PR1MA Hot Start Taq

• Exceptional sensitivity for low copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility

Engineered for controlled polymerase activity, PR1MA Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye.  The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

• View DNA migration and separation as it happens
• Blue light illumination - won't damage DNA
• Capture high resolution images without removing the gel from the tank

The myGel InstaView comes complete with everything needed to cast, run, and view mini electrophoresis gels, including an integrated power supply.

Gels are easily poured using the supplied trays, combs and leak-proof casting stand.  One large (10.5x10cm) or one small (10.5x6cm) gel is accommodated by the gel tank.

The power supply connects directly into the gel tank.  With no more leads to contend with the connection is guaranteed to be right, every time.  Output voltage can be adjusted to 35V, 50V, or 100V and run time can be set for up to 99 minutes.  If the lid is not properly in place a magnetic interlock between the gel tank and the lid prevents current from running through the system.

Once the system is set up, slide the mini LED transilluminator into place under the tank and at any time turn it on to view the run in real time.  An array of blue LEDs emits bright blue light at a wavelength of 465nm for optimal excitation of common green fluorescent stains such as SmartGlow, SYBYR Green and Gel Green, without damaging DNA.  An orange filter is incorporated into the lid for visualizing DNA bands eliminating the need for additional equipment or special glasses.

To document results, place the included gel imaging enclosure over top of the myGel InstaView and use the camera ap on any smart phone to capture high resolution images without removing the gel from the tank.  Images can easily be shared via email, text, or printed.

Technical Data

Output voltage	35V, 50V or 100V
Timer	0-99 mins, continuous
Safety	Magnetic switch
Buffer Volume	230ml
Gel Capacity	1-10.5x10cm or 1-10.5x6cm gel, 0.5cm thick
Transilluminator	Blue LED, 465nm
WxDxH (w/enclosure)	10x8.25x5in/25.5x21x14cm
Weight	2.3lb/1.04kg
Electrical	115V, 60Hz or 230V, 50Hz
Warranty	2-year

InstaView Gel Tank

The InstaView Gel Tank is the perfect choice for labs that prefer to use a separate power supply.  The InstaView Gel Tank accommodates one large (10.5x10cm) or one small (10.5x6cm) gel tray and is supplied with trays, combs and a leak-proof casting stand. The gel tank lid includes attached power leads which prevent the unit from operating without the lid properly in place. Any standard power supply can be connected, including the Accuris myVolt Mini and myVolt Touch.

Technical Data

Safety	Physical interlock between lid and tank
Buffer Volume	230ml
Gel Capacity	1-10.5x10cm or 1-10.5x6cm gels, 0.5cm thick
Transilluminator	Blue LED, 465nm
WxDxH (w/enclosure)	7.5x8.25x5in /19x21x14cm
Weight	1.65lb/0.75kg
Electrical	115V, 60Hz or 230V, 50Hz
Warranty	2-year

System & Accessories:

E1201  myGel InstaView Electrophoresis System, includes gel tank with orange filter, direct connect power supply, E1200-CS1 casting set (see below), Mini SmartBlue Transilluminator and imaging enclosure

E1200 - InstaView Gel Tank, includes tank with orange filter, C1200-CS1 casting set (see below), mini UV
transilluminator and imaging enclosure (power supply not included)

E1200-CS1 - Additional casting set, includes casting stand, divider, 2 trays for 10.5x6cm gels, one tray for 10.5x10cm gels, 2 double-sided combs -12/22 teeth

E1101-COMB1 - Additional double-sided combs, 12/22 teeth, pack of 2

PR1MA High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers

For applications requiring highly accurate amplification, choose PR1MA High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

 

Item	Description	Volume
PR1000-HF-S	PR1MA High Fidelity DNA Polymerase	20 Units (Sample)
PR1000-HF-200	PR1MA High Fidelity DNA Polymerase	200 Units (2 U/p1)
PR1000-HF-500	PR1MA High Fidelity DNA Polymerase	500 Units (2 U/p1)
PR1000-HF-1000	PR1MA High Fidelity DNA Polymerase	1,000 Units (2 U/p1)

StripSpin 12 Mini Centrifuge

• Unique rotor for 12-strip PCR tubes
• Also can be used with 8-strip and individual PCR tubes
• Starts and Stops with opening/closing the lid
• Tiny benchtop footprint and affordability
• Place a StripSpin at the bench, thermal cycler & water bath for convenience.

Specifications:

Speed:	5,500 rpm / 2,000 xg
	4 x 12 position PCR strips
Capacity:	4 x 8 position PCR strips
	48 x 0.2ml PCR tubes
Dimensions:	5.5 x 7.9 x 4.4 in.
(w x d x h)	14 x 20 x 11.2 cm
Weight:	2.2 lbs. / 1 kg
Electrical:	Universal Voltage
	100-240V, 50-60 Hz, 300W
Warranty:	1 Year

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets

PR1MA High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, an antibody mediated hot start mechanism, and a proof-reading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

• Dimensional uniformity for superior thermal transfer
• AVST-01 flat caps are optically clear and ideal for qPCR
• Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

Please give us a call for a sample!

PR1MA High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

Pryme PCR Standard Individual Tubes 0.2 & 0.5 mL

Thinwall flattop or domed capped with attached tubes are available in 0.2 mL and 0.5 mL volumes.   0.2 mL tubes are suitable for use in all thermal cyclers with standard 0.2 mL block formats.  0.5 mL tubes are suitable for use in all thermal cyclers with standard 0.5 mL block formats.  Available in clear tubes, individual colors, and assorted colors.  The thin wall feature accelerates heat transfer, providing shorter cycle times.

PR1MA qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

PR1MA qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and reverse transcriptase. Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  PR1MA Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95°C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

qMAX™ Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

**New Name, Same Great Product!**

PR1MA SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details

Protocol:  Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration:  0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage:  SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production:  SmartCheck™ ladders are produced from proprietary plasmids, digested to completion.  A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.
 
 

Item	Description	Volume
PR4005-100	PR1MA SmartCheck™ 50bp DNA Ladder	500 µL / 100 Lanes
PR4005-500	PR1MA SmartCheck™ 50bp DNA Ladder	5 x 500 µL / 500 Lanes
PR4010-100	PR1MA SmartCheck™ 100bp DNA Ladder	500 µL / 100 Lanes
PR4010-500	PR1MA SmartCheck™ 100bp DNA Ladder	5 x 500µL / 500 Lanes
PR4100-100	PR1MA SmartCheck™ 1kb DNA Ladder	500 µL / 100 Lanes
PR4100-500	PR1MA SmartCheck™ 1kb DNA Ladder	5 x 500 µL / 500 Lanes

PR1MA Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

Item	Description	Volume
PR1300-MG-S	PR1MA 1 Hour Mammalian Genotyping Kit	8 Reactions (Sample)
PR1300-MG-80	PR1MA 1 Hour Mammalian Genotyping Kit	80 Reactions
PR1300-MG-400	PR1MA 1 Hour Mammalian Genotyping Kit	400 Reactions
PR1300-MG-800	PR1MA 1 Hour Mammalian Genotyping Kit	800 Reactions

 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

Item	Description	Volume
PR1301-HSR-400	PR1MA Genotyping Hot Start Master Mix, 2X Conc., Red Dye	400 Reactions