• Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 µL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 µL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator   

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2X Master Mix
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy

Supplied as a ready-to-use 2X master mix, PR1MA qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. PR1MA Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.


*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

PR1MA Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low copy and long PCR
• Enzyme of choice for TA cloning

PR1MA Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number and long PCR.

The 3' -5' exonuclease activity of the proofreading enzyme produces fragments suitable for TA cloning. PR1MA Taq Plus is provided with a 5X buffer with dNTPs or in a convenient one tube 2X Master Mix.

PR1MA Taq Start Taq DNA Polymerase

PR1MA Taq Plus Master Mix, 2X Concentration

• Same high efficiency for multiplex and singleplex reactions
• Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy
• Compatible with popular hydrolysis and beacon probes
• Ready to use 2X mastermix
• Early Ct values and detection across a broad dynamic range

Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes PR1MA Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.

PR1MA qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.
*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

PR1MA One-Step RT-PCR Kit

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

PR1MA qMAX™ cDNA Synthesis Kits

PR1MA now offers two cDNA Synthesis Kits, to meet a range of requirements.

The original cDNA Synthesis Kit is a 2-tube format for easy reaction set up and is ideal for 4 pg to 0.5 µg of input RNA.  One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 

The 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers.  This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
 
 
 
PR1MA qMax cDNA Synthesis Kits

PR1MA qMax First Strand cDNA Synthesis Flex Kits 

PR1MA dNTPs

• Supplied as a ready-to-use 40mM mix or a set of 4 separate 100mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
 
 
 
PR1MA dNTP Mix

PR1MA dNTP Set 

PR1MA Hot Start Taq

• Exceptional sensitivity for low copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility

Engineered for controlled polymerase activity, PR1MA Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye.  The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

PR1MA High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers

For applications requiring highly accurate amplification, choose PR1MA High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets

PR1MA High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, an antibody mediated hot start mechanism, and a proof-reading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.