Molecular Biology Reagents/Chemicals

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PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™  Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane]

Common Name: Tris (Hydroxymethyl) Aminomethane
CAS Number: 77-86-1
Molecular Weight: 121.1
Chemical Formula: C₄H₁₁NO₃
Solubility: Water
Storage Temperature: Room Temperature


Highly purified for use in molecular biology applications.

Moisture: <0.2%
Color: <20 APHA (colorless)
Heavy Metals (as Pb): <0.0001%
Insoluble Matter: <0.005%
Heavy Metals: 5 ppm
pKa (20°C): 8.1 ± 0.1


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White Crystalline Powder
• Purity (Dried Basis By GC): ≥ 99.9 %
• Related Substances By GC: ≤ 0.1 %
• Water: ≤ 0.2 %
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 1.0 %
• Melting Point: 170 - 172 ^oC
• APHA Color, 20% Aqueous solution: ≤ 20
• Insoluble Matter: ≤ 0.005 %
• Heavy Metals (as Pb): ≤ 5 ppm
• Residue on Ignition: ≤ 0.5 %
• Iron (Fe): ≤ 5 ppm
• UV Absorbance @260nm, 10% Aqueous solution : ≤ 0.03
• UV Absorbance @280nm, 10% Aqueous solution : ≤ 0.02
• UV Absorbance @290nm, 40% solution in H₂O: ≤ 0.20
• UV Absorbance @430nm, 10% Aqueous solution : ≤ 0.004
• Identification A: Pass
• Identification B: Pass
• Identification C: Pass
• pH (5% in water): 10.0 - 11.5
• Arsenic (As): ≤ 1 ppm
• Chloride: ≤ 100 ppm
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: None Detected
• Lead: ≤ 1 ppm
• Magnesium (Mg): ≤ 1 ppm
• pH (1M in H₂O): 10.5 - 11.5
• Residual Solvents: Conforms to USP/EP
• Residue on Ignition (USP): ≤ 0.1 %
• Solubility (2M In H₂O): Pass

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PR1MA™  PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution]

Common Name: Phosphate Buffered Saline 1X Sterile Solution
CAS Number: 7647-14-5, 7447-40-7, 7558-79-4, 7778-77-0, 7732-18-5
Solubility: Water
Storage Temperature: Room Temperature


Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.

Ready to use sterile 1X solution.


Ingredients:

• Sodium Chloride: 137 mM
• Potassium Chloride: 2.7 mM
• Phosphate Buffer: 11.9 mM
• pH: 7.4
• Does not contain Calcium or Magnesium

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Clear and Colorless Solution
• pH: 7.25 - 7.55

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PR1MA™  Doxycycline Hydrochloride
 
CAS Number: 10592-13-9
Molecular Weight: 480.9
Chemical Formula: C₂₂H₂₄N₂O₈ • HCl
Solubility: Water
Storage Temperature: 2-8°C

 
Tetracycline antibiotic active against gram positive and gram negative bacteria. Interferes with bacterial cell wall synthesis.
 

Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: Yellow crystalline powder
• Absorbance (349nm): 300 - 335
• Ethanol: 4.3 - 6.0 %
• Heavy Metals : ≤ 50 ppm
• Identification (A, B, C): Pass Test
• Light Absorbing Impurities (490nm): ≤ 0.5
• pH: 2.0 - 3.0
• Potency : 800 - 920 µg/mg
• Purity: 95.0 - 102.0 %
• Related Compound (6-Epidoxycyline): ≤ 2.0 %
• Related Substance (Any Other Impurity): ≤ 0.5 %
• Specific Optical Rotation: -120 - -105 ^o
• Sulfated Ash: ≤ 0.4 %
• Water: 1.4 - 2.8 %

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PR1MA™  Metronidazole

CAS Number: 443-48-1
Molecular Weight: 171.2
Chemical Formula: C₆H₉N₃O₃
Solubility: Dilute acids
Storage Temperature: Room Temperature

 
Derivative of 5-Nitroimidazole with activity against anaerobic protozoa and anaerobic bacteria. Protect from light.

 
Soluble in:
Water (10 mg/ml at 20°C)
Ethanol (5 mg/ml)
Methanol
Chloroform (<0.5 mg/ml)
DMSO (34 mg/ml at 25°C)

 
WARNING! This product can expose you to Metronidazole, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

• Appearance: White or yellowish powder
• Assay (Dry Basis): 99.0 - 101.0 %
• Identification: Conform
• Sulfated Ash: ≤ 0.1 %
• Non Animal Origin: Non Animal Origin
• Country of Origin : Report

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PR1MA™ Acrylamide/bis-Acrylamide, 29:1 Ratio Solution

CAS Number: 79-06-1; 110-26-9; 7732-18-5
Storage Temperature: 2-8°C

A 40% solution containing 38.67% (w/v) Acrylamide and 1.33% (w/v) bis-Acrylamide for a monomer to crosslinker ratio of 29:1.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear Colorless Solution
• Conductivity of Solution: ≤ 10 µmhos
• Electrophoresis: Pass Test
• pH @25 ^oC: Report

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PR1MA™  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C₈H₁₇N₂O₄SNa
Solubility: Water
Storage Temperature: Room Temperature

• pKa (20°C): 7.45-7.65
• pH Range: 6.8-8.2

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay (Titration, dried basis): ≥ 98.0 %
• Chloride (Cl): ≤ 0.1 %
• Heavy Metals (as Pb): ≤ 1 ppm
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 2.0 %
• pH (1% aqueous @ 25 ^oC): 9.4 - 11.0
• pKa @ 20°C: 7.45 - 7.65
• Solubility (1M Solution in H₂O): Clear, complete and colorless
• UV Absorbance @ 250nm 1M: ≤ 1.0

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PR1MA™ Ammonium Acetate

CAS Number: 631-61-8
Molecular Weight: 77.08
Chemical Formula: C₂H₇NO₂
Solubility: Water
Storage Temperature: 2-8°C

Widely used reagent in molecular biology and chromatography.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline hygroscopic powder
• Assay: ≥ 97.0 %
• Chloride (Cl): ≤ 0.0005 %
• Heavy Metals (as Pb): ≤ 0.0005 %
• Insoluble Matter: ≤ 0.005 %
• Iron (Fe): ≤ 0.0005 %
• Nitrate: ≤ 0.001 %
• pH, 5% solution in H₂O @25 ºC: 6.7 - 7.3
• Residue after Ignition: ≤ 0.01 %
• Sulfate: ≤ 0.001 %

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PR1MA™ Phosphate Buffered Saline, 10X Solution

Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature

Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 10X solution.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Solution
• Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
• Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
• pH (1X Solution): 7.2 - 7.6
• pH (10X Solution): 6.8 - 7.2

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PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.


Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.


Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Urea, UltraPure (USP Grade)

CAS Number: 57-13-6
Molecular Weight: 60.06
Chemical Formula: CH₄N₂O
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay: 99.0 - 100.5 %
• Identification (A, B): Pass Test
• Appearance of 20% Solution: Pass Test
• Melting Point: 132.5 to 134.5 ^oC
• Moisture (LOD): ≤ 1.0 %
• Alcohol- Insoluble Matter: ≤ 0.04 %
• Residue on Ignition: ≤ 0.1 %
• Chloride: ≤ 5 ppm
• Sulfate: ≤ 0.001 %
• Alkalinity: To pass test
• Ammonium: ≤ 0.05 %
• Urea Related Compound A: ≤ 0.1 %
• Individual Unspecified Impurities: ≤ 0.1 %
• Total impurities: ≤ 2.0 %
• Heavy Metals: ≤ 0.001 %
• Iron: ≤ 0.001 %
• Residual Solvents USP 467: Meets requirements
• BSE/TSE Free: BSE/TSE Free
• Grade: USP
• Country of Origin : Report

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PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

• To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Cream Color, Average Particle Size Between 1-2 mm
• Granulometry (ASTM) 60 Mesh Sieve Size: Report %
• Ash: ≤ 6.5 %
• E.Coli: Negative
• Gel Point (1.5% Gel): 33 - 38°C
• Gel Strength (1.5% Gel): 600 - 1100 g/cm²
• Melting Point: 88 - 96°C
• Moisture : ≤ 15 %
• Molds and Yeasts: < 100 cfu/g
• pH: 6.0 - 8.0
• Total Plate Count: < 1000 cfu/g
• Viscosity (1.5 Solution @ 60 ^oC): ≤ 30 cps
• Water Absorption: ≤ 75 cc