Molecular Biology Reagents/Chemicals

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PR1MA™ Peptone, Powder

CAS Number: 73049-73-7
Solubility: Water
Storage Temperature: Room Temperature

Mixture of peptides and free amino acids from pancreatic hydrolysis of animal tissue. Low NaCl content suitable for tissue culture.


Total Nitrogen (TN): 15.0-16.0%
Amino Nitrogen (AN): 3.0-3.5%
AN/TN Ratio: 0.21-0.23


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Light Beige Fine Powder
• Amino Nitrogen: ≥ 3.4 %
• Ash, Residue in Ignition: ≤ 15 %
• Loss on Drying: ≤ 5 %
• Nitrites: Negative
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Proteoses: Report
• Total Nitrogen: ≥ 10 %
• Undigested Protein: Negative
• Coliforms: Negative
• E.Coli: Negative
• Molds and Yeasts: Report cfu/g
• Salmonella : Negative cfu/25g
• Standard Plate Count : ≤ 1000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Raw Material Species: Bovine
• Enzyme Species: Porcine

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PR1MA™ Glycine, Free Base

CAS Number: 56-40-6
Molecular Weight: 75.1
Chemical Formula: C₂H₅NO₂
Solubility: Water
Storage Temperature: Room Temperature

For use in preparing electrophoresis buffers. Cell culture tested.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White odorless crystalline powder with sweet taste.
• Solubility (5% in water): Clear & colorless
• Identification (IR): Test Passes
• Loss on Drying (@105 ^oC for 2 hrs): ≤ 0.20 %
• Residue on Ignition: ≤ 0.10 %
• Chloride (Cl): ≤ 0.007 %
• Sulfate: ≤ 0.0065 %
• Heavy Metals: ≤ 0.002 %
• Hydrolyzable Substances: Test Passes
• Related Compound (Diglycine): ≤ 0.1 %
• Related Compound (Triglycine): ≤ 0.1 %
• Related Compound (Glycine Anhydride): ≤ 0.1 %
• Related Compound (Monochloro Acetic Acid): ≤ 0.1 %
• Related Compound (Iminodiacetic Acid): ≤ 0.1 %
• Related Compound (Haxamathylenetetraamine): ≤ 0.1 %
• Related Compound (Total Impurities): ≤ 1.0 %
• Residual Solvent (Chloroform): ≤ 60 mcg/gm
• Residual Solvent (1,4 Dioxane): ≤ 380 mcg/gm
• Residual Solvent (Methylene Chloride): ≤ 600 mcg/gm
• Residual Solvent (Trichloroethylene): ≤ 80 mcg/gm
• Methanol: ≤ 0.3 %
• Assay: 98.5 - 101.5 %
• Arsenic: ≤ 1.0 ppm
• Total Viable Bacterial Count: ≤ 1000 Cfu/gm
• Coliform Count: Negative
• Yeast and Mold Count: ≤ 100 CFU/g
• Test for E-Coli: Absent
• Test for Salmonella: Absent
• Test for S-Aureus: Absent
• Test for Pseudoomonas: Absent
• BSE/TSE Free: BSE/TSE Free
• Grade: USP
• Country of Origin : Report

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PR1MA™  Sulfamethoxazole

CAS Number: 723-46-6
Molecular Weight: 253.3
Chemical Formula: C₁₀H₁₁N₃O₃S
Solubility: Acetone
Storage Temperature: Room Temperature

Bacteriostatic antibiotic that inhibits nucleic acid synthesis in sensitive micro-organisms.


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Off White Crystalline Powder
• Infrared Spectrum: Conforms to Structure
• Loss on Drying: ≤ 0.5 %
• Residue on Ignition: ≤ 0.1 %
• Related Substance (Sulfanilic acid): ≤ 0.10 %
• Related Substance (Sulfanilamide): ≤ 0.10 %
• Related Substance (Impurity C, A): ≤ 0.10 %
• Related Substance (Impurity B): ≤ 0.10 %
• Related Substance (Impurity F): ≤ 0.10 %
• Any other Impurity: ≤ 0.10 %
• Total impurities: ≤ 0.30 %
• Assay (Titration): 99.0 - 101.0 %
• Grade: USP
• Country of Origin : Report

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PR1MA™  Validamycin A

CAS Number: 37248-47-8
Molecular Weight: 497.5
Chemical Formula: C₂₀H₃₅NO₁₃
Solubility: DMSO, slightly soluble in ethanol
Storage Temperature: 2-8°C

Inhibits trehalase activity. Enhances trehalose accumulation in transgenic plants.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light yellow loose powder
• Assay: ≥ 64.0 %
• pH: 2.5 - 5.0
• Water: ≤ 5.0 %
• Water Insoluble: ≤ 0.2 %

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PR1MA™  Timentin [Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio]

Common Name: Ticarcillin Disodium Salt/Potassium Clavulanate Mixture 15:1 Ratio
CAS Number: 4697-14-7; 61177-45-5
Molecular Weight: 428.39 / 665.7
Chemical Formula: C₁₅H₁₄N₂Na₂O ₆S₂ / C₈H₈KNO₅
Solubility: Water
Storage Temperature: 2-8°C

Highly effective combination against gram negative bacteria such as Agrobacterium strains. -Lactamase inhibitor.


Plant cell culture tested. Hygroscopic.


Mixture Contains:

• 1875 mg Ticarcillin to 125 mg potassium clavulanate (15:1 ratio)

• Appearance: White to Pale Yellow Crystalline Powder
• Assay (Clavulanic Acid): ≥ 5.0 %
• Assay (Ticarcillin): ≥ 75.0 %
• Bacterial Endotoxins : ≤ 0.07 EU/mg
• Identification: Conform
• Particulate Matter: Conforms
• pH: 5.5 - 7.5
• Potency (Clavulanic Acid): ≥ 50 µg/mg
• Potency Ticarcillin: ≥ 750 µg/mg
• Proportion of Ticarcillin to Clavulanic acid: 14:1 - 16:1
• Related Substances (Largest Impurity): ≤ 4.0 %
• Related Substances (Second Major Impurity) : ≤ 2.5 %
• Water (KF): ≤ 4.5 %

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PR1MA D-(+)-Glucose [Dextrose Anhydrous]

• Common Name: Dextrose Anhydrous
• CAS Number: 50-99-7
• Molecular Weight: 180.16
• Chemical Formula: C₆H₁₂O₆
• Solubility: Water
• Storage Temperature: Room Temperature

USP Grade material.

Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: White Crystalline Powder
• Assay: 97.5 - 102.0 %
• Identification (FTIR Spectrum): Pass Test
• Identification (HPLC): Matches the reference
• Specific Rotation: +52.6 - +53.2 °
• Moisture: ≤ 1.0 %
• Residue on Ignition: ≤ 0.1 %
• Color of solution: Qualified
• Conductivity: ≤ 20.0 µs/cm
• Dextrins: Qualified
• Soluble starch and sulfite: Qualified
• Related Substance ( Maltitol and Isomalt): ≤ 0.4 %
• Related Substance (Maltotriose): ≤ 0.2 %
• Related Substance (Fructose): ≤ 0.15 %
• Related Substance ( Unspecified Impurities): ≤ 0.1 %
• Related Substance (Total impurity): ≤ 0.5 %
• Heavy Metals: ≤ 5 ppm
• Residual Solvents: None
• Total Bacterial Count: ≤ 1000 cfu/g
• Mold and Yeast: ≤ 100 cfu/g
• E.Coli: Negative
• Endotoxin: ≤ 1.25 EU/g
• Source: Corn
• Grade: Meets the current USP Grade
• BSE/TSE Free: BSE/TSE Free
• Country of Origin: Report

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PR1MA™ X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside]

Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-galactoside
CAS Number: 7240-90-6
Molecular Weight: 408.6
Chemical Formula: C₁₄H₁₅BrCINO₆
Solubility: DMSO and DMF
Storage Temperature: -20°C

Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.


Water Content: 0.05% (K. Fisher)
Protect from light and moisture


Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Identity: Conforms to structure
• Purity (HPLC): ≥ 98.0 %
• Solubility (2%, DMF): Clear and Colorless Solution
• Specific Rotation: -63.0 - -60.0 °
• Water Content (K.F): ≤ 0.5 %

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PR1MA™  Carbenicillin, Disodium Salt

CAS Number: 4800-94-6
Molecular Weight: 422.4
Chemical Formula: C₁₇H₁₆N₂Na₂O ₆S
Solubility: Water
Storage Temperature: 2-8°C

USP grade

Prevents bacterial cell wall synthesis. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. Sensitive to B-Lactamase.
 

• Non-toxic to plant cells
• Plant cell culture tested
• Hygroscopic

Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to Off White Crystalline Powder
• Identification, Sodium Reaction: Pass Test
• Water: ≤ 6.0 %
• pH: 6.50 - 8.0
• Clarity of Solution: Clear Solution
• Benzyl penicillin sodium: ≤ 5.0 %
• Chromatographic Purity (HPLC): ≥ 92.0 %
• Assay (Anhydrous): ≥ 88.0 %
• Bacterial Endotoxins : ≤ 0.05 EU/mg
• Iodine Absorbing Substances: ≤ 8.0 %
• Potency (Dried basis): ≥ 770 µg/mg
• Grade: USP
• Country of Origin : Report

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PR1MA™  Mitomycin C

CAS Number: 50-07-7
Molecular Weight: 334.3
Chemical Formula: C₁₅H₁₈N₄O₅
Solubility: Ethanol
Storage Temperature: 2-8°C
 

Antineoplastic antibiotic that acts as an alkylating agent after activation and also suppresses the synthesis of nucleic acids. A cell-cycle non specific agent, most active in the late G1 and early S phases.


WARNING! This product can expose you to Mitomycin-C, a chemical which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.
 
 

• Appearance: Blue violet solid
• Identification (UV): Conforms
• Purity-HPLC (RP CH₃CN:H₂O-0-100%): ≥ 99.0 %
• Purity-HPLC (RP MEOH:H₂O-90-10): ≥ 98.0 %
• Purity-TLC (CH₂CL₂:MEOH- 9:1): ≥ 98.0 %
• Solubility (10 mg/ml MEOH): Clear Blue Solution

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PR1MA™  MOPS [3-(N-morpholino)-propanesulfonic acid]

Common Name: 3-(N-Morpholino)propanesulfonic acid
CAS Number: 1132-61-2
Molecular Weight: 209.3
Chemical Formula: C₇H₁₅NO₄S
Solubility: Water
Storage Temperature: Room Temperature

Molecular Biology Grade

• Heavy Metals: <5 ppm
• pKa: (20°C) 7.20 ± 0.2
• pH Range: 6.5-7.9

• Appearance: White crystalline powder
• Absorbance @250nm, 1M solution in H₂O: ≤ 0.1
• Absorbance 260nm: ≤ 0.2 nm
• Absorbance 280nm: ≤ 0.2
• Assay (Titration): ≥ 99.0 %
• Endotoxin : ≤ 0.5 EU/mg
• Heavy Metals : ≤ 5 ppm
• Identification: Matches the reference
• Iron (Fe): ≤ 5 ppm
• Loss on Drying (105 ^oC): ≤ 1.0 %
• Melting Point : 276.0 - 281.0 ^oC
• pH: 2.5 - 5.0
• pKa @ 20°C: 7.0 - 7.4
• Residue on Ignition: ≤ 0.2 %
• Solubility: Clear, colorless solution

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PR1MA™ Acrylamide, 40% Solution

CAS Number: 79-06-1; 7732-18-5
Storage Temperature: 2-8°C

A 40% (w/v) Acrylamide solution.


WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear, Colorless Solution
• Acrylic Acid: ≤ 0.003 %
• Conductivity: ≤ 5 µmhos
• DNase, RNase, protease: Not Detected
• Electrophoresis: Pass Test
• pH @25 °C: 6.0 - 7.0

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PR1MA™ Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temperature: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.


Suitable for Microbiology Applications.


Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Powder
• Amino Nitrogen Content: ≥ 3.5 %
• Dry Matter: ≥ 94 %
• Moisture : ≤ 6.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen Content: ≥ 8.0 %
• Non Animal Origin: The product does not come in contact with materials of animal origin.