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Need a great DNA Ladder at a great price? Try PR1MA!  Click here to order.


A unique combination of PCR products and digested proprietary plasmids for agarose gel electrophoresis.

  • A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
  • The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
  • The approximate mass of DNA in each band is provided (0.5µg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

  • PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 µg/mL
Package: 50 µg/500 µL
Recommended Load: 5 µL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)
Storage:

  • Store at 25°C for 6 months
  • Store at 4°C for 12 months

Store at -20°C for 24 months

Item#:
ASDNALADD4

Need a great DNA Ladder at a great price? Try PR1MA!  Click here to order.

Bullseye 100bp DNA Ladder

  • Ready to use
  • Contains 11 DNA bands: 100-1500bp.
  • Clearly identifiable 500bp band as reference
  • 500ng DNA/6µl/loading
  • Easy to load
  • Stable at room temperature
  • Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6µl will yield at least 30ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.


Size: 1200µl
Storage: Store at -20°C.
Concentration: 500ng/6µl
Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add at least 6µl Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6µl of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

Item#:
ASDNALADD1

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:
Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT1

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT4
  • Sample: Gram (+) positive and Gram (-) negative bacterial cells
  • Yield: Up to 30 µg of gDNA - (1 x 10 Escherichia coli: 25-30 µg, 1 x 10 Bacillus subtilis: 10-15 µg)
  • Convenient: Includes Gram+ Buffer for preparing lysozyme solutions and to speed up sample preparation
  • Format: Genomic DNA spin columns
  • Operation Time: within 30 minutes
  • Elution Volume: 50-200 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

The gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

Quality Control
The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (1x10) culture (OD600=1.3, 1 mL) harvested by centrifugation at 16,000 x g for 1 minute. 10 µL from a 50 µL eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Items

IB47290

IB47291

IB47292

Gram+ Buffer*

1.5 mL

30 mL

75 mL

GT Buffer

1.5 mL

30 mL

75 mL

GB Buffer

2 mL

40 mL

100 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer**
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL

50 mL
(200 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add lysozyme to Gram+ Buffer immediately prior to use. Once lysozyme is mixed with Gram+ Buffer, the solution can be stored for 2 weeks at 4°C.


**Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

Item#:
ASDNARNAKIT3

The Large DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

Sample agarose gel
Recovery up to 85%
Format spin column
Operation time 45 minutes
Item#:
ASDNARNAKIT9

Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
ASDNARNAKIT6

Advantages

  • Sample: Tissue, rodent tails, ear punches, fresh or frozen blood, serum, plasma, buffy coat, body fluids, cultured cells, amniotic fluid, FFPE, hair, insects
  • Yield: Up to 5 µg of gDNA from fresh whole blood samples
  • Format: genomic DNA spin column
  • Operation Time: Within 20 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year

Introduction
The gMax Mini Kit is optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), tissue, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

Quality Control
The quality of the gMax Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 μL of whole human blood. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Kit Components


Component

IB47280

IB47281

IB47282

GST Buffer

3 mL

30 mL

75 mL

GSB Buffer

4 mL

40 mL

75 mL

W1 Buffer

2 mL

45 mL

130 mL

Wash Buffer*
(Add Ethanol)

1 mL
(4 mL)

25 mL
(100 mL)

50 mL
(200 mL)

Proteinase K**
(Add ddH2O)

1 mg
(0.10 mL)

11 mg x 2
(1.10 mL)

65 mg
(6.50 mL)

Elution Buffer

1 mL

30 mL

75 mL

GD Columns

4

100

300

2 mL Collection Tubes

8

200

600

*Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.

**Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure Proteinase K is completely dissolved. Check the box on the bottle. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4°C.

Item#:
ASDNARNAKIT5

REPLACEMENT GD COLUMNS & COLLECTION TUBES - 100 PACK

  • For use with leftover reagents from IBI Genomic DNA Kits (Tissue, Blood & Cultured Cells) or competitive products
  • Sample Size: 300 mL fresh whole blood/107 animal cultured cells
  • Elution Volume: 50 to 200 µL
  • Binding Capacity: Up to 10 µg

The Replacement Genomic DNA Columns & Collection Tubes are for use with leftover reagents from IBI Genomic DNA Kits including the following item numbers: IB47201, IB47202, IB47211, IB47221, IB47222, IB47231, IB47241, IB47242, IB47281, IB47282, IB47291, and IB47292.

The columns and collection tubes can also be used with comparable, competitive Genomic DNA Kit reagents that utilize a lysis, bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

Item#:
ASIB47207
 
Protocal
Sample Kit, 4 preps

Isolation of micro RNA (miRNA) from Tissue or Cultured Cells

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Sample Types:
100mg tissue or 1 x 10 cultured cells
Format:
Spin column

Operation Time:
30 minutes

Item#:
IB47370
Your Price:
25.00
Each
Kit, 100 preps

Isolation of micro RNA (miRNA) from Tissue or Cultured Cells

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Sample Types:
100mg tissue or 1 x 10 cultured cells
Format:
Spin column

Operation Time:
30 minutes

Item#:
IB47371
Your Price:
428.52
Each
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