• Designed for convenience & versatility, this rack can be used while you are working or as a storage rack.
• It includes a lid and can accomodate 32 x 0.2 ml PCR tubes, 24 x 1.5/2.0 ml & 16 x 0.5 ml microtubes.
• Tube wells are alpha-numerically matrixed with raised bold type for quick identification.
  
• If you use cycle plates, 8 or 12 tube strips, or lage quantities of 0.2 ml PCR tubes, the WRI-4A is for you.
• Four retainer clips hold the tray firmly in place, yet allow fast and convenient removal when necessary.
• Available with a clear lid and five flourescent colors: blue, green, orange, pink, yellow, or assorted.

MyGo Mini S

• Small format 16 x 0.1 mL QPCR System with Taqman Multiplexing at an amazing price.
• Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and analyze the data at the same time.  Windows, Linux and Mac OSX
• Solid state reliability with up to 5-year warranty

Precision and Performance
The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.

Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.

For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.
 

Easy Analysis
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.

Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
 

Specifications

MyGo Pro

• Small format 32 x 0.1 mL QPCR System, holds four 8 x 0.1 mL Strips.
• Runs 45 cycles in 33 minutes.
• Amazing accuracy and easy to use software that allows you to control 100s of systems with one PC and Analyze the data at the same time.  Windows, Linux and Mac OSX.
• Solid state reliability with option of 5-year warranty.

Precision and Performance
The MyGo MPro demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis.

Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations.

For users performing HRM-based analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini S system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM.

Easy Analysis
The MyGo Pro system comes with easy-to-use software, and robust, high performance algorithms provide accurate results from complex data. Automated analysis of complex HRM data makes this
powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems.
 

Specifications

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

Our rack is perfect for pre- or post-PCR® sampling applications. Sturdy polypropylene rack accommodates individual 0.2mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with imprinted numbers and letters. Features a removable hinged lid. Available in natural, blue, green, pink, yellow, or assorted colors (one of each). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.

PR1MA One-Step RT-PCR Kit

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

PR1MA qMAX™ cDNA Synthesis Kits

PR1MA now offers two cDNA Synthesis Kits, to meet a range of requirements.

The original cDNA Synthesis Kit is a 2-tube format for easy reaction set up and is ideal for 4 pg to 0.5 µg of input RNA.  One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 

The 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers.  This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
 
 
 
PR1MA qMax cDNA Synthesis Kits

PR1MA qMax First Strand cDNA Synthesis Flex Kits 

Designed for laboratories with medium to high throughput needs, the TC9639 Thermal Cycler provides fast, reliable results. Featuring a large, touch screen interface, a next-generation program wizard and a unique multi-format block, this unit makes it easier than ever to get up and running.

A large, full color touch screen serves as the operations center where all functions are accessed. Starting at the "Home" screen, simply touch an icon to view the last run, enter the program library or use the program wizard to quickly enter a new protocol.

Designed with extensive user feedback, the program wizard quickly and easily guides users through protocol set-up, all in a single screen. Time and temperature increments can be built into programs as well as slower ramp rates. Storage capabilities are unlimited; programs can be stored in public folders, password protected folders or on a flash drive.

The fully adjustable heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Heating and cooling of the block are precisely and accurately controlled within exceptionally tight tolerances by a proprietary algorithm that simulates sample temperature.

With the multi-format block, there is no need for the expense of multiple interchangeable blocks. The TC 9639 has the flexibility to accept 96 x 0.2ml tubes, a 96 well plate or 39 x 0.5ml tubes, all in the same sample block. An in-situ adapter is available for use with slides. A 384 well version is available.

• Gradient capabilities- ideal for protocol optimization
• Intuitive touch screen control- Works even when wearing gloves!
• Upgraded software- Even easier to use than before!
• No need for interchangeable blocks- multi-format accepts:
• 0.2ml tubes or strips (96)
• 0.5ml tubes (39)
• 96 well PCR plate

PR1MA dNTPs

• Supplied as a ready-to-use 40mM mix or a set of 4 separate 100mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
 
 
 
PR1MA dNTP Mix

PR1MA dNTP Set 

Mystaire® CleanPrep™ dead air boxes are circulation-free enclosures that establish an ideal work area to protect against contamination in sensitive PCR amplification reactions. CleanPrep™ workstations are constructed from thermally-fused polypropylene that offers exceptional chemical resistance, does not adsorb moisture and can be easily cleaned and decontaminated between amplifications using a variety of disinfectant solutions.Seamless polypropylene design for easy decontamination between amplifications.

FEATURES:

1. Clear polycarbonate access with overlap and safety position switch
2. UV shelf with integrated pipette holder - accommodates four pipettors
3. High intensity fluorescent lamps and shortwave UV light
4. Available in 36 and 48 inch widths with nominal 24 inch depth
5. Circulation-free enclosure for PCR applications

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.