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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
- Fast ramping - up to 5°C/second
- Intuitive graphical programming
- Lightweight and compact
- Interchangeable blocks - 24 x 0.2 ml and 18 x 0.5 ml
- Economical - half the price of a full size unit
- 18 month warranty
Product Information | ||
Labnet's MultiGene Mini is a compact and lightweight thermal cycler, but don't let its small size fool you. This cycler is packed with features typically found only on larger thermal cyclers. | ||
Excellent accuracy and uniformity combine with fast ramping rates to provide quality results. An algorithm calculates sample temperature, based on Vol, to control heating and cooling of the block. This means that samples reach programmed temperatures quickly, without any overshoot or lagging. Powerful Peltier units provide ramping rates as fast as 5°C/second. | ||
Programming the MultiGene Mini is simple and intuitive. The control pad combines function keys, a key pad and arrow keys for easy navigation and entering of parameters. The large graphical display is easy to read. In addition to the standard parameters of time and temperature, the software also allows for successive time and temperature increments and decrements (for touchdown amplification and auto-extension), auto-restart after a power failure, end of cycling elongation steps and extended soaks at 4°C. One hundred programs, each with up to nine segments, can be stored in memory. | ||
The MultiGene Mini is available with either a 24 x 0.2 ml tube block or 18 x 0.5 ml tube block. Blocks are easily interchanged. Units come standard with an adjustable heated lid. The lid features a slip gear to provide proper compression to prevent sample evaporation without deforming the tubes. | ||
Product Specifications | ||
Capacity | ||
TC020-24 | 24 x 0.2 mL tubes | |
TC050-18 | 18 x 0.5 mL tubes | |
Programmable temp. range | 4° to 99°C | |
Temp. accuracy/uniformity | ±0.3°C/±0.5°C at 55°C | |
Max. heating/cooling rate | 5°C/4°C per second | |
Heating/cooling method | Peltier | |
Heated lid temperature | 105°C | |
Program memory | 100 programs | |
Max. cycles/segments | 99 cycles/9 segments | |
Max. hold time/step | 99 minutes 59 seconds | |
Dimensions (WxDxH) | 8.6 x 11.2 x 7 in / 21.8 x 28.5 x 17.8 cm | |
Weight | 7.1 lb/3.2 kg | |
Electrical | 120V/230V, 50/60 Hz |
AeraSeal™ features a hydrophobic, gas permeable membrane with a medical grade, non-cytotoxic adhesive. The porous membrane consists of layers of heat welded rayon fibers.
- Pore size from 10 µm to 50 µm
- Moisture vapor transmission rate = 4,200 g/m²/24 hr
- Easy-to-pierce with serological pipets and pipet tips for direct sample recovery
- Available in roll format for automation: AeraSeal™ Roll-Seal
- Cell culture
- Tissue culture
- Bacterial cultures
- -20°C to +80°C
SealPlate® SealMate™ Rolls for Fast and Efficient Microplate Sealing
The SealMate SealPlate Starter Kit features a re-usable dispenser and two rolls of perforated film.
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes film contamination.
- Environmentally friendly; no paper liner to remove and discard; utilizes approximately 72% less material than SealPlate sheeted film
- Each sheet is separated by an adhesive-free, perforated, color-coded green band
- The smooth lower surface of the dispenser can be used to seal the film to the plate
- Dispenser protects unused seals from contamination
- Lower support prop prevents dispensing bill from contacting surfaces to prevent contamination
- Replacement rolls can be easily installed by releasing the locking clasps on the dispenser
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area.
Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.
Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area.
Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.
Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area.
Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.
IBI DEPC-Treated, Nuclease-Free Water is ideally suited for all applications in molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and specifically for all RNA assays.
IBI DEPC-Treated Water is manufactured under stringent conditions. The purification process for this product includes continuous
deionization, reverse osmosis, UV-treatment, 0.1µm filtration, followed by steam sterilization in an autoclave. The product is re-autoclaved
to ensure the breakdown of DEPC and release of carbon dioxide that may cause the pH to shift.
IB42200 |
DEPC Treated Water, nuclease free, 125mL |
IB42201 |
DEPC Treated Water, nuclease free, 24 x 125mL |
IB42202 |
DEPC Treated Water, nuclease free, 48 x 125 mL |
IB42210 |
DEPC Treated Water, nuclease free, 500 mL |
IB42211 |
DEPC Treated Water, nuclease free, 10 x 500 mL |
IB42212 |
DEPC Treated Water, nuclease free, 20 x 500 mL |
IB42220 |
DEPC Treated Water, nuclease free, 1L |
IB42221 |
DEPC Treated Water, nuclease free, 6 x 1L |
IB42222 |
DEPC Treated Water, nuclease free, 12 x 1L |
IB42230 |
DEPC Treated Water, nuclease free, 2L |
IB42231 |
DEPC Treated Water, nuclease free, 6 x 2L |
IB42240 |
DEPC Treated Water, nuclease free, 10L |
- Tracks the migration progression of your sample during polyacrylamide electrophoresis
- Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
- DNase/RNase/Protease free
PR1MA™ LD032 Mini Thermal Cycler
- "Mini" sized, 9 x 7 inch footprint
- Capacity for 32 x 0.2 mL or 4 PCR Strips
- Intuitive touch screen control
"Mini sized" doesn't have to mean a sacrifice on features and performance. PR1MA's™ LD032 Mini Thermal Cycler offers advanced programming features, easy to use software and fast, reliable cycling but with a smaller block and lower price point.
Designed for lower throughput needs, the LD032 Mini Thermal Cycler has a capacity for 32 x 0.2 mL tubes. The wells are arranged in a 4 x 8 format, allowing the block to accept strips of 8 as well as individual tubes. The block heats and cools very quickly - at rates as fast as 5°C/second. A proprietary algorithm controls block temperature, bringing samples quickly to the target temperature without any over- or under-shoot. Excellent block uniformity ensures consistency sample to sample.
Operation of the thermal cycler is controlled from the large, full color, touch screen. Icon driven menus allow users to get running quickly. Programming is extremely simple and includes options for time and temperature increment/decrements, adjustable ramp rates, and an auto pause and restart functions. More than 100 typical programs can be stored in memory and easily recalled. After a run, samples can be held at 4°C. An on-screen report is also available at the completion of a run.
The heated lid is self-adjusting - the spring loaded platen in the lid comes firmly into contact with the tops of the tubes to prevent sample evaporation and condensation. There are no knobs to turn or levers to adjust. The temperature of the heated lid can be changed, or the heating function of the lid may be turned off.
With a footprint less than that of the average laboratory notebook, the LD032 Mini Thermal Cycler will fit almost anywhere. Its reduced capacity block and economical price make the TC-32 Mini Thermal Cycler the perfect choice for lower throughput laboratories and educational facilities.
Specifications
Capacity - 0.2ml tubes |
32 |
Capacity - 8 x 0.2ml PCR strips |
4 |
Temp. Control Range |
0° to 99°C |
Temp. Control |
Simulated sample or block |
Accuracy/Uniformity |
± 0.25°C | ± 0.25°C |
Ramp Rate |
Heating: 5°C / Sec. (max) Cooling: 5°C / Sec. (max) |
Lid Temp |
30°C to 112°C, user adjustable |
Program Memory |
Over 100 typical programs |
Increments/Decrements |
Yes, both time and temperature |
Power Failure Restart |
Yes |
Power Supply |
Universal,100 to 240 V, 50 to 60 Hz |
Dimensions |
7.2 x 9.2 x 6.2 in. | 18.2 x 23.2 x 15.7 cm |
Weight |
6.4 lbs. | 2.9 kg |
Warranty |
2 years |
General Purpose Sealing Film For a More Secure Seal to Minimize Evaporation
General Purpose polypropylene films offer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.- Certified DNase- & RNase- free
- Features good chemical resistance
- Provides a very secure seal to minimize evaporation, but is slightly harder to remove than SealPlate films
- Less rigid polypropylene film reduces aerosols upon removal
- Specially formulated adhesive minimizes plate residue
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
- Safely excise bands from gels
- Avoid cross contamination
- One-handed operation for quick and accurate gel cutting
- Safer than using razor blades and won't scratch transilluminators
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
Combo Includes:
200 μL Taq DNA Polymerase (5 units/μL)
4 x 500 μL vials of dNTP Premix (100 mM each of dATP, dCTP, dGTP, dTTP)
D118-1000
High-performance Taq DNA Polymerase specially purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the most economic DNA polymerases available.
Special Features
- Robust performance
- Leaves 3'A overhang
- Stable at all storage temperatures
- Best price/quality
- APPLICATIONS
- General PCR
- Genomic analysis
- TA cloning
D116
Ready-to-use, ultra-pure, molecular grade solution made from 100mM each of dATP, dCTP, dGTP, and dTTP stock. The PreMix is designed to reduce "hands-on" time for researchers, thereby lowering the possibility of contamination and error. Furthermore, every lot of dNTP solution is pre-tested for optimal performance. The nucleotides are supplied in sterile, doubly distilled water. Each dNTP has a final concentration of 10mM for a total PreMix dNTP concentration of 40mM.
Special Features
- Ready to use
- Each lot functionally tested
- Purity; at least >99%
- APPLICATIONS
- For RT-PCR, large PCR, and real-time PCR
- Reverse Transcription and mutagenesis