 and early S phases.


WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Blue violet solid
• Identification (UV): Conforms
• Purity-HPLC (RP CH₃CN:H₂O-0-100%): ≥ 99.0 %
• Purity-HPLC (RP MEOH:H₂O-90-10): ≥ 98.0 %
• Purity-TLC (CH₂CL₂:MEOH- 9:1): ≥ 98.0 %
• Solubility (10 mg/ml MEOH): Clear Blue Solution

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PR1MA Thiamine Hydrochloride [Vitamin B1]

Common Name: Vitamin B1

CAS Number: 67-03-8

Molecular Weight: 337.3

Chemical Formula: C₁₂H₁₇CIN₄OS • HCl

Solubility: Water

Storage Temperature: 2-8°C



Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: White Crystalline Powder
• Appearance of Solution: Clear, no more color than reference solution Y7 or GY7
• Identification: Should be positive reaction
• Identification (IR): Consistent with the reference IR spectra
• pH: 2.7 - 3.3
• Sulfates: ≤ 300 ppm
• Limit of Nitrate: No brown ring is produced at the junction of the two layers
• Lead: ≤ 2 ppm
• Water: ≤ 5.0 %
• Residue on Ignition: ≤ 0.1 %
• Absorbance of Solution: ≤ 0.025
• Residual Solvents (Methanol): ≤ 0.3 %
• Residual Solvents (Ethanol): ≤ 0.5 %
• Related Substance (Impurity A): ≤ 0.15 %
• Related Substance (Impurity B): ≤ 0.3 %
• Related Substance (Impurity C): ≤ 0.15 %
• Related Substance (Any Other Impurity): ≤ 0.1 %
• Related Substance (Total impurities): ≤ 0.5 %
• Related Compounds: ≤ 1.0 %
• Chromatographic Purity: ≤ 1.0 %
• Assay: 98.5 - 101.0 %
• Staphylococcus Aureus: Negative
• Salmonella: Negative
• E.Coli: Negative
• Total Plate Count: ≤ 1000 cfu/g
• Yeast and Mold: ≤ 100 cfu/g
• Material Source: Acrylonitrate; Acetamidine HCl; Chlorine Ester
• Production Method: Chemical Synthesis
• BSE/TSE Free: BSE/TSE Free
• Non Animal Origin: Non Animal Origin
• Conclusion: This product complies with the specification of BP/USP/FCC.
• Country of Origin: Report

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PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C₄H₁₀O₂S₂
Solubility: Water
Storage Temperature: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.

• Appearance: A white free flowing powder, hygroscopic
• Solubility: Freely soluble in methanol, methylene chloride
• Clarity in water: 5% (w/v) solution in water is clear and colorless
• Identification by TLC: Rf value of the sample is comparable with that of the standard
• Melting Range: 37.0 - 44.0 °C
• Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
• Assay (By Iodimetry): ≥ 98.0 %
• Country of Origin : Report