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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Zone-Free™
Zone-Free is a multilayer film consisting of a top pierceable clear polyethylene film and a white polypropylene sublayer with 96 circular areas void of adhesive.
- Pierceable
- Offers excellent chemical resistance
- Samples have minimal contact with adhesive due to the adhesive-free zones
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
Affordable price & shipped at room temperature.
A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.
- 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
- This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
- The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
- The elongation velocity is 0.9~1.2kb/min (70~75°C).
- It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.
Features:
- Convenient: Just add primers and template DNA
- High yields of PCR products with minimal optimization.
- High efficiency: saves your time by simplifying the process
- Reproducible: lower contamination risk and pipetting error.
Applications:
- High-throughput PCR.
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
Contents:
2X Taq Mix 1ml
Store at -20°C
For research use only
ThermalSeal® An Easier to Remove Sealing Film
ThermalSeal® features a moderate strength adhesive for ease of removal.
- Certified DNase- & RNase- free
- Only recommended for structurally rigid polypropylene PCR plates that don’t warp
- A less rigid polypropylene film that minimizes aerosols upon removal
- Features good chemical resistance
- PCR
- General storage
- -40°C to +120°C
Due to supply constraints 100-SEAL-PLT, STR-SEAL-PLT, XTR-100 and XTR-LG-100 are out of stock.
STR-SEAL-PLT alternative is GPS-PP-100.
PP-100 substrate is polypropylene instead of polyester for the STR-SEAL-PLT. GPS-PP-100 is designed for the same application – ELISA/Incubation/General Storage.
100-SEAL-PLT alternative is TTS-100.
XTR-100 and XTR-LG-100 alternatives are XTR2-100 & XTR2-LG-100.
ELISA, Incubation, and Short Term Incubation Sealing Films
All films on this page are designed for ELISA, Incubation, and Short Term Incubation Sealing Films. If you are looking for something specific please consult our selection guides by attribute or application on this page. All films are certified DNase- & RNase-Free.
Specific film descriptions:
1x8 MiniStrips (GP-CS1X8-400 and GPS-CS1X8-400) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.
General Purpose (GP-PP-100 and GPS-PP-100) polypropylene lms oer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.
Pre-scored lm (SP2-RL-500) on a roll is a more economical and ecient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted lm. Self-standing, non-slip supports hold the pre-scored lm roll securely in place for quick and easy bench- top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn o or fed directly into a waste basket for disposal.
The SealMate SealPlate Starter Kit (SM-KIT-SP and replacement rolls) features a re-usable dispenser and two rolls of perforated lm. The dispenser facilitates the quick and easy dispensing of lm from a roll allowing microplates to be sealed three times faster than using sheeted lms. Cost-eective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes lm contamination.
SealPla te (100-SEAL-PLT and STR-SEAL-PLT) minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the edge eect which can occur with plate lids (e.g. greater evaporation in the outside plate wells).
SealPlate Color Tabs (SP-IDx-100) Each end-tab features a colored dot and white writing area. An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identication. The lm sheet can be removed from the plate without disturbing the identication label due to the nely perforated end-tabs. Choose from 5 dierent individual colors or the assortment pack.
SealPlate 2x8 MiniStrips (SP-2X8-50 and SPS-2X8-50) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip- well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed.
ValueSeal (SP-VS-100) features smaller dimensions and a single non-perforated end-tab to provide a more cost-eective alternative to protect samples from evaporation, external contamination, dust and particulates.
X-Pierce™ Sealing Films for Re-sealability and Easy Access Without Pipet Tip Coring or Adhesive Fouling
X-Pierce™ film is designed for short-term processing and storage of samples that require multiple access to wells by pipet tips or robotic probes.A pre-cut “X” over each well creates bendable flaps that provide sample access without coring or adhesive fouling of tips or probes. Ideal for COVID-19 sample collection in deep-well plates using swabs.
- Pierceable
- Printed circles aid in the alignment of the film to the plate
- Printed alphanumerics identify each well of a 96-well plate
- Reseals after piercing for continued sample protection
Helpful Tips
- To help position the film to the plate, align wells A-1 and H-12
Applications
- Short-term processing and storage at ambient temperatures
- Robotics
Temperature Range
- -40°C to +90°C
AeraSeal™ SealMate™ Rolls for Fast and Efficient Microplate Sealing
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate™ dispenser minimizes film contamination.
- Environmentally friendly; no paper liner to remove and discard; utilizes approximately 59% less material than AeraSeal™ sheeted film
- Each sheet is separated by an adhesive-free, perforated, color-coded red band
- The smooth lower surface of the dispenser can be used to seal the film to the plate
- Dispenser protects unused seals from contamination
- Lower support prop prevents dispensing bill from contacting surfaces to prevent contamination
- Replacement rolls can be easily installed by releasing the locking clasps on the dispenser
- Designed to facilitate air and CO2 exchange
- Pore size from 10 µm to 50 µm
- Moisture vapor transmission rate = 4,200g/m²/24hr
- Easy-to-pierce with serological pipets and pipet tips for direct sample recovery
- Cell culture
- Tissue culture
- Bacterial cultures
- -20°C to +80°C
ValueSeal™ Sealing Film
The most economical film for general laboratory use.- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
BrightMax™ White, Light-reflecting, Vinyl Sealing Film
Designed for use with white microplates.
- The light reflective properties on clear-bottom plates maximize the sensitivity of bioluminescence and chemiluminescence assays in bottom-reading luminometers
- Maximizes intensity and signal-to-noise ratios in luminescence assays
- May be used as a backing matrix for microscopy of punch-out samples for filter plates (e.g. ELISPOT assays)
- Features a single split liner for easy application
Applications
- Luminescence
- Fluorescence
- Microscopy
- -40°C to +80°C
TiterSeal™ Sealing Film
- Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells)
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
SealPlate® ColorTab™ Sealing Film for Sample Identification and Plate Labeling
An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identification. The film sheet can be removed from the plate without disturbing the identification label due to the finely perforated end-tabs. Choose from 5 different individual colors or the assortment pack.
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
Temperature Range
- -40°C to +120°C
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