PCR/qPCR

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

Our rack is perfect for pre- or post-PCR® sampling applications. Sturdy polypropylene rack accommodates individual 0.2mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with imprinted numbers and letters. Features a removable hinged lid. Available in natural, blue, green, pink, yellow, or assorted colors (one of each). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.

PR1MA One-Step RT-PCR Kit

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

PR1MA qMAX™ cDNA Synthesis Kits

PR1MA now offers two cDNA Synthesis Kits, to meet a range of requirements.

The original cDNA Synthesis Kit is a 2-tube format for easy reaction set up and is ideal for 4 pg to 0.5 µg of input RNA.  One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 

The 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers.  This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
 
 
 
PR1MA qMax cDNA Synthesis Kits

PR1MA qMax First Strand cDNA Synthesis Flex Kits 

Designed for laboratories with medium to high throughput needs, the TC9639 Thermal Cycler provides fast, reliable results. Featuring a large, touch screen interface, a next-generation program wizard and a unique multi-format block, this unit makes it easier than ever to get up and running.

A large, full color touch screen serves as the operations center where all functions are accessed. Starting at the "Home" screen, simply touch an icon to view the last run, enter the program library or use the program wizard to quickly enter a new protocol.

Designed with extensive user feedback, the program wizard quickly and easily guides users through protocol set-up, all in a single screen. Time and temperature increments can be built into programs as well as slower ramp rates. Storage capabilities are unlimited; programs can be stored in public folders, password protected folders or on a flash drive.

The fully adjustable heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Heating and cooling of the block are precisely and accurately controlled within exceptionally tight tolerances by a proprietary algorithm that simulates sample temperature.

With the multi-format block, there is no need for the expense of multiple interchangeable blocks. The TC 9639 has the flexibility to accept 96 x 0.2ml tubes, a 96 well plate or 39 x 0.5ml tubes, all in the same sample block. An in-situ adapter is available for use with slides. A 384 well version is available.

• Gradient capabilities- ideal for protocol optimization
• Intuitive touch screen control- Works even when wearing gloves!
• Upgraded software- Even easier to use than before!
• No need for interchangeable blocks- multi-format accepts:
• 0.2ml tubes or strips (96)
• 0.5ml tubes (39)
• 96 well PCR plate

PR1MA dNTPs

• Supplied as a ready-to-use 40mM mix or a set of 4 separate 100mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
 
 
 
PR1MA dNTP Mix

PR1MA dNTP Set 

Mystaire® CleanPrep™ dead air boxes are circulation-free enclosures that establish an ideal work area to protect against contamination in sensitive PCR amplification reactions. CleanPrep™ workstations are constructed from thermally-fused polypropylene that offers exceptional chemical resistance, does not adsorb moisture and can be easily cleaned and decontaminated between amplifications using a variety of disinfectant solutions.Seamless polypropylene design for easy decontamination between amplifications.

FEATURES:

1. Clear polycarbonate access with overlap and safety position switch
2. UV shelf with integrated pipette holder - accommodates four pipettors
3. High intensity fluorescent lamps and shortwave UV light
4. Available in 36 and 48 inch widths with nominal 24 inch depth
5. Circulation-free enclosure for PCR applications

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

PR1MA Hot Start Taq

• Exceptional sensitivity for low copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility

Engineered for controlled polymerase activity, PR1MA Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye.  The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

• View DNA migration and separation as it happens
• Blue light illumination - won't damage DNA
• Capture high resolution images without removing the gel from the tank

The myGel InstaView comes complete with everything needed to cast, run, and view mini electrophoresis gels, including an integrated power supply.

Gels are easily poured using the supplied trays, combs and leak-proof casting stand.  One large (10.5x10cm) or one small (10.5x6cm) gel is accommodated by the gel tank.

The power supply connects directly into the gel tank.  With no more leads to contend with the connection is guaranteed to be right, every time.  Output voltage can be adjusted to 35V, 50V, or 100V and run time can be set for up to 99 minutes.  If the lid is not properly in place a magnetic interlock between the gel tank and the lid prevents current from running through the system.

Once the system is set up, slide the mini LED transilluminator into place under the tank and at any time turn it on to view the run in real time.  An array of blue LEDs emits bright blue light at a wavelength of 465nm for optimal excitation of common green fluorescent stains such as SmartGlow, SYBYR Green and Gel Green, without damaging DNA.  An orange filter is incorporated into the lid for visualizing DNA bands eliminating the need for additional equipment or special glasses.

To document results, place the included gel imaging enclosure over top of the myGel InstaView and use the camera ap on any smart phone to capture high resolution images without removing the gel from the tank.  Images can easily be shared via email, text, or printed.

Technical Data

InstaView Gel Tank

The InstaView Gel Tank is the perfect choice for labs that prefer to use a separate power supply.  The InstaView Gel Tank accommodates one large (10.5x10cm) or one small (10.5x6cm) gel tray and is supplied with trays, combs and a leak-proof casting stand. The gel tank lid includes attached power leads which prevent the unit from operating without the lid properly in place. Any standard power supply can be connected, including the Accuris myVolt Mini and myVolt Touch.

Technical Data

System & Accessories:

E1201  myGel InstaView Electrophoresis System, includes gel tank with orange filter, direct connect power supply, E1200-CS1 casting set (see below), Mini SmartBlue Transilluminator and imaging enclosure

E1200 - InstaView Gel Tank, includes tank with orange filter, C1200-CS1 casting set (see below), mini UV
transilluminator and imaging enclosure (power supply not included)

E1200-CS1 - Additional casting set, includes casting stand, divider, 2 trays for 10.5x6cm gels, one tray for 10.5x10cm gels, 2 double-sided combs -12/22 teeth

E1101-COMB1 - Additional double-sided combs, 12/22 teeth, pack of 2

PR1MA High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers

For applications requiring highly accurate amplification, choose PR1MA High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.