PCR/qPCR

StripSpin 12 Mini Centrifuge

• Unique rotor for 12-strip PCR tubes
• Also can be used with 8-strip and individual PCR tubes
• Starts and Stops with opening/closing the lid
• Tiny benchtop footprint and affordability
• Place a StripSpin at the bench, thermal cycler & water bath for convenience.

Specifications:

Speed:	5,500 rpm / 2,000 xg
	4 x 12 position PCR strips
Capacity:	4 x 8 position PCR strips
	48 x 0.2ml PCR tubes
Dimensions:	5.5 x 7.9 x 4.4 in.
(w x d x h)	14 x 20 x 11.2 cm
Weight:	2.2 lbs. / 1 kg
Electrical:	Universal Voltage
	100-240V, 50-60 Hz, 300W
Warranty:	1 Year

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets

PR1MA High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, an antibody mediated hot start mechanism, and a proof-reading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

• Dimensional uniformity for superior thermal transfer
• AVST-01 flat caps are optically clear and ideal for qPCR
• Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

Please give us a call for a sample!

PR1MA High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

Pryme PCR Standard Individual Tubes 0.2 & 0.5 mL

Thinwall flattop or domed capped with attached tubes are available in 0.2 mL and 0.5 mL volumes.   0.2 mL tubes are suitable for use in all thermal cyclers with standard 0.2 mL block formats.  0.5 mL tubes are suitable for use in all thermal cyclers with standard 0.5 mL block formats.  Available in clear tubes, individual colors, and assorted colors.  The thin wall feature accelerates heat transfer, providing shorter cycle times.

PR1MA qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

PR1MA qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and reverse transcriptase. Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  PR1MA Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95°C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

qMAX™ Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

**New Name, Same Great Product!**

PR1MA SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details

Protocol:  Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration:  0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage:  SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production:  SmartCheck™ ladders are produced from proprietary plasmids, digested to completion.  A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.
 
 

Item	Description	Volume
PR4005-100	PR1MA SmartCheck™ 50bp DNA Ladder	500 µL / 100 Lanes
PR4005-500	PR1MA SmartCheck™ 50bp DNA Ladder	5 x 500 µL / 500 Lanes
PR4010-100	PR1MA SmartCheck™ 100bp DNA Ladder	500 µL / 100 Lanes
PR4010-500	PR1MA SmartCheck™ 100bp DNA Ladder	5 x 500µL / 500 Lanes
PR4100-100	PR1MA SmartCheck™ 1kb DNA Ladder	500 µL / 100 Lanes
PR4100-500	PR1MA SmartCheck™ 1kb DNA Ladder	5 x 500 µL / 500 Lanes

PR1MA Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

Item	Description	Volume
PR1300-MG-S	PR1MA 1 Hour Mammalian Genotyping Kit	8 Reactions (Sample)
PR1300-MG-80	PR1MA 1 Hour Mammalian Genotyping Kit	80 Reactions
PR1300-MG-400	PR1MA 1 Hour Mammalian Genotyping Kit	400 Reactions
PR1300-MG-800	PR1MA 1 Hour Mammalian Genotyping Kit	800 Reactions

 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

Item	Description	Volume
PR1301-HSR-400	PR1MA Genotyping Hot Start Master Mix, 2X Conc., Red Dye	400 Reactions

 
 

IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

IB42300	PCR Grade Water,nuclease free,1.8 mL vial
IB42301	PCR Grade Water,nuclease free,(20)x 1.8 mL vial
IB42302	PCR Grade Water,nuclease free,(50) x 1.8 mL vial
IB42303	PCR Grade Water,nuclease free,(100) x 1.8 mL vial

PurePlus® PCR Plates

• PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
• Our plates are lot certified free of Human DNA contamination
• Our plates are tested for ATP lot by lot to ensure pure consistent product
• Broad product range. Over 18 styles of plates fit most major thermocyclers
• These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.

Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
 

MIDSCI #	VWR #
3983-520-000-9	82006-678
3983-520-010-9	89218-288
3984-520-000-9	89218-294
3961-520-000-9	89218-296
3962-520-000-9	89218-298
3962-521-000-9	10755-256
3971-520-000-9	82006-650
3976-520-000-9	82006-664
3978-520-000-9	82006-704
3978-520-010-9	89218-292
3978-522-000-9	82006-714
3978-524-000-9	82006-710
3978-526-000-9	82006-712
3978-527-000-9	82006-706
3978-528-000-9	82006-708
3978-529-000-9	82006-718
3963-520-000-9	83009-676
3967-520-000-9	83009-668
3968-520-000-9	83009-672
3972-520-000-9	89049-178
3972-520-010-9	89218-290
3973-520-000-9	83007-374
3973-529-000-9	83007-372
3974-520-000-9	37001-272
3977-520-000-9	82006-636
3977-522-000-9	82006-646
3977-526-000-9	82006-644
3977-527-000-9	82006-638
3977-528-000-9	82006-640
3977-529-000-9	82006-648
3979-520-000-9	93001-100