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We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
MIDSCI PCR 8-Strip Caps are compatible with MIDSCI PCR plates. The caps are available as a dome caps (AVT3878) or flat caps (AVT3799).
Specifications of MIDSCI strip caps:
- Highest standards of quality control
- Lot to Lot consistency
- Certified DNase/RNase, nucleic acid, PCR-inhibitor and pyrogen-free
- See fit chart for plate compatibility
PCR Work-Up Rack fits PCR 0.2ml tubes, strip tubes or 96-well plates (including skirted plates). Polypropylene rack and lid click firmly for secure transportation and storage. Available in assorted colors: blue, green, yellow, violet and red.
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
- Non-Skirt, low-profile (0.1ml)
- Cuttable into 16, 24, 32, or 48-well pieces
- White for optimal qPCR signal/noise ratios
- Thin-wall for consistent and accurate heat transfer
- Ultra-low sample evaporation
- Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE
Closure can be accomplished with B57801 optical indented strip caps.
Click here to Order Now!
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted, cuttable
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Made from 99.9% virgin polypropylene
- Single notch corner
- 335ul well capacity (working capacity dependent on closure method)
- Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
- Certified DNAse/RNAse and pyrogen-safe
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
- Optimized for use with all of IBI 96-well kits
- The thin upper portion of the manifold is designed to reduce cross contamination
- Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
- Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
- Dimensions: 17x12x8cm
- Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
Bullseye 1Kb DNA Ladder, Logic
- Ready to use
- Contains 16 DNA bands: 100bp-10Kb
- 920ng DNA/6µl/loading
- Easy quantification of DNA fragments
- Stable at room temperature
- Supplied with 6x sample loading buffer
Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.
Size: 1200µl
Storage: Store at -20°C.
Concentration: 920ng/6µl
Loading Buffer Composition:
10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol
Usage: Add 6µl of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6µl of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.