Molecular Biology Reagents/Chemicals

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PR1MA™ Acrylamide

CAS Number: 79-06-1
Molecular Weight: 71.1
Chemical Formula: C₃H₅NO
Solubility: Water
Storage Temperature: Room Temperature

WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Acrylic Acid : ≤ 10 ppm
• Assay: ≥ 99.9 %
• Conductivity: ≤ 3.5 µmhos
• DNase, RNase, protease: Not Detected
• Electrophoresis: Pass Test
• Iron: ≤ 1 ppm
• Lead: ≤ 1 ppm
• Magnesium: ≤ 1 ppm
• Melting Point: 84.0 - 86.0 ^oC
• pH (10%, 0.1 M NaCl @ 25 ^oC) : 6.5 - 7.5
• Solubility (40% Aqueous Solution): Clear and colorless

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PR1MA™ PBS/Tween 20 10X Solution

CAS Number: 7558-79-4, 7778-77-0, 7647-14-5, 7447-40-7, 9005-64-5, 7732-18-5
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: Clear, yellow solution
• pH: 7.2 - 7.6

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PR1MA™ Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.


Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to off white powder
• DNase/ RNase: Not Detected
• EEO: ≤ 0.10 -Mr
• Gelling Temperature: 26 - 30 ^oC
• Gel Strength : ≥ 200 g/cm²
• Melting Temperature: ≤ 65 ^oC
• Moisture Content: ≤ 10 %
• Sulfate Content: ≤ 0.10 %

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PR1MA™ Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.


Add 1 capsule per 500ml of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium Phosphate: 9.4 g/L
• Potassium Phosphate: 2.2 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH (4.76% solution at 25°C): 7.0 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Yeast Extract: 24.0 g/L
• Casein Peptone: 12.0 g/L
• Potassium phosphate dibasic: 9.4 g/L
• Potassium phosphate monobasic: 2.2 g/L
• Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

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PR1MA™ LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.


For propagation and maintenance of E.Coli:

• Add 1 capsule per liter of water.
• Heat and stir until capsule is completely dissolved.
• Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
• Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™  Potassium Phosphate, Dibasic, Anhydrous

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K₂HPO₄
Solubility: Water
Storage Temperature: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 98.0 %
• Chloride: ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Loss on Drying @150 ^oC: ≤ 1.0 %
• Nitrogen Compound: ≤ 0.001 %
• pH (5%): 8.5 - 9.6
• Sodium (Na): ≤ 0.05 %
• Sulfate: ≤ 0.005 %

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PR1MA™  Tetracycline Hydrochloride

CAS Number: 64-75-5
Molecular Weight: 480.9
Chemical Formula: C₂₂H₂₄N₂O₈ • HCl
Solubility: Water
Storage Temperature: Room Temperature


Bacteriostatic antibiotic with activity against gram positive and gram negative bacteria. Slightly soluble in water.

 
WARNING! This product can expose you to Tetracycline HCl, a chemical which is known to the State of California to cause developmental harm. For more information go to www.P65Warnings.ca.gov.

 
Research or further manufacturing use only, not for food or drug use.

• Appearance: Yellow, Crystalline Powder
• Identification (A, B, C): Pass Test
• Specific Rotation: -255 - - 240 °
• Crystallinity: Meets with specification
• pH (1% Solution): 1.8 - 2.8
• Loss on Drying (105 ^oC): ≤ 2.0 %
• Epitetracycline: ≤ 3.0 %
• 4-Ephianhydrotetracyline: ≤ 2.0 %
• Anhydrotetracycline: ≤ 0.5 %
• 2-Acetyl-2-Decarbamoyltetracycline: ≤ 1.5 %
• Chlortetracycline: ≤ 0.5 %
• Sum of impurities other than epitetracycline: ≤ 5.0 %
• Assay of Tetracycline Hydrochloride (On Dried Basis): ≥ 900 µg/mg
• Residual Solvent (1-Butanol): ≤ 5000 ppm
• Residual Solvent (Acetone): ≤ 500 ppm
• Grade: USP
• Country of Origin: Report

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PR1MA™  Cefotaxime, Sodium Salt

CAS Number: 64485-93-4
Molecular Weight: 477.5
Chemical Formula: C₁₆H₁₆N₅NaO₇S ₂
Solubility: Water
Storage Temperature: 2-8°C

Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to B-Lactamase activity.


Non toxic to plant cells
Plant cell culture tested


Research or further manufacturing use only, not for food or drug use.
  

• Appearance: Off White to Pale Yellow Crystalline Powder
• Assay (Potency dry basis) : 916 - 964 µg/mg
• Bacterial Endotoxins : Meet the requirements
• Clarity of Solution: Meet the requirements
• Color of solution: Meet the requirements
• Identification (A, B, C): Pass Test
• Impurity - Individual: ≤ 1.0 %
• Loss on Drying: ≤ 3.0 %
• pH: 4.5 - 6.5
• Specific Rotation: +58 - +64 °
• Total impurities: ≤ 3.0 %

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PR1MA™  Trimethoprim

CAS Number: 738-70-5
Molecular Weight: 290.3
Chemical Formula: C₁₄H₁₈N₄O₃
Solubility: DMSO 50mg/ml, Methanol 12:1 mg/ml, Water 0.4 mg/ml
Storage Temperature: 2-8°C

Inhibits dihydrofolate reductase. Active against most gram negative and gram positive aerobic bacteria.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Off White to Light Yellow Crystalline Powder
• Assay (Anhydrous): 98.5 - 101 %
• Heavy Metals : ≤ 20 ppm
• Loss on Drying: ≤ 1.0 %
• Melting Point: 199 - 203 ^oC
• Related Substances: ≤ 0.2 %
• Sulfated Ash: ≤ 0.1 %

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PR1MA™ SOB Broth

Solubility: Water
Storage Temperature: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Enzymic Hydrolysate: 20 g/L
• Magnesium Sulfate: 2.4 g/L
• Yeast Extract: 5 g/L
• Potassium Chloride: 0.186 g/L
• Sodium Chloride: 0.5 g/L
• Final pH(25°:C): 7.0+0.2

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.8 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Tween 20

Common Name: Polyoxyethylenesorbitan Monolaurate
CAS Number: 9005-64-5
Solubility: Water
Storage Temperature: Room Temperature

Fatty acid composition:

• Lauric acid: Approximately 50%
• Balance: Myristic, Palmitic and Stearic acids.

Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00

Specifications

Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %

Research or further manufacturing use only, not for food or drug use.

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PR1MA™  Gentamicin Sulfate

CAS Number: 1405-41-0
Molecular Weight: 575.67
Chemical Formula: C₂₁H₄₃N₅O₇ • H₂SO₄
Solubility: Water
Storage Temperature: Room Temperature

 
Cell culture tested. Aminoglycoside antibiotic with bactericidal activity against most gram negative bacteria.

 
Research or further manufacturing use only, not for food or drug use.

• Appearance: White to off white powder
• Composition (C₁): 20.0 - 50.0 %
• Composition (C_1a): 10.0 - 35.0 %
• Composition (C_2a + C₂): 25.0 - 55.0 %
• Identification: Meet The Requirements
• Loss on Drying: ≤ 18.0 %
• Methanol: ≤ 1.0 %
• pH: 3.5 - 5.5
• Potency (Dried basis): ≥ 590 µg/mg
• Residual Solvents: Meet Requirements
• Residue on Ignition: ≤ 1.0 %
• Specific Optical Rotation: +107 - +121 °
• Country of Origin : Report