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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Model D3-14 Centipede Wide Gel Electrophoresis System
Gel Size: 23cmW x 14cmL
Footprint: 27cmW x 30cmL x 11cmH
Running Buffer Volume: 800ml
Great for Screening PCR Samples
The Owl Centipede horizontal model D3-14 wide gel system offers a simple, convenient and fast method for screening multiple samples on a single agarose gel. Ideal for screening PCR samples, plasmid preps, restriction mapping and cloning. 25 to 200 samples can be run simultaneously on one gel; producing clear, tight banding patterns with no "smiling".
The Centipede System incorporates Owl's EasyCast casting design with an external caster. The UVT gel tray is fitted with a gasket which allows the tray to fit snugly into the external gel caster to provide a leakproof seal without tape. Sample loading is greatly enhanced using micro well pipette format combs (and a multichannel pipette) engineered specifically for use with this system. Using these combs and a multichannel pipette, samples may be loaded directly from a 96-well plate, 8 or 12 at a time. Sample loading with a multichannel pipette speeds up transfer and reduces the chance of sample order error. The unique electrode placement allows this gel to be run quickly without compromising the resolution.
Comb Options | ||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
D3-MT2C | Micro Well | 50 (2X) | 1.0mm | 3mm | 2µl | 8µl | 14µl | 19µl |
D3-MT2D | Micro Well | 50 (2X) | 1.5mm | 3mm | 3µl | 12µl | 20µl | 29µl |
D3-MTC | Micro Well | 25 (1X) | 1.0mm | 7.5mm | 6µl | 20µl | 34µl | 48µl |
D3-MTD | Micro Well | 25 (1X) | 1.5mm | 7.5mm | 8µl | 30µl | 51µl | 72µl |
D3-WALL | Wall | 1 | 1.5mm | 230 |
- Buffer Chamber
- SuperSafe Lid with Attached Power Supply Leads
- EasyCast Gasketed U.V. Transmissible (UVT) Gel Tray
- External Gel Caster
- 4 Combs: 50 Well, 1.5mm Thick
- Bubble Level
- 3 year warranty
Model D2 Spider Wide Gel Electrophoresis System
Gel Size: 14.4cmW x 10.2cmL
Footprint: 20cmW x 19cmL x 10cmH
Running Buffer Volume: 600ml
Great for Protocols with a Large Number of Samples
This horizontal wide gel system can run from 10 to 80 samples on one gel. The wide gel size allows you to load 15 or 30 samples per row with a multichannel pipette format combs. This economical unit has multi functions: it is great to screen PCR samples or use for routine DNA fragment analysis. The small footprint allows it to fit on the most cluttered lab bench.
This system incorporates Owl's EasyCast casting design with an external caster. The UVT gel tray is fitted with a gasket which allows the tray to fit snugly into the external gel caster to provide a leakproof seal without tape. Sample loading is greatly enhanced using micro well pipette format combs engineered specifically for use with this system. Using these combs and a multichannel pipette, samples may be loaded directly from a 96-well plate, 8 or 12 at a time. This speeds up transfer and reduces the chance of sample order error. The unique electrode placement allows this gel to be run quickly without compromising the resolution.
Comb Options |
||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
D1-10C | Standard | 10 | 1.0mm | 12.2mm | 9µl | 32µl | 55µl | 78µl |
D1-10D | Standard | 10 | 1.5mm | 12.2mm | 14µl | 48µl | 82µl | 117µl |
D1-20C | Standard | 20 | 1.0mm | 5.2mm | 4µl | 14µl | 23µl | 33µl |
D1-20D | Standard | 20 | 1.5mm | 5.2mm | 6µl | 20µl | 35µl | 50µl |
D1-30C | Micro Well | 30 (2X) | 1.0mm | 2.5mm | 2µl | 7µl | 12µl | 17µl |
D1-30D | Micro Well | 30 (2X) | 1.5mm | 2.5mm | 3µl | 10µl | 18µl | 25µl |
D1-40C | Standard | 40 | 1.0mm | 1.7mm | 1µl | 4µl | 8µl | 11µl |
D1-40D | Standard | 40 | 1.5mm | 1.7mm | 2µl | 7µl | 11µl | 16µl |
D1-MTC | Micro Well | 15 (1X) | 1.0mm | 7.2mm | 5µl | 19µl | 32µl | 46µl |
D1-MTD | Micro Well | 15 (1X) | 1.5mm | 7.2mm | 8µl | 28µl | 49µl | 69µl |
D2-RL-25C | Rapid Load | 25(2X) | 1.0mm | 2.5mm | 2µl | 7µl | 12µl | 17µl |
D2-RL-25D | Rapid Load | 25(2X) | 1.5mm | 2.5mm | 3µl | 11µl | 18µl | 26µl |
D2-RL-9C | Rapid Load | 9(1X) | 1.0mm | 7.2mm | 5µl | 19µl | 32µl | 46µl |
D2-RL-9D | Rapid Load | 9(1X) | 1.5mm | 7.2mm | 8µl | 28µl | 48µl | 69µl |
Complete System Includes:
- Buffer Chamber
- SuperSafe Lid with Attached Power Supply Leads
- EasyCast Gasketed U.V. Transmissible (UVT) Gel Tray
- External Multiple Gel Caster
- 4 Combs: (2) 30 Well and (2) 40 Well, 1.5mm Thick
- 3 year warranty
PR1MA™ Mini Gel Electrophoresis System
- Complete system, includes gel box, power supply and casting sets
- Quick and easy gel casting, no tape, clamps or spacers required
- Compact design, footprint: 7 x 5.1 in.
The PR1MA™ Mini Gel electrophoresis system includes all of the equipment that you need to get up and running: gel tank, power supply and two casting sets. Ideal for personal use, small laboratories or the classroom, this gel box/power supply combination is compact and easy to use.
The mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages – 35V, 50V or 100V – and a 99-minute timer. All are set using the membrane keypad.
The safety lid is vented to help dissipate heat generated during electrophoresis and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.
Trays and casting stands for both 10.5 x 6 cm and 5 x 6 cm gels are included with the MyGel Mini system, as are double-sided combs. The smaller tooth combs are compatible with multi-channel pipettes. Casting is quick and easy – just place the tray in the casting stand and pour. The trays feature a dark contrast strip to help visualize wells while loading and lines that fluoresce under UV and blue light to track progress of the run.
Specifications
Output Voltage |
35V, 50V or 100V |
Timer |
0-99 min. or Cont. |
Safety |
Lid interlock |
Max. Buffer |
230 mL |
Gel Capacity |
One: 10.5 x 6 cm |
Weight |
1 lbs | 0.45 kg |
Dimensions |
7.5 x 5.1 x 2.1 in |
Electrical |
115V or 230V, 50-60 Hz |
ENDURO VE20 Vertical Gel Electrophoresis System
Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base
- Large format vertical gel system
- Electroblotting and gel electrophoresis in same universal gel tank
- Quick assembly system for ease of use
- Complete system allows casting of gels and running of gels at same time increasing efficiency
The ENDURO VE20 is Labnet's new large format vertical gel electrophoresis system. It distinguishes itself in its flexibility of applications and ease of gel casting and set-up requiring only 4 screws to secure 4 gels. The same gel tank can house a large format electroblotting assembly sold separately. This system is ideal for first- and second dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis.
Innovative screw-clamp technology consists of only four screws to secure up to four 20 x 20cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the center to eliminate plate bowing and gel compression, while maintaining a leak-proof seal during casting.
NO TANK TOP ASSEMBLY
A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber.
CASTING ADVANTAGES
- Dual purpose PAGE insert eliminates time consuming transfer of glass plates between separate casting and gel running components.
- Glass plates with bonded spacers assure clean well formation, correct alignment for leak-free casting and eliminates manually aligning spacers.
- Casting stand design ensures leak-free casting even if glass plates are misaligned.
Specifications:
Plate Dimensions (w x h x t) |
20 x 20 x 0.4 cm |
Standard Spacer (w x h) |
2 x 20 cm |
IPG Spacer (w x h) |
0.6 x 20 cm |
Unit (w x d x h) |
30 x 18 x 27 cm |
Unit weight |
5.5 lbs |
Number of gels |
4 |
Total volume inner buffer chamber |
640 mL |
Total buffer volume for 2 gels |
5.3 L |
Total buffer volume for 4 gels |
4.8 L |
Standard run time for SDS-PAGE without coolling |
4-5 hr |
Standard run time for SDS-PAGE with coolling |
3-4 hr |
Order# |
Description |
E2020 |
Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base |
E2020-CU |
Enduro VE20 Dual Casting System, includes 20 x 20 cm Dual, 2 sets of glass plates, 1 mm think bonded spacers, 2 x 24 sample, 1 mm thick combs, cooling coil, dummy plate; also includes caster and external casting |
E2020-IEF |
Enduro VE20 IEF Conversion Kit for 18 cm IPG strips and Tube gels |
E2020-TB |
Enduro VE20 Blotting System, 20 x 20 cm System Including tank and lid, 4 cassettes, 18 fibre pads, cooling coil |
Screen up to 7 Samples on 1 Gel in Less than 5 Minutes
The Owl Pollywog horizontal micro gel electrophoresis system is designed for quick restriction fragment analysis. Glass slides, 2" x 3", are used as the gel tray. Thin gels can be poured and formed by surface tension directly on the glass slide. Because the gel is so thin, a complete gel run will last 5 to 10 minutes or gels can be cast and solidified in less than 10 minutes using the available caster (see below). This system is designed for quick rapid screening and can be used to check a few PCR samples before you load a large number of samples on a larger gel, saving time.
Comb Options | ||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
C2-7C | Standard | 7 | 1.0mm | 4.8mm | 4µl | 13µl | 22µl | 31µl |
C2-7D | Standard | 7 | 1.5mm | 4.8mm | 5µl | 19µl | 32µl | 46µl |
Complete System Includes:
- Buffer chamber
- Lid
- Power supply leads
- Gel casting tray
- Casting slides (10)
- 1 comb, 7 well, 1.5 mm
- 3 year warranty
Gel Size: 12cmW x 14cmL
Footprint: 18cmW x 24.5cmL x 9.5cmH
Running Buffer Volume: 800ml
The Owl EasyCast horizontal B2 mini gel system provides the most flexibility in a very small footprint. The longer gel length and the two comb slots give you the flexibility of running 8 to 48 samples on one gel. The optional multi load tray can increase the maximum number of samples to 108 in a multichannel pipette format. The simple to use casting method, and multiple comb and tray options, makes this Owl.s most popular system.
Owl offers a wide range of combs. The two comb slots on the U.V. Transmissible (UVT) gel tray, double your sample capacity by doubling the number of sample wells. Heavy duty, one piece combs eliminate comb assembly. UVT gel trays are silk screened with a florescent ruler for easy measurement of bands. This system is available with optional buffer exchange ports, catalog number B2-BP, for use with an external recirculation pump.
Comb Options |
||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
B2-12 | Double Sided | 12 | 1.0 1.5mm |
7.2 7.2mm |
5 8µl |
18 28µl |
32 49µl |
46 69µl |
B2-16 | Double Sided | 16 | 1.0 1.5mm |
5.4 5.4mm |
4 6µl |
14 21µl |
24 36µl |
34 52µl |
B2-20 | Double Sided | 20 | 1.0 1.5mm |
3.9 3.9mm |
3 4µl |
10 15µl |
18 26µl |
25 37µl |
B2-24 | Double Sided | 24 | 1.0 1.5mm |
3 3.0mm |
4 5µl |
13 19µl |
21 32µl |
30 45µl |
B2-8C | Standard | 8 | 1.0mm | 12.5mm | 9µl | 33µl | 56µl | 80µl |
B2-8D | Standard | 8 | 1.5mm | 12.5mm | 14µl | 49µl | 84µl | 120µl |
B2-PREP | Prep | 2 | 1.5mm | 106/5mm | 120/5.5µl | 415/20µl | 710/34µl | 1000/48µl |
B2-RL-25C | Rapid Load | 25(2X) | 1.0mm | 2.5mm | 2 µl | 7µl | 12µl | 17µl |
B2-RL-25D | Rapid Load | 25(2X) | 1.5mm | 2.5mm | 3µl | 11µl | 18µl | 26µl |
B2-RL-9C | Rapid Load | 9(1X) | 1.0mm | 7.2mm | 5µl | 19µl | 32µl | 46µl |
B2-RL-9D | Rapid Load | 9(1X) | 1.5mm | 7.2mm | 8µl | 28µl | 49µl | 69µl |
B2-WALL | Wall | 1 | 1.5mm | 120mm |
Complete System Includes:
Model B1 EasyCast Mini Gel Electrophoresis System
Gel Size: 9cmW x 11cmL
Footprint: 15cmW x 22cmL x 9.5cmH
Running Buffer Volume: 600ml
Fits Most Teaching Applications
The Owl EasyCast model B1 horizontal mini gel system is the perfect small device. Run from 5 to 34 samples in this functional system. The unit is economical enough for every researcher to have one at their workstation. The durability and ease of use makes this the perfect choice for students and high volume researchers.
Owl offers a wide range of combs. The two comb slots on the U.V. transmissible (UVT) gel tray, double your sample capacity by doubling the number of sample wells. Heavy duty, one piece combs eliminate comb assembly. UVT gel trays are silk screened with a florescent ruler for easy measurement of bands. This system is available with optional buffer exchange ports, catalog number B1-BP, for use with an external recirculation pump.
Comb Options |
||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
B1-10 | Double Sided | 10 | 1.0 1.5mm |
6.8 6.8mm |
5 8µ1 |
18 27µ1 |
31 46µ1 |
43 65µ1 |
B1-12C | Standard | 12 | 1.0mm | 5.4mm | 4µ1 | 14µ1 | 24µ1 | 34µ1 |
B1-12D | Standard | 12 | 1.5mm | 5.4mm | 6µ1 | 21µ1 | 36µ1 | 52µ1 |
B1-14 | Double Sided | 14 | 1.0 1.5mm |
4.4 4.4mm |
3 5µ1 |
12 17µ1 |
20 30µ1 |
28 42µ1 |
B1-5C | Standard | 5 | 1.0mm | 15.4mm | 12µ1 | 40µ1 | 69µ1 | 99µ1 |
B1-5D | Standard | 5 | 1.5mm | 15.4mm | 17µ1 | 61µ1 | 104µ1 | 147µ1 |
B1-8C | Standard | 8 | 1.0mm | 9mm | 7µ1 | 24µ1 | 41µ1 | 57µ1 |
B1-8D | Standard | 8 | 1.5mm | 9m | 10µ1 | 35µ1 | 61µ1 | 86µ1 |
B1-PREP | Prep | 2 | 1.5mm | 78/5mm | 90/6µ1 | 310/19µ1 | 525/32µ1 | 750/46µ1 |
B1-RL-17C | Rapid Load | 17(2X) | 1.0mm | 2.5mm | 2µ1 | 7µ1 | 12µ1 | 17µ1 |
B1-RL-17D | Rapid Load | 17(2X) | 1.5mm | 2.5mm | 3µ1 | 11µ1 | 18µ1 | 26µ1 |
B1-RL-9C | Rapid Load | 9(1X) | 1.0mm | 7.2mm | 5µ1 | 19µ1 | 32µ1 | 46µ1 |
B1-RL-9D | Rapid Load | 9(1X) | 1.5mm | 7.2mm | 8µ1 | 28µ1 | 49µ1 | 69µ1 |
Complete System Includes:
- Buffer Chamber
- SuperSafe Lid with Attached Power Supply Leads
- EasyCast Gasketed U.V. Transmissible (UVT) Gel Tray
- 2 Combs: 10 & 14 Well, Double Sided, 1.0/1.5mm Thick
- 3 year warranty
Model B1A EasyCast Mini Gel Electrophoresis System
Gel Size: 7cmW x 8 cmL
Footprint: 10.5cmW x 16 cmL x 9.5 cmH
Running Buffer Volume: 400ml
Ideal Size for Every Lab
The EasyCast horizontal mini gel system is designed to provide flat, even banding patterns and consistent results with less hassle. This is the perfect size to determine molecular weight ranges of PCR fragments or larger sized DNA molecules. The all in one design allows you the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment. Run from 5 to 24 samples in 15 minutes.
The two comb slots on the U.V. Transmissible (UVT) gel tray, double your sample capacity by doubling the number of sample wells. Heavy duty, one piece combs eliminate comb assembly. UVT gel trays are silk screened with a florescent ruler for easy measurement of bands. This system is available with optional buffer exchange ports, catalog number B1A-BP, for use with an external recirculation pump.
Comb Options | ||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
B1A-10 | Double Sided | 10 | 1.0 1.5mm |
4.8 4.8mm |
4 5µl |
13 19µl |
21 32µl |
30 46µl |
B1A-12 | Double Sided | 12 | 1.0 1.5mm |
3.7 3.7mm |
3 4 |
10 14 |
16 25 |
23 35 |
B1A-5C | Standard | 5 | 1.0mm | 11.3mm | 8µl | 30µl | 51µl | 72µl |
B1A-5D | Standard | 5 | 1.5mm | 11.3mm | 32µl | 64µl | 95µl | 127µl |
B1A-6 | Double Sided | 6 | 1.0 1.5mm |
9.1 9.1mm |
17 26µl |
34 51µl |
51 77µl |
68 102µl |
B1A-8 | Double Sided | 8 | 1.0 1.5mm |
6.4 6.4mm |
5 7µl |
17 25µl |
29 43µl |
41 61µl |
B1A-PREP | Prep | 2 | 1.5mm | 57/5mm | 64/6µl | 225/19µl | 385/32µl | 545/46µl |
Complete System Includes:
- Buffer Chamber
- SuperSafe Lid with Attached Power Supply Leads
- EasyCast Gasketed U.V. Transmissible (UVT) Gel Tray
- 2 Combs: 6 & 10 Well, Double Sided, 1.0/1.5mm Thick
- 3 year warranty
Model B1 EasyCast Mini Gel Electrophoresis System
Gel Size: 9cmW x 11cmL
Footprint: 15cmW x 22cmL x 9.5cmH
Running Buffer Volume: 600ml
Fits Most Teaching Applications
The Owl EasyCast model B1 horizontal mini gel system is the perfect small device. Run from 5 to 34 samples in this functional system. The unit is economical enough for every researcher to have one at their workstation. The durability and ease of use makes this the perfect choice for students and high volume researchers.
Owl offers a wide range of combs. The two comb slots on the U.V. transmissible (UVT) gel tray, double your sample capacity by doubling the number of sample wells. Heavy duty, one piece combs eliminate comb assembly. UVT gel trays are silk screened with a florescent ruler for easy measurement of bands. This system is available with optional buffer exchange ports, catalog number B1-BP, for use with an external recirculation pump.
Comb Options |
||||||||
Catalog Number |
Comb Type |
Number of Teeth |
Thickness of Teeth |
Width of Teeth |
Recommended Loading Volumes | |||
0.25cm | 0.5cm | 0.75cm | 1.0cm | |||||
B1-10 | Double Sided | 10 | 1.0 1.5mm |
6.8 6.8mm |
5 8µ1 |
18 27µ1 |
31 46µ1 |
43 65µ1 |
B1-12C | Standard | 12 | 1.0mm | 5.4mm | 4µ1 | 14µ1 | 24µ1 | 34µ1 |
B1-12D | Standard | 12 | 1.5mm | 5.4mm | 6µ1 | 21µ1 | 36µ1 | 52µ1 |
B1-14 | Double Sided | 14 | 1.0 1.5mm |
4.4 4.4mm |
3 5µ1 |
12 17µ1 |
20 30µ1 |
28 42µ1 |
B1-5C | Standard | 5 | 1.0mm | 15.4mm | 12µ1 | 40µ1 | 69µ1 | 99µ1 |
B1-5D | Standard | 5 | 1.5mm | 15.4mm | 17µ1 | 61µ1 | 104µ1 | 147µ1 |
B1-8C | Standard | 8 | 1.0mm | 9mm | 7µ1 | 24µ1 | 41µ1 | 57µ1 |
B1-8D | Standard | 8 | 1.5mm | 9m | 10µ1 | 35µ1 | 61µ1 | 86µ1 |
B1-PREP | Prep | 2 | 1.5mm | 78/5mm | 90/6µ1 | 310/19µ1 | 525/32µ1 | 750/46µ1 |
B1-RL-17C | Rapid Load | 17(2X) | 1.0mm | 2.5mm | 2µ1 | 7µ1 | 12µ1 | 17µ1 |
B1-RL-17D | Rapid Load | 17(2X) | 1.5mm | 2.5mm | 3µ1 | 11µ1 | 18µ1 | 26µ1 |
B1-RL-9C | Rapid Load | 9(1X) | 1.0mm | 7.2mm | 5µ1 | 19µ1 | 32µ1 | 46µ1 |
B1-RL-9D | Rapid Load | 9(1X) | 1.5mm | 7.2mm | 8µ1 | 28µ1 | 49µ1 | 69µ1 |
Complete System Includes:
- Buffer Chamber
- SuperSafe Lid with Attached Power Supply Leads
- EasyCast Gasketed U.V. Transmissible (UVT) Gel Tray
- 2 Combs: 10 & 14 Well, Double Sided, 1.0/1.5mm Thick
- 3 year warranty
Bullseye 1Kb DNA Ladder, Logic
- Ready to use
- Contains 16 DNA bands: 100bp-10Kb
- 920ng DNA/6µl/loading
- Easy quantification of DNA fragments
- Stable at room temperature
- Supplied with 6x sample loading buffer
Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.
Size: 1200µl
Storage: Store at -20°C.
Concentration: 920ng/6µl
Loading Buffer Composition:
10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol
Usage: Add 6µl of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6µl of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.
Need a great DNA Ladder at a great price? Try PR1MA! Click here to order.
- A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
- The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
- The approximate mass of DNA in each band is provided (0.5µg a load) for approximating the mass of DNA in comparably intense samples of similar size.
Source:
- PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10mM EDTA.
Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 µg/mL
Package: 50 µg/500 µL
Recommended Load: 5 µL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)
Storage:
- Store at 25°C for 6 months
- Store at 4°C for 12 months
Store at -20°C for 24 months
Bullseye 100bp DNA Ladder
- Ready to use
- Contains 11 DNA bands: 100-1500bp.
- Clearly identifiable 500bp band as reference
- 500ng DNA/6µl/loading
- Easy to load
- Stable at room temperature
- Supplied with 6x sample loading buffer
Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6µl will yield at least 30ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.
Size: 1200µl
Storage: Store at -20°C.
Concentration: 500ng/6µl
Loading Buffer Composition:
10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol
Usage: Add at least 6µl Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6µl of ladder, depending on well size and level of intensity needed to visualize the bands.
Please give us a call for a sample.